##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3657829
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23001075227956	32.0	32.0	32.0	32.0	32.0
2	31.36727304638899	32.0	32.0	32.0	32.0	32.0
3	31.520279925606147	32.0	32.0	32.0	32.0	32.0
4	31.609678855955266	32.0	32.0	32.0	32.0	32.0
5	31.570151857836986	32.0	32.0	32.0	32.0	32.0
6	35.2011400751648	36.0	36.0	36.0	36.0	36.0
7	35.2019279742164	36.0	36.0	36.0	36.0	36.0
8	35.147547083256214	36.0	36.0	36.0	36.0	36.0
9	35.244790010686664	36.0	36.0	36.0	36.0	36.0
10	35.105916378267	36.0	36.0	36.0	36.0	36.0
11	35.24529194776464	36.0	36.0	36.0	36.0	36.0
12	35.155550464496834	36.0	36.0	36.0	36.0	36.0
13	35.198892567148434	36.0	36.0	36.0	36.0	36.0
14	35.156128129554446	36.0	36.0	36.0	36.0	36.0
15	35.13158132870618	36.0	36.0	36.0	36.0	36.0
16	35.147754310001915	36.0	36.0	36.0	36.0	36.0
17	35.12483361031913	36.0	36.0	36.0	36.0	36.0
18	35.12112758688282	36.0	36.0	36.0	36.0	36.0
19	35.11308620495928	36.0	36.0	36.0	36.0	36.0
20	35.111858154112724	36.0	36.0	36.0	36.0	36.0
21	35.10319345163484	36.0	36.0	36.0	36.0	36.0
22	35.08825070827532	36.0	36.0	36.0	36.0	36.0
23	35.051432420706384	36.0	36.0	36.0	36.0	36.0
24	35.03963689937392	36.0	36.0	36.0	36.0	36.0
25	35.01624242139258	36.0	36.0	36.0	36.0	36.0
26	34.973593079392174	36.0	36.0	36.0	36.0	36.0
27	34.96017282382528	36.0	36.0	36.0	36.0	36.0
28	34.93989686231915	36.0	36.0	36.0	32.0	36.0
29	34.9153708388227	36.0	36.0	36.0	32.0	36.0
30	34.894338144292696	36.0	36.0	36.0	32.0	36.0
31	34.894736467997824	36.0	36.0	36.0	32.0	36.0
32	34.867478222738136	36.0	36.0	36.0	32.0	36.0
33	34.846063334289276	36.0	36.0	36.0	32.0	36.0
34	34.83764057860551	36.0	36.0	36.0	32.0	36.0
35	34.821889705615	36.0	36.0	36.0	32.0	36.0
36	34.804630287528475	36.0	36.0	36.0	32.0	36.0
37	34.78587517349772	36.0	36.0	36.0	32.0	36.0
38	34.76594149152407	36.0	36.0	36.0	32.0	36.0
39	34.747625435743444	36.0	36.0	36.0	32.0	36.0
40	34.73958842799923	36.0	36.0	36.0	32.0	36.0
41	34.71030876511723	36.0	36.0	36.0	32.0	36.0
42	34.67945357751825	36.0	36.0	36.0	32.0	36.0
43	34.6800837327278	36.0	36.0	36.0	32.0	36.0
44	34.62103695935485	36.0	36.0	36.0	32.0	36.0
45	34.61456071347239	36.0	36.0	36.0	32.0	36.0
46	34.591471607885445	36.0	36.0	36.0	32.0	36.0
47	34.5677949953374	36.0	36.0	36.0	32.0	36.0
48	34.542754185611194	36.0	36.0	36.0	32.0	36.0
49	34.529149394353865	36.0	36.0	36.0	32.0	36.0
50	34.09775470641192	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	31.0
21	83.0
22	246.0
23	750.0
24	1933.0
25	4452.0
26	9352.0
27	18051.0
28	30896.0
29	49359.0
30	73575.0
31	109441.0
32	167259.0
33	284288.0
34	642051.0
35	2266051.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.069350276653445	17.5508191583708	11.975034600481687	26.404795964494067
2	16.375146917259645	19.43400595624921	36.96535308943861	27.22549403705254
3	19.034288182578692	22.54938734000033	27.950391216626297	30.46593326079468
4	12.828811844402788	15.626263556880323	34.669198587468145	36.87572601124875
5	15.0393621569959	36.29596580265666	32.87355228293949	15.791119757407948
6	36.02621012553095	34.3431598521853	15.923706532546818	13.706923489736928
