##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062122_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1703485
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178943166508656	32.0	32.0	32.0	32.0	32.0
2	30.79441028245039	32.0	32.0	32.0	32.0	32.0
3	30.827909843644058	32.0	32.0	32.0	32.0	32.0
4	30.839279477072	32.0	32.0	32.0	32.0	32.0
5	30.743370795751062	32.0	32.0	32.0	32.0	32.0
6	34.35170899655706	36.0	36.0	36.0	32.0	36.0
7	34.303510157119085	36.0	36.0	36.0	32.0	36.0
8	34.27042797559121	36.0	36.0	36.0	32.0	36.0
9	34.38770344323549	36.0	36.0	36.0	32.0	36.0
10	34.108967792495974	36.0	36.0	36.0	32.0	36.0
11	34.36680217319201	36.0	36.0	36.0	32.0	36.0
12	34.21132443197328	36.0	36.0	36.0	32.0	36.0
13	34.286433986797654	36.0	36.0	36.0	32.0	36.0
14	34.19744288913609	36.0	36.0	36.0	32.0	36.0
15	34.13997070710925	36.0	36.0	36.0	32.0	36.0
16	34.14000651605385	36.0	36.0	36.0	32.0	36.0
17	34.07496397091844	36.0	36.0	36.0	32.0	36.0
18	34.07467515123409	36.0	36.0	36.0	32.0	36.0
19	34.08609644346736	36.0	36.0	36.0	32.0	36.0
20	34.07282130456095	36.0	36.0	36.0	32.0	36.0
21	34.04849470350487	36.0	36.0	36.0	32.0	36.0
22	34.029190160171645	36.0	36.0	36.0	32.0	36.0
23	33.99017836963636	36.0	36.0	36.0	32.0	36.0
24	33.96197677114856	36.0	36.0	36.0	32.0	36.0
25	33.62250915035941	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	28.0
5	140.0
6	358.0
7	80.0
8	280.0
9	208.0
10	121.0
11	52.0
12	54.0
13	79.0
14	203.0
15	300.0
16	525.0
17	681.0
18	1022.0
19	1262.0
20	1881.0
21	2713.0
22	3986.0
23	5840.0
24	8516.0
25	12623.0
26	17824.0
27	24165.0
28	33739.0
29	46112.0
30	63519.0
31	88600.0
32	127935.0
33	181148.0
34	379379.0
35	700112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42405478673631	17.504364599953742	12.228159095685415	25.84342151762453
2	16.832474210161426	19.162937537900333	37.012799086106625	26.991789165831616
3	18.79087231049924	22.676632955204536	28.414078038082135	30.118416696214094
4	13.142381425135225	15.37593987169544	34.658434759533165	36.82324394363617
5	15.42094244834562	35.77381004812365	33.013879224031875	15.791368279498858
6	35.283523700155364	34.46774141440248	16.219546937732574	14.029187947709577
7	30.954657402145752	30.043386306193437	20.02445805314602	18.97749823851479
8	28.61281629074361	32.481409526361624	18.81766115563579	20.088113027258977
9	27.14654627263559	14.398151304360091	18.526391634793278	39.92891078821104
10	16.751770306578834	26.261257104297464	30.581939610606028	26.405032978517674
11	37.09025777101638	21.20404829407681	21.77377685004535	19.931917084861457
12	24.460233751760427	23.47592300439901	28.233072276834314	23.830770967006252
13	29.22356500973049	19.39121393385147	25.018617535573156	26.366603520844876
14	23.91045377850992	19.232209088499534	24.650658191291857	32.20667894169869
15	25.328355129628637	26.862491425588807	21.805543228257733	26.003610216524827
16	26.042461307715183	25.6934167576647	23.109699547811285	25.154422386808832
17	24.403998537693763	25.892283043242653	24.6571397337051	25.046578685358483
18	25.109615164239102	25.03791941280004	25.34080457912537	24.511660843835486
19	25.716986985881196	24.957869228906997	24.980550508742283	24.344593276469524
20	26.089797222979517	24.18441474810337	24.4393549114907	25.286433117426416
21	26.802264707022914	24.0156245042428	24.339489829853193	24.84262095888109
22	26.307516290516215	23.849879859462895	24.639064214220948	25.20353963579994
23	24.802479290288467	24.071088655190646	25.225721167968977	25.90071088655191
24	24.945430530347675	24.657174467775075	24.875804881316178	25.521590120561072
25	25.104333811793143	24.2562084713826	24.84939679398633	25.790060922837927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	285.0
