##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062120_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276696
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20476985572614	32.0	32.0	32.0	32.0	32.0
2	30.778706594963424	32.0	32.0	32.0	32.0	32.0
3	30.840279584815104	32.0	32.0	32.0	32.0	32.0
4	30.82486194234828	32.0	32.0	32.0	32.0	32.0
5	30.744643941365254	32.0	32.0	32.0	32.0	32.0
6	34.3958242981467	36.0	36.0	36.0	32.0	36.0
7	34.33845809119033	36.0	36.0	36.0	32.0	36.0
8	34.26653077745974	36.0	36.0	36.0	32.0	36.0
9	34.37183045652991	36.0	36.0	36.0	32.0	36.0
10	34.080879376644404	36.0	36.0	36.0	32.0	36.0
11	34.41183826293116	36.0	36.0	36.0	32.0	36.0
12	34.227025327435165	36.0	36.0	36.0	32.0	36.0
13	34.315606297163676	36.0	36.0	36.0	32.0	36.0
14	34.24495475178535	36.0	36.0	36.0	32.0	36.0
15	34.17864371006448	36.0	36.0	36.0	32.0	36.0
16	34.18264087662995	36.0	36.0	36.0	32.0	36.0
17	34.138603376991355	36.0	36.0	36.0	32.0	36.0
18	34.14740003469512	36.0	36.0	36.0	32.0	36.0
19	34.12752623818198	36.0	36.0	36.0	32.0	36.0
20	34.09414303061844	36.0	36.0	36.0	32.0	36.0
21	34.09278413854916	36.0	36.0	36.0	32.0	36.0
22	34.05814323300662	36.0	36.0	36.0	32.0	36.0
23	34.04580478214358	36.0	36.0	36.0	32.0	36.0
24	34.02406612310984	36.0	36.0	36.0	32.0	36.0
25	33.70180270043658	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	20.0
6	55.0
7	15.0
8	54.0
9	36.0
10	27.0
11	7.0
12	8.0
13	18.0
14	72.0
15	95.0
16	153.0
17	182.0
18	202.0
19	346.0
20	424.0
21	622.0
22	898.0
23	1200.0
24	1659.0
25	2293.0
26	3030.0
27	3941.0
28	5334.0
29	6855.0
30	9089.0
31	12476.0
32	18209.0
33	26071.0
34	58399.0
35	124902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.2618805815874	22.249368429041784	13.634704417563276	23.85404657180754
2	14.062003064735304	22.391722323416314	41.04685286379276	22.499421748055628
3	16.722082878558986	27.763883568129415	29.785250131914676	25.728783421396923
4	11.435604020659918	18.051765454276016	40.05117992127834	30.46145060378572
5	12.425526376677464	38.48912540490514	35.95919134659879	13.126156871818603
6	28.438966881477786	40.83544889737525	18.331633649402217	12.393950571744744
7	26.589120642558655	31.73356971001646	24.265996128728812	17.41131351869607
8	23.949868665745278	37.01744610953928	21.29713380174678	17.73555142296866
9	25.207061624578913	15.873819956796579	22.403904952472615	36.51521346615189
10	15.614833150578844	27.63030757037857	33.55987652310487	23.194982755937712
11	31.656038045878	25.522805087260856	24.866629508718844	17.954527358142293
12	20.925123683991472	27.547419736599245	30.791975650449317	20.73548092895997
13	26.674242040429334	22.745300976354702	27.807291176619596	22.773165806596367
14	20.631239213103843	21.634940678855333	28.90399707645716	28.82982303158367
15	23.457692627830472	30.82370993366459	22.596381770609035	23.122215667895905
16	22.334635982052394	28.816760746851934	26.84071500940802	22.007888261687654
17	19.648943791928094	30.676349478589987	27.62640845834871	22.048298271133206
18	19.82357815060984	30.020659736669764	29.13276142165039	21.023000691070003
19	22.666956081325516	27.78308371319007	28.108313436075537	21.44164676940887
20	22.279700901852543	28.271431207064328	28.23380879857903	21.215059092504095
21	22.763498236411323	27.843718911097042	27.445419191462424	21.94736366102921
22	21.987496291579532	27.863443295537593	28.660120550799935	21.48893986208294
23	21.74892839702664	29.213434374491325	28.095711779493946	20.94192544898808
24	21.85416568693771	27.778119426849518	28.594942066365935	21.772772819846836
25	21.891063786685574	28.048343745591218	28.58626016054291	21.474332307180298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	490.0
1	490.0
2	340.0
3	190.0
4	190.0
5	190.0
6	355.0
7	520.0
8	520.0
9	520.0
10	613.0
11	706.0
12	706.0
13	706.0
14	1010.5
15	1315.0
16	1315.0
17	1315.0
