##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062119_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003522
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319116073190223	32.0	32.0	32.0	32.0	32.0
2	31.036127758036198	32.0	32.0	32.0	32.0	32.0
3	31.043901379341957	32.0	32.0	32.0	32.0	32.0
4	31.03720396762602	32.0	32.0	32.0	32.0	32.0
5	31.029133392192698	32.0	32.0	32.0	32.0	32.0
6	34.560532803466195	36.0	36.0	36.0	32.0	36.0
7	34.539716119825975	36.0	36.0	36.0	32.0	36.0
8	34.522325370046694	36.0	36.0	36.0	32.0	36.0
9	34.61395564820702	36.0	36.0	36.0	32.0	36.0
10	34.4486359043449	36.0	36.0	36.0	32.0	36.0
11	34.544141533518946	36.0	36.0	36.0	32.0	36.0
12	34.482630176518306	36.0	36.0	36.0	32.0	36.0
13	34.51733096035762	36.0	36.0	36.0	32.0	36.0
14	34.47258953964138	36.0	36.0	36.0	32.0	36.0
15	34.437269935287915	36.0	36.0	36.0	32.0	36.0
16	34.435523087685176	36.0	36.0	36.0	32.0	36.0
17	34.38511462628622	36.0	36.0	36.0	32.0	36.0
18	34.38073505114985	36.0	36.0	36.0	32.0	36.0
19	34.37586420626553	36.0	36.0	36.0	32.0	36.0
20	34.34536063982653	36.0	36.0	36.0	32.0	36.0
21	34.32176873053107	36.0	36.0	36.0	32.0	36.0
22	34.3008514013644	36.0	36.0	36.0	32.0	36.0
23	34.28461060146165	36.0	36.0	36.0	32.0	36.0
24	34.25580505459771	36.0	36.0	36.0	32.0	36.0
25	33.85460807037614	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	18.0
5	71.0
6	212.0
7	36.0
8	162.0
9	140.0
10	70.0
11	20.0
12	39.0
13	43.0
14	171.0
15	279.0
16	463.0
17	562.0
18	788.0
19	1030.0
20	1336.0
21	1757.0
22	2306.0
23	2927.0
24	4101.0
25	5715.0
26	8203.0
27	10810.0
28	14809.0
29	20809.0
30	28816.0
31	41512.0
32	63609.0
33	95905.0
34	229322.0
35	467480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.510623201937996	17.78286436322073	11.945559141852954	25.76095329298832
2	16.546456473214285	19.46109693877551	37.661830357142854	26.33061623086735
3	19.084418269781718	22.796171457327493	28.378748698325385	29.7406615745654
4	12.75343426853867	15.68302678051775	35.28686008594641	36.276678864997166
5	14.891445204250484	36.30893757850037	33.55938116788612	15.240236049363027
6	34.749739259796954	34.96580963073603	16.46462209122617	13.819829018240846
7	30.658113144307276	30.213611951256492	20.152383308461484	18.97589159597475
8	27.929653404358255	33.47707900026435	19.0696932062466	19.523574389130793
9	26.853860957061237	14.560531374796609	18.900797201862364	39.684810466279785
10	16.484305934988715	26.79984354826157	31.139583287601795	25.576267229147913
11	37.00214538741705	21.386419198722745	21.977049343910593	19.63438606994961
12	24.282353222900042	23.846227527703114	28.657080083094367	23.21433916630248
13	29.324380278668816	19.70347163674278	25.016038672407	25.956109412181412
14	23.314455137765602	19.88854648535352	25.075444611932866	31.721553764948005
15	24.963256460427232	27.517493716430675	21.964604471885828	25.554645351256266
16	25.535402348617335	25.982439740736268	23.848711612497095	24.633446298149302
17	23.765787139623214	26.399080532245723	25.20514925886868	24.62998306926238
18	24.41631951094937	25.393343388878247	26.124465300389453	24.06587179978293
19	25.37779537658851	25.31306322705225	25.156768499281362	24.15237289707788
20	25.544023570198938	24.773543768239676	24.874578854244174	24.807853807317215
21	26.149275107915965	24.607732448196348	24.555669477934078	24.68732296595361
22	25.625019949567495	24.735668230712758	24.918905008139426	24.720406811580325
23	24.284020316799257	24.859057939578165	25.38472968943381	25.47219205418877
24	24.51381699291646	25.16990722178004	25.486681707470332	24.829594077833168
25	24.623570655615897	24.93275286866239	25.353140942207858	25.090535533513858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	385.0
1	385.0
2	348.5
3	312.0
4	312.0
5	312.0
6	707.0
7	1102.0
8	1102.0
9	1102.0
10	1316.5
11	1531.0
