FastQCFastQC Report
Thu 2 Feb 2017
SRR4062120_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062120_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276696
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10510.37983924595946456No Hit
TATCAACGCAGAGTACTTTTTTTTT6610.23889033451874983No Hit
CATTTACACCTACTACCCAACTATC5910.21359181195246768No Hit
GTATAGTAGGGGTGAAATGGAATTT4560.16480180414606643No Hit
GTATTGGAATTAGTGAAATTGGAGT4500.16263335935467083No Hit
GTATAGGGGTCCTAGGAAGATAATA4440.16046491456327522No Hit
GGTATCAACGCAGAGTACTTTTTTT4340.1568508399109492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4160.15034550553676235No Hit
GTGTAAATGTATGTGGTAAAAGGCC3980.14384017116257553No Hit
GATATAGGCTTACTAGGAGGGTGAA3730.13480498453176049No Hit
ATCCTATTCCCATCCTCAAAACGCC3540.12793824269234105No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3290.11890305606152601No Hit
ATTAATAAGTGTCCTGCAGTAATGT3270.11818024113106079No Hit
GTGTTGGAAAGAATGGAGACGGTTG2770.10010986786943071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG250.006015167619.00253515
CCAGCAA359.891912E-519.00253313
TGGCACT250.00602146218.9990964
AGCATTC307.7032496E-418.9990966
GGCGAGG402.7480203E-416.62721619
GGGGAGC350.00216220316.28788619
CCCAGCA350.00216220316.28788612
CAATACT651.8976607E-716.0761594
GACGTGA551.1221302E-515.5447147
TTCCCAG508.6628104E-515.20202710
AAATTGG755.776201E-815.20202716
TATACAA508.676204E-515.1992775
AATTGGA755.7891157E-815.19927517
CTATACA456.739899E-414.7770744
CGGTCGA400.005259917614.25190110
ATACCCC400.005259917614.25190110
GGCTTCG400.005266241314.2493217
TAAGCCG400.005266241314.24932117
TACAATC400.005266241314.2493217
GTATACA400.005278907314.2441651
Produced by FastQC (version 0.11.5)