18	3107.0
19	4899.0
20	4899.0
21	4899.0
22	6362.5
23	7826.0
24	7826.0
25	7826.0
26	11660.5
27	15495.0
28	15495.0
29	15495.0
30	19974.5
31	24454.0
32	24454.0
33	24454.0
34	27200.0
35	29946.0
36	29946.0
37	29946.0
38	32489.5
39	35033.0
40	35033.0
41	35033.0
42	34716.0
43	34399.0
44	34399.0
45	34399.0
46	34928.5
47	35458.0
48	35458.0
49	35458.0
50	31988.5
51	28519.0
52	28519.0
53	28519.0
54	24814.0
55	21109.0
56	21109.0
57	21109.0
58	18378.0
59	15647.0
60	15647.0
61	15647.0
62	12852.5
63	10058.0
64	10058.0
65	10058.0
66	7750.0
67	5442.0
68	5442.0
69	5442.0
70	4215.0
71	2988.0
72	2988.0
73	2988.0
74	2186.0
75	1384.0
76	1384.0
77	1384.0
78	948.5
79	513.0
80	513.0
81	513.0
82	319.0
83	125.0
84	125.0
85	125.0
86	95.0
87	65.0
88	65.0
89	65.0
90	43.5
91	22.0
92	22.0
93	22.0
94	19.5
95	17.0
96	17.0
97	17.0
98	46.5
99	76.0
100	76.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0018070373261630092
2	0.0
3	7.228149304652037E-4
4	0.009035186630815047
5	0.03180385694046896
6	0.06288489895047272
7	0.10878364703501314
8	0.10914505450024575
9	0.11890305606152601
10	0.1333593546708301
11	0.14384017116257553
12	0.13986468904501692
13	0.13082950241420185
14	0.11528898140919998
15	0.13480498453176049
16	0.12143290831815423
17	0.1192644635267586
18	0.11312053661780437
19	0.0997484604041981
20	0.09577297828663948
21	0.0979414230780351
22	0.10769942463931534
23	0.08709919912105704
24	0.09360453349524388
25	0.09360453349524388
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	276696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.64295833694741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.50976668475312	15.05587359412496
2	13.85241454150841	7.38138606991066
3	7.639717851329355	6.106340532570041
4	4.67444384156267	4.981640500766184
5	3.2609875203472596	4.344117732095874
6	2.3996201844818232	3.835978835978836
7	1.8109061313076504	3.3773527625986643
8	1.3402061855670102	2.856564605198485
9	1.1421595225176342	2.738745771532657
>10	6.749864351600651	33.01457194899818
>50	0.45984807379272924	8.370558302252292
>100	0.15599565925122083	7.104547951542487
>500	0.002712967986977754	0.4524821464712175
>1k	0.001356483993488877	0.37983924595946456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1051	0.37983924595946456	No Hit
TATCAACGCAGAGTACTTTTTTTTT	661	0.23889033451874983	No Hit
CATTTACACCTACTACCCAACTATC	591	0.21359181195246768	No Hit
GTATAGTAGGGGTGAAATGGAATTT	456	0.16480180414606643	No Hit
GTATTGGAATTAGTGAAATTGGAGT	450	0.16263335935467083	No Hit
GTATAGGGGTCCTAGGAAGATAATA	444	0.16046491456327522	No Hit
GGTATCAACGCAGAGTACTTTTTTT	434	0.1568508399109492	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	416	0.15034550553676235	No Hit
GTGTAAATGTATGTGGTAAAAGGCC	398	0.14384017116257553	No Hit
GATATAGGCTTACTAGGAGGGTGAA	373	0.13480498453176049	No Hit
ATCCTATTCCCATCCTCAAAACGCC	354	0.12793824269234105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	329	0.11890305606152601	No Hit
ATTAATAAGTGTCCTGCAGTAATGT	327	0.11818024113106079	No Hit
GTGTTGGAAAGAATGGAGACGGTTG	277	0.10010986786943071	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAG	25	0.0060151676	19.002535	15
CCAGCAA	35	9.891912E-5	19.002533	13
TGGCACT	25	0.006021462	18.999096	4
AGCATTC	30	7.7032496E-4	18.999096	6
GGCGAGG	40	2.7480203E-4	16.627216	19
GGGGAGC	35	0.002162203	16.287886	19
CCCAGCA	35	0.002162203	16.287886	12
CAATACT	65	1.8976607E-7	16.076159	4
GACGTGA	55	1.1221302E-5	15.544714	7
TTCCCAG	50	8.6628104E-5	15.202027	10
AAATTGG	75	5.776201E-8	15.202027	16
TATACAA	50	8.676204E-5	15.199277	5
AATTGGA	75	5.7891157E-8	15.199275	17
CTATACA	45	6.739899E-4	14.777074	4
CGGTCGA	40	0.0052599176	14.251901	10
ATACCCC	40	0.0052599176	14.251901	10
GGCTTCG	40	0.0052662413	14.249321	7
TAAGCCG	40	0.0052662413	14.249321	17
TACAATC	40	0.0052662413	14.249321	7
GTATACA	40	0.0052789073	14.244165	1
>>END_MODULE