FastQCFastQC Report
Thu 2 Feb 2017
SRR4062122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703485
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.028.9467751
GTATCAA22150.026.126951
CGCAATA3650.021.09625636
ATACCGT5200.017.7684976
CGTCGTA4350.017.70093710
GGCGTTA4750.017.6013442
ATACGAA4300.017.39667140
GCGTTAT4850.017.23791943
TCAACGC32850.017.2108574
TACGAAT4350.017.1967141
GTATAGG2055.456968E-1217.1740551
CAATACG4500.017.11191238
CAACGCA33350.017.0847515
CGAATGC4250.017.0831543
CTAGCGG4250.017.08164829
ATCAACG33350.016.9528223
TCTAGCG4300.016.88302228
CGTTATT5100.016.82431644
AATACGA4750.016.67495239
ACCGTCG4750.016.6729958
Produced by FastQC (version 0.11.5)