12	1531.0
13	1531.0
14	2185.5
15	2840.0
16	2840.0
17	2840.0
18	4824.0
19	6808.0
20	6808.0
21	6808.0
22	10693.0
23	14578.0
24	14578.0
25	14578.0
26	21356.5
27	28135.0
28	28135.0
29	28135.0
30	35739.5
31	43344.0
32	43344.0
33	43344.0
34	53033.5
35	62723.0
36	62723.0
37	62723.0
38	73171.5
39	83620.0
40	83620.0
41	83620.0
42	95043.0
43	106466.0
44	106466.0
45	106466.0
46	118291.5
47	130117.0
48	130117.0
49	130117.0
50	132612.0
51	135107.0
52	135107.0
53	135107.0
54	126869.5
55	118632.0
56	118632.0
57	118632.0
58	108765.5
59	98899.0
60	98899.0
61	98899.0
62	86686.0
63	74473.0
64	74473.0
65	74473.0
66	60709.5
67	46946.0
68	46946.0
69	46946.0
70	35424.0
71	23902.0
72	23902.0
73	23902.0
74	17868.0
75	11834.0
76	11834.0
77	11834.0
78	9486.5
79	7139.0
80	7139.0
81	7139.0
82	4870.5
83	2602.0
84	2602.0
85	2602.0
86	1866.0
87	1130.0
88	1130.0
89	1130.0
90	779.0
91	428.0
92	428.0
93	428.0
94	260.5
95	93.0
96	93.0
97	93.0
98	234.5
99	376.0
100	376.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010961393970436125
2	1.992980721897477E-4
3	6.97543252664117E-4
4	0.010562797826056628
5	0.034079970344446854
6	0.06058661394568331
7	0.10463148789961756
8	0.10532903115228166
9	0.11369955018425107
10	0.1291451507789565
11	0.13671847752216693
12	0.12834795849019753
13	0.12525883837125643
14	0.11270305982330232
15	0.13004199210381037
16	0.11409814632863056
17	0.11987779042213324
18	0.1086174493434125
19	0.0925739545321378
20	0.08968413248538647
21	0.08868764212443772
22	0.09905114187830462
23	0.08021747405637344
24	0.09127851706290445
25	0.08739220465520438
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1003522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.1023045347507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90050177088578	45.261986773018926
2	13.388438598399699	15.557982733780602
3	4.105973963952339	7.1569964899594884
4	1.6122360631270982	3.746985228868728
5	0.8369650538026417	2.431479922049255
6	0.4777971455572721	1.6656669154201935
7	0.3192751934270412	1.2985437163222568
8	0.220651609179237	1.0256293594091856
9	0.16280880602572168	0.8513610145781072
>10	0.8347152433217455	8.73312032543768
>50	0.0735035991971673	2.9955482997300704
>100	0.06215378312063032	7.158410684373269
>500	0.004635778968929776	1.863299580232928
>1k	3.4339103473553895E-4	0.25298895681929034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1469	0.14638443402336968	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1067	0.10632552151323041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.964903609487386E-5
2	0.0	0.0	0.0	0.0	9.964903609487386E-5
3	0.0	0.0	0.0	0.0	9.964903609487386E-5
4	0.0	0.0	0.0	0.0	9.964903609487386E-5
5	0.0	0.0	0.0	0.0	9.964903609487386E-5
6	0.0	0.0	0.0	0.0	9.964903609487386E-5
7	0.0	0.0	0.0	0.0	9.964903609487386E-5
8	0.0	0.0	0.0	0.0	9.964903609487386E-5
9	0.0	0.0	0.0	0.0	9.964903609487386E-5
10	0.0	0.0	0.0	0.0	9.964903609487386E-5
11	0.0	0.0	0.0	0.0	9.964903609487386E-5
12	0.0	0.0	0.0	0.0	4.982451804743693E-4
13	0.0	0.0	0.0	0.0	5.978942165692431E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	455	0.0	15.239437	1
ACGAACG	45	6.7595E-4	14.777739	15
GGTCTAT	40	0.0052827075	14.247829	1
CGTTATT	75	9.663127E-7	13.933297	2
GCGTTAT	75	9.679461E-7	13.931211	1
CGAACGA	50	0.0014989293	13.299965	16
GTATTAG	65	5.4565113E-5	13.151843	1
CGGTTCT	185	0.0	12.838445	12
TTGCGCG	90	5.4085285E-7	12.664738	18
ACGGTAT	145	7.2759576E-12	12.448865	9
ATTTGCG	85	3.9394836E-6	12.2940855	16
TAACGAA	55	0.003066052	12.09148	13
TAGGGGT	55	0.003066052	12.09148	4
ATAACGA	55	0.003066052	12.09148	12
GTCCTAG	110	3.815512E-8	12.089068	1
TTTATAC	95	1.0379445E-6	12.000567	3
CCGACCA	135	3.6925485E-10	11.963528	9
CGCGTAA	80	2.8625958E-5	11.875562	10
TCGCGTA	80	2.8625958E-5	11.875562	9
GCGCCGA	120	1.0015356E-8	11.873192	19
>>END_MODULE