##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276696
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2545790325845	32.0	32.0	32.0	32.0	32.0
2	31.499367536935843	32.0	32.0	32.0	32.0	32.0
3	31.598855783965075	32.0	32.0	32.0	32.0	32.0
4	31.6796122820713	32.0	32.0	32.0	32.0	32.0
5	31.656988174747738	32.0	32.0	32.0	32.0	32.0
6	35.28472402925955	36.0	36.0	36.0	36.0	36.0
7	35.30713129210397	36.0	36.0	36.0	36.0	36.0
8	35.2640695926215	36.0	36.0	36.0	36.0	36.0
9	35.335631884812216	36.0	36.0	36.0	36.0	36.0
10	35.23172723855784	36.0	36.0	36.0	36.0	36.0
11	35.33898213201492	36.0	36.0	36.0	36.0	36.0
12	35.267087344956195	36.0	36.0	36.0	36.0	36.0
13	35.3071818891491	36.0	36.0	36.0	36.0	36.0
14	35.27590207303322	36.0	36.0	36.0	36.0	36.0
15	35.24889409315639	36.0	36.0	36.0	36.0	36.0
16	35.261518055916966	36.0	36.0	36.0	36.0	36.0
17	35.24142741492468	36.0	36.0	36.0	36.0	36.0
18	35.25808468499725	36.0	36.0	36.0	36.0	36.0
19	35.22512793824269	36.0	36.0	36.0	36.0	36.0
20	35.23932402347703	36.0	36.0	36.0	36.0	36.0
21	35.21668184578021	36.0	36.0	36.0	36.0	36.0
22	35.21419897649406	36.0	36.0	36.0	36.0	36.0
23	35.195608176482494	36.0	36.0	36.0	36.0	36.0
24	35.18247101512129	36.0	36.0	36.0	36.0	36.0
25	35.16181296440859	36.0	36.0	36.0	36.0	36.0
26	35.12531442449475	36.0	36.0	36.0	36.0	36.0
27	35.11483722207766	36.0	36.0	36.0	36.0	36.0
28	35.100174921213174	36.0	36.0	36.0	36.0	36.0
29	35.073239222829386	36.0	36.0	36.0	36.0	36.0
30	35.03927776332148	36.0	36.0	36.0	36.0	36.0
31	35.02708026136988	36.0	36.0	36.0	36.0	36.0
32	35.02356376673317	36.0	36.0	36.0	36.0	36.0
33	35.0096423511724	36.0	36.0	36.0	36.0	36.0
34	34.972594471911414	36.0	36.0	36.0	36.0	36.0
35	34.965084424783875	36.0	36.0	36.0	36.0	36.0
36	34.93914259692948	36.0	36.0	36.0	36.0	36.0
37	34.93346127157603	36.0	36.0	36.0	36.0	36.0
38	34.91293694162547	36.0	36.0	36.0	36.0	36.0
39	34.84477187960795	36.0	36.0	36.0	36.0	36.0
40	34.84055786856333	36.0	36.0	36.0	32.0	36.0
41	34.821059935814034	36.0	36.0	36.0	32.0	36.0
42	34.793564055859136	36.0	36.0	36.0	32.0	36.0
43	34.79154740220314	36.0	36.0	36.0	32.0	36.0
44	34.713562899355246	36.0	36.0	36.0	32.0	36.0
45	34.68215297655188	36.0	36.0	36.0	32.0	36.0
46	34.643204094023766	36.0	36.0	36.0	32.0	36.0
47	34.61059429843583	36.0	36.0	36.0	32.0	36.0
48	34.571905629282675	36.0	36.0	36.0	32.0	36.0
49	34.503173157544744	36.0	36.0	36.0	32.0	36.0
50	34.10951007604013	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	12.0
23	29.0
24	112.0
25	265.0
26	585.0
27	1150.0
28	2025.0
29	3324.0
30	5067.0
31	7776.0
32	11308.0
33	19025.0
34	42369.0
35	183644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.842571837626	22.83113277126206	14.025510130298342	24.300785260813605
2	13.840637853389136	22.687022459320943	40.6313476315428	22.840992055747115
3	16.065342705867472	28.10350747212635	30.126673774372502	25.70447604763368
4	11.159178303987046	18.492497181021772	40.12815508717148	30.220169427819698
5	12.184129875386706	38.606629658542225	35.791988319310725	13.417252146760344
6	28.374822910342033	40.816274900974356	18.284326481047792	12.524575707635819
7	26.683074565588228	31.674834475380926	24.304652036892474	17.337438922138375
8	24.153222308960014	36.74682684245526	21.515309220227252	17.584641628357474
9	25.26924856159829	15.354757567872321	22.63169688033076	36.74429699019863
10	15.50262924881187	27.41249390122698	33.680044814687655	23.404832035273497
11	31.985644895480963	25.199858328273628	24.819657674848933	17.99483910139648
12	20.613959001937143	27.49660276982681	31.210064475091798	20.679373753144244
13	26.948347645068953	22.201260589238732	28.12653598172724	22.723855783965075
14	20.461806459074218	21.29954896348339	29.14462081128748	29.094023766154915
