FastQCFastQC Report
Thu 2 Feb 2017
SRR4062120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276696
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA5470.19768988348223324No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA4710.17022291612455548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.16154913695897302No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT4320.156128024980484No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4160.15034550553676235No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4000.14456298609304075No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT3930.14203313383641253No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT3470.12540839043571284No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC3190.11528898140919998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.10372394252175672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAT207.8422966E-444.0043371
CTGGGCT250.002348531535.20347237
CGCCAGC250.002348531535.20347242
GGGCCCG300.005734692329.33622434
AGGGCCC300.005734692329.33622433
AGTAGTG300.00573976229.3309237
GTAAAGG300.00573976229.3309233
GGGGTCC1006.002665E-1126.3978316
AATAACC508.323276E-526.3978312
AGATAAT1051.10958354E-1025.14079318
TTCGTTT450.001394691124.44685628
TTAGAAT1101.9826984E-1024.00236742
CGTTTAT1101.9826984E-1024.00236736
AGGGGTC1102.0008883E-1023.998035
TCGGTTT551.5901768E-423.9980313
GTCGGTT551.5901768E-423.9980312
GCGTTTA1153.4742698E-1022.95878435
TAGGGCG1153.4742698E-1022.95878431
GAAGATA1254.0017767E-1122.8781216
TTTAATC1450.022.76086640
Produced by FastQC (version 0.11.5)