FastQCFastQC Report
Thu 2 Feb 2017
SRR4062121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657829
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40250.026.0239541
GGTATCA17800.024.6017111
CGCAATA6650.020.18057636
CAATACG7500.018.48112538
CTAGCGG7550.018.3574829
AATACGA7450.018.31009139
TCTAGCG7650.017.83017528
TACCGTC8950.017.6974667
ATACCGT8600.017.6503076
ACCGTCG9000.017.3547158
CGTCGTA8850.017.15171210
ATACGAA8100.017.11262140
GCGCAAT7850.017.09471335
TACGAAT8100.016.84099241
ATCAACG61750.016.815383
TCAACGC61900.016.7390924
AACGCAG63300.016.5078896
CGAATGC8200.016.36729843
CCGTCGT9550.016.3552289
CAACGCA63750.016.253335
Produced by FastQC (version 0.11.5)