7	31.726387428171194	29.55105337072892	19.461188590281285	19.261370610818602
8	28.594502367387868	33.239826137307126	18.33579426484945	19.82987723045555
9	27.009737196572065	14.380305913699083	18.14836068061137	40.46159620911748
10	16.369782589380304	26.634325042887507	30.906153246806507	26.089739120925685
11	37.9054898411052	20.78363969447451	21.488319984340436	19.82255048007985
12	24.864229574427892	23.220932416468894	28.264033119098787	23.650804890004427
13	29.90096037841025	19.18832181602803	24.595955688469857	26.31476211709186
14	23.613569688468214	19.29723888131457	24.586879266362644	32.50231216385457
15	25.4189848678	27.13360848743886	21.574354624013313	25.873052020747828
16	26.10001733279458	25.656117355595132	23.311241766239107	24.93262354537119
17	24.445839321630398	25.996048475748868	24.634749191391943	24.92336301122879
18	25.0968538988564	25.047453011062025	25.61060126102122	24.245091829060353
19	25.92054629140572	24.764149653838288	24.815054179693526	24.50024987506247
20	26.020130033807042	24.363240598630114	24.596549090195303	25.020080277367544
21	26.69665531111487	24.16386878664913	24.30518211758942	24.834293784646576
22	26.044000416640582	24.247142225620717	24.707633954457688	25.00122340328102
23	24.832024679119773	24.354090910209308	25.047999783478126	25.765884627192797
24	25.115233578326574	24.706133871071806	24.965826340947043	25.212806209654577
25	25.216925595867046	24.389602443640626	24.909939551443333	25.48353240904899
26	24.905442183379968	25.314566209608742	25.190831138261505	24.58916046874979
27	25.387847451088994	24.648253316117234	24.798096788330305	25.16580244446346
28	25.003355859741195	24.654729192371004	25.177450481589798	25.164464466298007
29	24.863935200677126	24.828285512447863	25.123789714955592	25.18398957191942
30	24.8875001913727	24.94641564400741	25.248511120394205	24.917573044225687
31	25.493566784305266	24.653419953398384	24.50628285262259	25.346730409673757
32	25.242707298729485	24.71602473726701	24.558060977073385	25.483206986930124
33	24.876531683753797	24.49217717324057	25.016915808619395	25.614375334386235
34	25.384288580178566	24.698903547981597	25.046271007857733	24.8705368639821
35	25.651597304295265	24.533772720848134	25.065291639635532	24.749338335221072
36	24.760950772918545	25.053036996859333	24.8719457251087	25.314066505113423
37	25.714056846782267	24.579592658024225	24.697451611121775	25.008898884071733
38	25.05210057659885	24.671464959132862	25.001660821213896	25.274773643054395
39	25.707575991612458	24.478450874226482	24.49209293551734	25.32188019864372
40	25.720879802242973	24.58151326117017	24.962997105928064	24.73460983065879
41	24.9432101668086	24.990315177081214	25.25755341005554	24.808921246054645
42	25.817484600097384	25.074385131667764	24.651408977286803	24.456721290948046
43	25.38695398983506	24.35484885186471	24.998209242495907	25.25998791580432
44	25.012063740292618	24.979447214130364	24.663288363719378	25.345200681857637
45	25.095810475424457	25.04769335836152	24.69187274553536	25.164623420678662
46	24.981525472429826	24.75460933653276	24.737330903052403	25.526534287985008
47	25.24313914825979	24.696277689594403	24.99337029926879	25.067212862877007