15	23.539552432995055	30.66759186977766	22.743733194552867	23.049122502674415
16	22.294865122733974	28.581186572989854	26.9931621707578	22.130786133518374
17	19.662373145979704	30.416775088906235	27.78681296440859	22.134038800705465
18	19.757784716801112	29.792985803914767	29.45651545378321	20.99271402550091
19	22.671895046892786	27.41827644156924	28.379262364697595	21.530566146840382
20	22.408708474282243	27.920172319079423	28.583355017781248	21.087764188857086
21	22.835530690721946	27.567438633012404	27.55153670454217	22.045493971723477
22	22.045493971723477	27.595628415300546	28.824775204556623	21.534102408419347
23	21.779498077312283	28.837063058374536	28.340489201144937	21.042949663168244
24	21.837763602522635	27.63692874826072	28.668389381810293	21.85691826740635
25	21.77046289068147	27.731517622228	29.05354613004886	21.444473357041662
26	21.076636729973437	28.408897883951646	29.466379949041364	21.048085437033556
27	20.875768899940006	28.987256680665286	28.493570514720233	21.64340390467448
28	21.127101614791897	27.021381174735808	29.549462940742778	22.302054269729517
29	20.80536912751678	27.804100573559865	28.492227848192762	22.898302450730593
30	20.724562779418196	28.09126426229982	28.33738719365646	22.846785764625523
31	22.663023639918613	27.34691517313412	28.700039393840825	21.29002179310644
32	20.78708740074668	28.41509120282192	28.96407906292579	21.833742333505608
33	20.917255461790056	29.31964798785667	28.54840166970852	21.214694880644753
34	21.656414667466585	26.796388790505034	30.09208728776193	21.455109254266446
35	21.205944429988147	28.633229247983344	28.377714170063896	21.783112151964612
36	21.235656589385425	28.184824445689298	28.37456405066951	22.20495491425577
37	22.855098286536244	27.07099255125754	28.22643309684811	21.84747606535811
38	21.5579553011247	28.142437910197472	29.06980946598433	21.229797322693496
39	21.642632549062128	28.1914778271712	28.392786150565613	21.773103473201054
40	21.650114204759014	27.602133749674735	29.220877786451556	21.526874259114695
41	22.32229338036517	27.75107339568907	28.681711072239167	21.24492215170659
42	21.356647703862194	28.248313948865484	28.583717047007035	21.811321300265288
43	21.192823685884463	28.194211530677155	29.000896316428616	21.612068467009774
44	20.197624744294977	28.317659729802013	29.194827349414137	22.289888176488873
45	21.790089992410277	28.13762694712494	28.243521630705843	21.828761429758934
46	21.85052531904051	27.371164014471123	28.226998543491067	22.5513121229973
47	21.14460226245979	28.865155950702952	28.651198091727203	21.339043695110053
48	20.78919501096907	28.842338093224473	27.771814360631325	22.596652535175128
49	21.7664258375108	27.856066328614336	28.253633219243685	22.123874614631184
50	20.727454091387145	28.152704641639954	28.371721523287714	22.748119743685194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	165.5
2	29.0
3	66.5
4	104.0
5	103.5
6	103.0
7	79.5
8	56.0
9	82.5
10	109.0
11	188.0
12	267.0
13	462.0
14	657.0
15	859.0
16	1061.0
17	1088.5
18	1116.0
19	1125.0
20	1134.0
21	1322.5
22	1511.0
23	1873.5
24	2236.0
25	3059.0
26	3882.0
27	5234.5
28	6587.0
29	7552.5
30	8518.0
31	10087.0
32	11656.0
33	13715.0
34	15774.0
35	17671.0
36	19568.0
37	19822.0
38	20076.0
39	21915.0
40	23754.0
41	23726.5
42	23699.0
43	21191.5
44	18684.0
45	17456.5
46	16229.0
47	15606.0
48	14983.0
49	14537.0
50	14091.0
51	13594.0
52	13097.0
53	12496.0
54	11895.0
55	11203.0
56	10511.0
57	9920.5
58	9330.0
59	8737.5
60	8145.0
61	7293.0
62	6441.0
63	5289.0
64	4137.0
65	3350.5
66	2564.0
67	2191.5
68	1819.0
69	1415.0
70	1011.0
71	860.0
72	709.0
73	529.5
74	350.0
75	310.0
76	270.0
77	198.0
78	126.0
79	87.0
80	48.0
81	33.0
82	18.0
83	11.5
84	5.0
85	6.0
86	7.0
87	7.5
88	8.0
89	9.0
90	10.0
