##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062121_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3657829
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239364114615526	32.0	32.0	32.0	32.0	32.0
2	30.81169868793757	32.0	32.0	32.0	32.0	32.0
3	30.832289590355373	32.0	32.0	32.0	32.0	32.0
4	30.83782347397869	32.0	32.0	32.0	32.0	32.0
5	30.765648148122835	32.0	32.0	32.0	32.0	32.0
6	34.384641272186315	36.0	36.0	36.0	32.0	36.0
7	34.32936176076028	36.0	36.0	36.0	32.0	36.0
8	34.288958833231405	36.0	36.0	36.0	32.0	36.0
9	34.41780493292606	36.0	36.0	36.0	32.0	36.0
10	34.13916724920711	36.0	36.0	36.0	32.0	36.0
11	34.38931316909566	36.0	36.0	36.0	32.0	36.0
12	34.23292504925736	36.0	36.0	36.0	32.0	36.0
13	34.30838893780983	36.0	36.0	36.0	32.0	36.0
14	34.22666013091372	36.0	36.0	36.0	32.0	36.0
15	34.17267400963796	36.0	36.0	36.0	32.0	36.0
16	34.17444281840403	36.0	36.0	36.0	32.0	36.0
17	34.11580257032245	36.0	36.0	36.0	32.0	36.0
18	34.117492917246814	36.0	36.0	36.0	32.0	36.0
19	34.11525989869948	36.0	36.0	36.0	32.0	36.0
20	34.09987837047604	36.0	36.0	36.0	32.0	36.0
21	34.0785217132895	36.0	36.0	36.0	32.0	36.0
22	34.05149557292044	36.0	36.0	36.0	32.0	36.0
23	34.01712436529974	36.0	36.0	36.0	32.0	36.0
24	33.998864900464184	36.0	36.0	36.0	32.0	36.0
25	33.63923955985914	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	48.0
5	281.0
6	793.0
7	155.0
8	597.0
9	525.0
10	282.0
11	63.0
12	125.0
13	183.0
14	386.0
15	634.0
16	1136.0
17	1592.0
18	2227.0
19	3047.0
20	4673.0
21	6706.0
22	10092.0
23	14681.0
24	21101.0
25	30380.0
26	42127.0
27	53951.0
28	72058.0
29	96358.0
30	127173.0
31	175092.0
32	249014.0
33	352290.0
34	770186.0
35	1619867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49944843669051	17.015980983026612	11.512158314389719	25.97241226589316
2	16.98948171890267	18.927656949955466	37.345174259436355	26.737687071705512
3	19.669501244598877	22.06563772535715	27.612762298701	30.652098731342974
4	13.091231919086752	15.205821108051296	34.48623193002334	37.21671504283861
5	15.37894173185968	35.88059655776521	33.111942367973555	15.628519342401557
6	35.89910457609133	34.14012578554416	16.083791741244294	13.876977897120216
7	31.626118010696995	29.589019801457226	19.42796824125741	19.356893946588364
8	28.467175059416487	33.12235143688825	18.4813737113656	19.929099792329662
9	26.916249966128397	14.667950183700409	18.216763473302198	40.199036376868996
10	16.40516996418781	26.624045359553634	30.849949602320976	26.12083507393758
11	37.648071771468	20.963176604735395	21.479396775814088	19.90935484798252
12	24.801261675936424	23.329288166532024	28.238868696513663	23.63058146101789
13	29.817746827404218	19.322039001850452	24.50908254771212	26.351131623033208
14	23.44432717793648	19.491865000947012	24.61427043384107	32.44953738727544
15	25.339185167178048	27.209685967644088	21.620795910724492	25.83033295445337
16	25.9318756743648	25.800328348257175	23.339687165896887	24.92810881148113
17	24.242715390405802	26.12854307808116	24.657113529049475	24.97162800246356
18	24.872930562926296	25.14900603797071	25.660212315642916	24.317851083460077
19	25.731045042012667	24.976636824742663	24.8294111932528	24.46290693999187
20	25.864951134984558	24.522562187795444	24.589986742209167	25.022499935010828
21	26.5269589211725	24.230055882003654	24.32897540173912	24.914009795084727
22	25.94365156470218	24.332015161259424	24.72155553427019	25.0027777397682
23	24.706629466771403	24.520575268636268	25.06062493963499	25.71217032495734
24	24.903289220028277	24.848506739585925	24.986338584734842	25.261865455650955
25	25.022916688329204	24.63490637130453	24.866700122751357	25.4754768176149
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	241.0
1	241.0
2	591.0
3	941.0
4	941.0
5	941.0
6	1911.0
7	2881.0
8	2881.0
9	2881.0
10	3695.5
11	4510.0
12	4510.0
13	4510.0
14	6350.0
15	8190.0
16	8190.0
17	8190.0
18	13900.0
19	19610.0
20	19610.0