48	25.627692277408325	25.085874012079774	24.264589286554006	25.021844423957894
49	25.071992060573667	25.17355875763288	24.437056670936204	25.317392510857246
50	25.265859287728397	25.2783259271653	24.395709726329926	25.060105058776376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	71.5
2	62.0
3	181.5
4	301.0
5	326.0
6	351.0
7	297.5
8	244.0
9	323.0
10	402.0
11	753.0
12	1104.0
13	2025.0
14	2946.0
15	3868.5
16	4791.0
17	4958.0
18	5125.0
19	5269.5
20	5414.0
21	6475.5
22	7537.0
23	9493.0
24	11449.0
25	14988.5
26	18528.0
27	24087.0
28	29646.0
29	35924.0
30	42202.0
31	49172.5
32	56143.0
33	65267.0
34	74391.0
35	87488.0
36	100585.0
37	115643.0
38	130701.0
39	142043.5
40	153386.0
41	166401.0
42	179416.0
43	187151.5
44	194887.0
45	211512.5
46	228138.0
47	243051.5
48	257965.0
49	271869.0
50	285773.0
51	281515.5
52	277258.0
53	276980.0
54	276702.0
55	276939.0
56	277176.0
57	266057.0
58	254938.0
59	235816.0
60	216694.0
61	191955.0
62	167216.0
63	145436.5
64	123657.0
65	106150.0
66	88643.0
67	76268.5
68	63894.0
69	55304.5
70	46715.0
71	36314.0
72	25913.0
73	22564.0
74	19215.0
75	14572.5
76	9930.0
77	8603.5
78	7277.0
79	6006.5
80	4736.0
81	3699.5
82	2663.0
83	2281.0
84	1899.0
85	1477.5
86	1056.0
87	729.5
88	403.0
89	261.0
90	119.0
91	86.0
92	53.0
93	46.0
94	39.0
95	34.0
96	29.0
97	28.5
98	28.0
99	18.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029744419435681655
2	0.004319502087167006
3	2.460475872436902E-4
4	0.0
5	2.733862080485446E-5
6	1.640317248291268E-4
7	0.0
8	0.0
9	0.0
10	1.0935448321941785E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.287882785116527E-4
17	0.0
18	0.0
19	2.733862080485446E-5
20	3.007248288533991E-4
21	0.0
22	0.0
23	0.0
24	0.001120883452999033
25	2.187089664388357E-4
26	3.827406912679625E-4
27	3.280634496582536E-4
28	0.0013122537986330143
29	3.5540207046310806E-4
30	0.001230237936218451
31	4.374179328776714E-4
32	7.65481382535925E-4
33	2.187089664388357E-4
34	8.474972449504884E-4
35	2.733862080485446E-4
36	1.366931040242723E-4
37	0.0018316875939252489
38	0.0
39	6.561268993165072E-4
40	1.9137034563398124E-4
41	0.0012028993154135964
42	0.004811597261654386
43	0.00434684070797186
44	0.004756920020044676
45	0.002378460010022338
46	0.002460475872436902
47	0.00128491517782816
48	0.003964100016703897
49	0.0036633751878504977
50	0.0018316875939252489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3657829.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.8748066091174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.71242862055709	31.7400699662228
2	16.698220281167462	15.654516927923334
3	6.574128677223061	9.244830311048512
4	3.1731126903919313	5.94956174844232
5	1.7342514657214467	4.06463510336356
6	1.0062182962596777	2.8299772826236795
7	0.6733177716468245	2.209314823269874
8	0.463997910635935	1.739984986247519
9	0.3389564917210983	1.4299667998498249
>10	1.490986761438297	11.925921282664877
>50	0.06889043934097984	2.2135838336646474
>100	0.05328407072036445	5.064449395298817
>500	0.00792250303991244	2.577718770523594
>1k	0.0042840201361153815	3.355468768856652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7338620804854465E-5
2	0.0	0.0	0.0	0.0	2.7338620804854465E-5
