FastQCFastQC Report
Thu 2 Feb 2017
SRR4062121_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062121_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657829
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49330.13486141643034708No Hit
GCGCAAGACGGACCAGAGCGAAAGC37200.1016996693940586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.013.940731
GTATCAA37750.012.3509051
CGGACCA7900.012.0269899
CGCAAGA7600.011.4978212
AAGACGG8700.011.466645
TCGCGTA4000.011.4015869
GTCCTAA4500.011.3950321
CAAGACG9000.011.2946224
CGCATCG4550.011.27505813
CGTCGTA5700.011.16791310
GACGGAC8350.011.1515577
CCGTCGT5650.011.0988889
CGGTCCA5550.010.95617810
ACGGACC8650.010.8743448
GTCCTAC7100.010.8333051
GCGTAAC4300.010.82723411
GCAAGAC10450.010.8174653
CGCGTAA4150.010.76023810
AACCGCG1701.382432E-1010.6194147
CGGTTCT5100.010.618978512
Produced by FastQC (version 0.11.5)