FastQCFastQC Report
Thu 2 Feb 2017
SRR4062122_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062122_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703485
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28860.1694174002119185No Hit
TATCAACGCAGAGTACTTTTTTTTT19410.11394288766851483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1600.014.2509716
GTATACG400.00529038514.2455261
GGTATCA6900.014.1767071
CGAGCCG2850.013.66719515
GTAATAC852.7030728E-713.4103133
ACGAACG1750.013.02907515
GTATCAA18700.012.391831
CCGATAA1950.012.1803169
TTAGGTT550.003069788712.090314
AGGCCCG3400.012.01623110
GTTCTAA951.0439053E-611.9962321
AGTCCGA802.8630133E-511.87650710
TAATTCC2800.011.8744134
GATAACG1950.011.6937911
TAACGAA1950.011.692759513
AATTCCG1950.011.6917295
ACCGTCG2850.011.667468
CGATAAC2050.011.58683610
ATTCCGA2050.011.5861546
CGTTATT1657.2759576E-1211.5128892
Produced by FastQC (version 0.11.5)