3	0.0	0.0	0.0	0.0	2.7338620804854465E-5
4	0.0	0.0	0.0	0.0	2.7338620804854465E-5
5	0.0	0.0	0.0	0.0	2.7338620804854465E-5
6	5.467724160970893E-5	0.0	0.0	0.0	2.7338620804854465E-5
7	1.640317248291268E-4	0.0	0.0	0.0	8.20158624145634E-5
8	1.9137034563398124E-4	0.0	0.0	0.0	8.20158624145634E-5
9	1.9137034563398124E-4	0.0	0.0	0.0	8.20158624145634E-5
10	1.9137034563398124E-4	0.0	0.0	2.7338620804854465E-5	8.20158624145634E-5
11	2.1870896643883572E-4	0.0	0.0	2.7338620804854465E-5	8.20158624145634E-5
12	2.1870896643883572E-4	0.0	0.0	2.7338620804854465E-5	2.460475872436902E-4
13	2.1870896643883572E-4	0.0	0.0	5.467724160970893E-5	2.460475872436902E-4
14	2.733862080485446E-4	0.0	0.0	1.0935448321941786E-4	2.460475872436902E-4
15	2.733862080485446E-4	0.0	0.0	1.9137034563398124E-4	3.007248288533991E-4
16	3.280634496582536E-4	0.0	0.0	3.007248288533991E-4	3.007248288533991E-4
17	3.280634496582536E-4	0.0	0.0	4.1007931207281696E-4	3.007248288533991E-4
18	3.280634496582536E-4	0.0	0.0	6.287882785116527E-4	3.007248288533991E-4
19	3.280634496582536E-4	0.0	0.0	9.295131073650518E-4	3.007248288533991E-4
20	3.280634496582536E-4	0.0	0.0	0.0012849151778281597	4.647565536825259E-4
21	3.280634496582536E-4	0.0	0.0	0.00153096276507185	5.467724160970892E-4
22	3.5540207046310806E-4	0.0	0.0	0.0017496717315106857	5.741110369019437E-4
23	3.5540207046310806E-4	0.0	0.0	0.0022144282851932116	5.741110369019437E-4
24	5.467724160970892E-4	0.0	0.0	0.0027885393220951553	6.014496577067982E-4
25	5.741110369019437E-4	0.0	0.0	0.0037453910502650615	6.014496577067982E-4
26	6.014496577067982E-4	0.0	0.0	0.004510872432800987	6.561268993165072E-4
27	6.014496577067982E-4	0.0	0.0	0.004975628986483512	6.561268993165072E-4
28	6.287882785116527E-4	0.0	0.0	0.006096512439482546	6.561268993165072E-4
29	7.381427617310705E-4	0.0	0.0	0.0073814276173107055	6.561268993165072E-4
30	7.65481382535925E-4	0.0	0.0	0.009869242110552462	6.561268993165072E-4
31	7.65481382535925E-4	0.0	0.0	0.014188744197719467	6.561268993165072E-4
32	7.65481382535925E-4	0.0	0.0	0.020531304224445702	6.561268993165072E-4
33	7.928200033407794E-4	0.0	0.0	0.025944351143806887	6.561268993165072E-4
34	7.928200033407794E-4	0.0	0.0	0.032450942895362246	6.834655201213616E-4
35	9.021744865601974E-4	0.0	0.0	0.040133095341526356	6.834655201213616E-4
36	9.021744865601974E-4	0.0	0.0	0.0498929829688594	6.834655201213616E-4
37	9.021744865601974E-4	0.0	0.0	0.06372632509611575	6.834655201213616E-4
38	9.568517281699062E-4	0.0	0.0	0.08258997345146533	7.381427617310705E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4025	0.0	26.023954	1
GGTATCA	1780	0.0	24.601711	1
CGCAATA	665	0.0	20.180576	36
CAATACG	750	0.0	18.481125	38
CTAGCGG	755	0.0	18.35748	29
AATACGA	745	0.0	18.310091	39
TCTAGCG	765	0.0	17.830175	28
TACCGTC	895	0.0	17.697466	7
ATACCGT	860	0.0	17.650307	6
ACCGTCG	900	0.0	17.354715	8
CGTCGTA	885	0.0	17.151712	10
ATACGAA	810	0.0	17.112621	40
GCGCAAT	785	0.0	17.094713	35
TACGAAT	810	0.0	16.840992	41
ATCAACG	6175	0.0	16.81538	3
TCAACGC	6190	0.0	16.739092	4
AACGCAG	6330	0.0	16.507889	6
CGAATGC	820	0.0	16.367298	43
CCGTCGT	955	0.0	16.355228	9
CAACGCA	6375	0.0	16.25333	5
>>END_MODULE