91	7.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03614074652326019
2	0.006505334374186833
3	3.6140746523260185E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	3.6140746523260185E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	3.6140746523260185E-4
20	0.0
21	0.0
22	0.0
23	0.0
24	3.6140746523260185E-4
25	0.0
26	3.6140746523260185E-4
27	7.228149304652037E-4
28	0.0014456298609304074
29	0.0010842223956978054
30	0.0018070373261630092
31	0.0010842223956978054
32	0.0018070373261630092
33	3.6140746523260185E-4
34	7.228149304652037E-4
35	0.0
36	3.6140746523260185E-4
37	0.0018070373261630092
38	0.0
39	0.0021684447913956108
40	0.0
41	0.0014456298609304074
42	0.005059704513256426
43	0.002891259721860815
44	0.0036140746523260184
45	0.0021684447913956108
46	0.002529852256628213
47	0.0021684447913956108
48	0.0032526671870934166
49	0.004698297048023823
50	0.0018070373261630092
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	276696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.623095382658224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.314109273602675	15.55570011854165
2	14.033389156374293	7.752912944169775
3	7.672179191961483	6.3578801283719315
4	4.873613146326146	5.384971231965768
5	3.316673644546787	4.580839621823228
6	2.4073686414067406	3.989938416167924
7	1.7806677831274857	3.443128921270998
8	1.4169457818714675	3.1312342787752625
9	1.0715407159305004	2.663934426229508
>10	6.5339124973832945	32.4926995692023
>50	0.44091480008373457	8.174675456096221
>100	0.13737701486288464	6.274395003903201
>500	0.0013083525225036634	0.19768988348223324
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	547	0.19768988348223324	No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA	471	0.17022291612455548	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	447	0.16154913695897302	No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT	432	0.156128024980484	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	416	0.15034550553676235	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	400	0.14456298609304075	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	393	0.14203313383641253	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	347	0.12540839043571284	No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC	319	0.11528898140919998	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.10372394252175672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	3.6140746523260185E-4	0.0
26	0.0	0.0	0.0	7.228149304652037E-4	0.0
27	0.0	0.0	0.0	7.228149304652037E-4	0.0
28	0.0	0.0	0.0	0.0010842223956978056	0.0
29	0.0	0.0	0.0	0.0014456298609304074	0.0
30	0.0	0.0	0.0	0.004698297048023824	0.0
31	0.0	0.0	0.0	0.012287853817908463	0.0
32	0.0	0.0	0.0	0.01770896579639749	0.0
33	0.0	0.0	0.0	0.02313007777488652	0.0
34	0.0	0.0	0.0	0.03288807933616677	0.0
35	0.0	0.0	0.0	0.04481452568884263	0.0
36	0.0	0.0	0.0	0.0592708242981467	0.0
37	0.0	0.0	0.0	0.08167808714256802	0.0
38	0.0	0.0	0.0	0.11275912915257177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTTAT	20	7.8422966E-4	44.004337	1
CTGGGCT	25	0.0023485315	35.203472	37
CGCCAGC	25	0.0023485315	35.203472	42
GGGCCCG	30	0.0057346923	29.336224	34
AGGGCCC	30	0.0057346923	29.336224	33
AGTAGTG	30	0.005739762	29.330923	7
GTAAAGG	30	0.005739762	29.330923	3
GGGGTCC	100	6.002665E-11	26.397831	6
AATAACC	50	8.323276E-5	26.397831	2
AGATAAT	105	1.10958354E-10	25.140793	18
TTCGTTT	45	0.0013946911	24.446856	28
TTAGAAT	110	1.9826984E-10	24.002367	42
CGTTTAT	110	1.9826984E-10	24.002367	36
AGGGGTC	110	2.0008883E-10	23.99803	5
TCGGTTT	55	1.5901768E-4	23.99803	13
GTCGGTT	55	1.5901768E-4	23.99803	12
GCGTTTA	115	3.4742698E-10	22.958784	35
TAGGGCG	115	3.4742698E-10	22.958784	31
GAAGATA	125	4.0017767E-11	22.87812	16
TTTAATC	145	0.0	22.760866	40
>>END_MODULE