FastQCFastQC Report
Thu 2 Feb 2017
SRR4062119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003522
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11030.10991288681264585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG300.005745636329.3322055
GGTATCA5000.025.0927891
GTATCAA10850.024.1413351
GTATATG1452.928573E-1019.7341941
GTCCTAC2950.019.3997171
TCTATAC1252.287743E-819.3592553
ACACGCT2600.018.6146689
AACGTAT600.007410763818.33354223
TAGGGCG600.00741289418.3326285
ATAGCAC851.4309474E-418.1169513
ATACCGT2800.018.0707346
CAATACG2450.017.95938738
ACCGTCG2700.017.9252368
CCGTCGT2850.017.7537029
CGTCGTA2800.017.2850510
TAGGACC5750.017.2167284
CGCAATA2450.017.06056636
GTGTAGG2850.016.9911161
AATACGA2600.016.9232739
CTACTAT1053.756857E-516.770451
Produced by FastQC (version 0.11.5)