FastQCFastQC Report
Thu 2 Feb 2017
SRR4062119_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062119_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003522
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14690.14638443402336968No Hit
GCGCAAGACGGACCAGAGCGAAAGC10670.10632552151323041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4550.015.2394371
ACGAACG456.7595E-414.77773915
GGTCTAT400.005282707514.2478291
CGTTATT759.663127E-713.9332972
GCGTTAT759.679461E-713.9312111
CGAACGA500.001498929313.29996516
GTATTAG655.4565113E-513.1518431
CGGTTCT1850.012.83844512
TTGCGCG905.4085285E-712.66473818
ACGGTAT1457.2759576E-1212.4488659
ATTTGCG853.9394836E-612.294085516
TAACGAA550.00306605212.0914813
TAGGGGT550.00306605212.091484
ATAACGA550.00306605212.0914812
GTCCTAG1103.815512E-812.0890681
TTTATAC951.0379445E-612.0005673
CCGACCA1353.6925485E-1011.9635289
CGCGTAA802.8625958E-511.87556210
TCGCGTA802.8625958E-511.8755629
GCGCCGA1201.0015356E-811.87319219
Produced by FastQC (version 0.11.5)