3	461.0
4	461.0
5	461.0
6	1057.5
7	1654.0
8	1654.0
9	1654.0
10	2021.0
11	2388.0
12	2388.0
13	2388.0
14	3421.0
15	4454.0
16	4454.0
17	4454.0
18	7357.0
19	10260.0
20	10260.0
21	10260.0
22	16013.0
23	21766.0
24	21766.0
25	21766.0
26	31855.0
27	41944.0
28	41944.0
29	41944.0
30	52611.0
31	63278.0
32	63278.0
33	63278.0
34	80345.5
35	97413.0
36	97413.0
37	97413.0
38	115480.5
39	133548.0
40	133548.0
41	133548.0
42	155235.5
43	176923.0
44	176923.0
45	176923.0
46	196849.0
47	216775.0
48	216775.0
49	216775.0
50	225519.5
51	234264.0
52	234264.0
53	234264.0
54	221749.0
55	209234.0
56	209234.0
57	209234.0
58	193377.0
59	177520.0
60	177520.0
61	177520.0
62	155952.5
63	134385.0
64	134385.0
65	134385.0
66	110193.5
67	86002.0
68	86002.0
69	86002.0
70	65604.5
71	45207.0
72	45207.0
73	45207.0
74	34125.5
75	23044.0
76	23044.0
77	23044.0
78	18254.5
79	13465.0
80	13465.0
81	13465.0
82	9364.0
83	5263.0
84	5263.0
85	5263.0
86	3873.5
87	2484.0
88	2484.0
89	2484.0
90	1631.0
91	778.0
92	778.0
93	778.0
94	481.5
95	185.0
96	185.0
97	185.0
98	433.0
99	681.0
100	681.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.109223151363235E-4
2	2.3481275150647057E-4
3	5.283286908895588E-4
4	0.011388418448063823
5	0.03404784896843823
6	0.06281241102798087
7	0.10543092542640528
8	0.10607666049304808
9	0.11511695142604719
10	0.13067329621335086
11	0.1364262086252594
12	0.12873609101342248
13	0.1251551965529488
14	0.1131797462261188
15	0.12926441970431204
16	0.11640842155933279
17	0.1211046765894622
18	0.10860089757174264
19	0.09633193130552954
20	0.08905273600882896
21	0.09057901889362102
22	0.10143910865079528
23	0.08130391520911544
24	0.08958106469971851
25	0.08781996906341999
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1703485.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.838241529775814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56967998605006	44.43031009508031
2	11.802411011558895	13.180517533942226
3	3.5753427263056565	5.989225521095472
4	1.5692702635874651	3.505011680147673
5	0.8613410450963264	2.4047884657799097
6	0.5603942419296557	1.8774857419658242
7	0.3663282939734321	1.4318589430655453
8	0.2677515210787499	1.1960619283168077
9	0.18980963500248138	0.9538772619552429
>10	1.0984336842495448	10.994450013828102
>50	0.07067989173222529	2.7078876157872704
>100	0.05938190451333181	6.910095273961304
>500	0.006750010057631989	2.5547342274641114
>1k	0.002425784864461496	1.863695697610172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2886	0.1694174002119185	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1941	0.11394288766851483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	5.870318787661764E-5
6	0.0	0.0	0.0	0.0	5.870318787661764E-5
7	0.0	0.0	0.0	0.0	5.870318787661764E-5
8	0.0	0.0	0.0	0.0	5.870318787661764E-5
9	0.0	0.0	0.0	0.0	5.870318787661764E-5
10	0.0	0.0	0.0	0.0	5.870318787661764E-5
11	0.0	0.0	0.0	0.0	5.870318787661764E-5
12	0.0	0.0	0.0	0.0	5.283286908895588E-4
13	0.0	0.0	0.0	0.0	6.457350666427941E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	160	0.0	14.25097	16
GTATACG	40	0.005290385	14.245526	1
GGTATCA	690	0.0	14.176707	1
CGAGCCG	285	0.0	13.667195	15
GTAATAC	85	2.7030728E-7	13.410313	3
ACGAACG	175	0.0	13.029075	15
GTATCAA	1870	0.0	12.39183	1
CCGATAA	195	0.0	12.180316	9
TTAGGTT	55	0.0030697887	12.09031	4
AGGCCCG	340	0.0	12.016231	10
GTTCTAA	95	1.0439053E-6	11.996232	1
AGTCCGA	80	2.8630133E-5	11.876507	10
TAATTCC	280	0.0	11.874413	4
GATAACG	195	0.0	11.69379	11
TAACGAA	195	0.0	11.6927595	13
AATTCCG	195	0.0	11.691729	5
ACCGTCG	285	0.0	11.66746	8
CGATAAC	205	0.0	11.586836	10
ATTCCGA	205	0.0	11.586154	6
CGTTATT	165	7.2759576E-12	11.512889	2
>>END_MODULE