21	19610.0
22	32229.5
23	44849.0
24	44849.0
25	44849.0
26	67705.0
27	90561.0
28	90561.0
29	90561.0
30	116858.0
31	143155.0
32	143155.0
33	143155.0
34	178951.5
35	214748.0
36	214748.0
37	214748.0
38	251001.5
39	287255.0
40	287255.0
41	287255.0
42	330880.0
43	374505.0
44	374505.0
45	374505.0
46	416841.5
47	459178.0
48	459178.0
49	459178.0
50	477288.0
51	495398.0
52	495398.0
53	495398.0
54	473177.0
55	450956.0
56	450956.0
57	450956.0
58	417658.5
59	384361.0
60	384361.0
61	384361.0
62	338882.5
63	293404.0
64	293404.0
65	293404.0
66	241313.5
67	189223.0
68	189223.0
69	189223.0
70	143637.5
71	98052.0
72	98052.0
73	98052.0
74	73391.0
75	48730.0
76	48730.0
77	48730.0
78	38618.0
79	28506.0
80	28506.0
81	28506.0
82	19614.5
83	10723.0
84	10723.0
85	10723.0
86	7688.5
87	4654.0
88	4654.0
89	4654.0
90	3082.0
91	1510.0
92	1510.0
93	1510.0
94	934.5
95	359.0
96	359.0
97	359.0
98	844.0
99	1329.0
100	1329.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012028993154135964
2	1.9137034563398124E-4
3	6.561268993165072E-4
4	0.010416014526649552
5	0.03420061462687293
6	0.06129318784448371
7	0.10678465286376154
8	0.10828827700802854
9	0.11826687360180041
10	0.13349448539010433
11	0.1400830930040743
12	0.13423262815183543
13	0.12764402053786547
14	0.11572438186694894
15	0.1355175433296636
16	0.12004388395411596
17	0.1251835446654286
18	0.11258044047439068
19	0.09770823075654984
20	0.09224050659557896
21	0.09139300935062847
22	0.10331264802154502
23	0.0814690899984663
24	0.09229518383718868
25	0.08986204658555662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3657829.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.714902400852246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.19623001411598	31.175839992013827
2	16.273543502501063	14.878869058657187
3	6.415425600041509	8.798416654974798
4	3.1088064246316844	5.684751291407195
5	1.7676326689709465	4.0403577471282395
6	1.0636790674943477	2.917559085380018
7	0.6611219994315469	2.115618937534862
8	0.44812151663179356	1.6388665117235461
9	0.3479053163342725	1.4314011822863004
>10	1.5599268505268105	12.129516982750479
>50	0.082751380035535	2.605494441167903
>100	0.061140977665403766	5.896695359764115
>500	0.008601752076886276	2.7293340813526163
>1k	0.005112929542357343	3.9572786738589625
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4933	0.13486141643034708	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3720	0.1016996693940586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.467724160970893E-5
2	0.0	0.0	0.0	0.0	5.467724160970893E-5
3	0.0	0.0	0.0	2.7338620804854465E-5	5.467724160970893E-5
4	0.0	0.0	0.0	2.7338620804854465E-5	5.467724160970893E-5
5	0.0	0.0	0.0	2.7338620804854465E-5	5.467724160970893E-5
6	0.0	0.0	0.0	2.7338620804854465E-5	8.20158624145634E-5
7	0.0	0.0	0.0	2.7338620804854465E-5	8.20158624145634E-5
8	2.7338620804854465E-5	0.0	0.0	5.467724160970893E-5	8.20158624145634E-5
9	2.7338620804854465E-5	0.0	0.0	5.467724160970893E-5	8.20158624145634E-5
10	5.467724160970893E-5	0.0	0.0	5.467724160970893E-5	8.20158624145634E-5
11	8.20158624145634E-5	0.0	0.0	8.20158624145634E-5	8.20158624145634E-5
12	1.366931040242723E-4	0.0	0.0	8.20158624145634E-5	2.1870896643883572E-4
13	1.366931040242723E-4	0.0	0.0	8.20158624145634E-5	3.280634496582536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1410	0.0	13.94073	1
GTATCAA	3775	0.0	12.350905	1
CGGACCA	790	0.0	12.026989	9
CGCAAGA	760	0.0	11.497821	2
AAGACGG	870	0.0	11.46664	5
TCGCGTA	400	0.0	11.401586	9
GTCCTAA	450	0.0	11.395032	1
CAAGACG	900	0.0	11.294622	4
CGCATCG	455	0.0	11.275058	13
CGTCGTA	570	0.0	11.167913	10
GACGGAC	835	0.0	11.151557	7
CCGTCGT	565	0.0	11.098888	9
CGGTCCA	555	0.0	10.956178	10
ACGGACC	865	0.0	10.874344	8
GTCCTAC	710	0.0	10.833305	1
GCGTAAC	430	0.0	10.827234	11
GCAAGAC	1045	0.0	10.817465	3
CGCGTAA	415	0.0	10.760238	10
AACCGCG	170	1.382432E-10	10.619414	7
CGGTTCT	510	0.0	10.6189785	12
>>END_MODULE