##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003522
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219169086477425	32.0	32.0	32.0	32.0	32.0
2	31.30446866137464	32.0	32.0	32.0	32.0	32.0
3	31.4783203557072	32.0	32.0	32.0	32.0	32.0
4	31.57901471019071	32.0	32.0	32.0	32.0	32.0
5	31.52861222773392	32.0	32.0	32.0	32.0	32.0
6	35.14298141944073	36.0	36.0	36.0	36.0	36.0
7	35.150699237286275	36.0	36.0	36.0	36.0	36.0
8	35.09608857603521	36.0	36.0	36.0	36.0	36.0
9	35.207630724588	36.0	36.0	36.0	36.0	36.0
10	35.05386827593217	36.0	36.0	36.0	36.0	36.0
11	35.213927547178834	36.0	36.0	36.0	36.0	36.0
12	35.11144150302634	36.0	36.0	36.0	36.0	36.0
13	35.16548017881023	36.0	36.0	36.0	36.0	36.0
14	35.12734050673528	36.0	36.0	36.0	36.0	36.0
15	35.099812460514066	36.0	36.0	36.0	36.0	36.0
16	35.10386319383132	36.0	36.0	36.0	36.0	36.0
17	35.08354575186194	36.0	36.0	36.0	36.0	36.0
18	35.08162152897495	36.0	36.0	36.0	36.0	36.0
19	35.07758375003239	36.0	36.0	36.0	36.0	36.0
20	35.079109376775	36.0	36.0	36.0	36.0	36.0
21	35.066354300154856	36.0	36.0	36.0	36.0	36.0
22	35.04850317182882	36.0	36.0	36.0	36.0	36.0
23	35.01704895358547	36.0	36.0	36.0	36.0	36.0
24	34.994162559465565	36.0	36.0	36.0	36.0	36.0
25	34.98163169317663	36.0	36.0	36.0	36.0	36.0
26	34.92803645560336	36.0	36.0	36.0	32.0	36.0
27	34.917165742255776	36.0	36.0	36.0	32.0	36.0
28	34.89167153286126	36.0	36.0	36.0	32.0	36.0
29	34.86602386395116	36.0	36.0	36.0	32.0	36.0
30	34.85515713656502	36.0	36.0	36.0	32.0	36.0
31	34.8509011262334	36.0	36.0	36.0	32.0	36.0
32	34.823274427466465	36.0	36.0	36.0	32.0	36.0
33	34.79866111555103	36.0	36.0	36.0	32.0	36.0
34	34.79355609543189	36.0	36.0	36.0	32.0	36.0
35	34.78341182355743	36.0	36.0	36.0	32.0	36.0
36	34.75232531025728	36.0	36.0	36.0	32.0	36.0
37	34.732957523601875	36.0	36.0	36.0	32.0	36.0
38	34.712638088651765	36.0	36.0	36.0	32.0	36.0
39	34.69183635236696	36.0	36.0	36.0	32.0	36.0
40	34.6868977461381	36.0	36.0	36.0	32.0	36.0
41	34.65194086427602	36.0	36.0	36.0	32.0	36.0
42	34.61077086501342	36.0	36.0	36.0	32.0	36.0
43	34.6243390777681	36.0	36.0	36.0	32.0	36.0
44	34.56959090084722	36.0	36.0	36.0	32.0	36.0
45	34.560405252699994	36.0	36.0	36.0	32.0	36.0
46	34.529689433814106	36.0	36.0	36.0	32.0	36.0
47	34.498001040335936	36.0	36.0	36.0	32.0	36.0
48	34.47794069287968	36.0	36.0	36.0	32.0	36.0
49	34.45845033790988	36.0	36.0	36.0	32.0	36.0
50	34.02228650692262	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	8.0
21	18.0
22	57.0
23	179.0
24	506.0
25	1162.0
26	2408.0
27	4944.0
28	8461.0
29	13952.0
30	20968.0
31	31931.0
32	48885.0
33	83944.0
34	188302.0
35	597795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30297871322459	18.159744510850715	12.454198945030761	26.08307783089393
2	15.93075655173926	20.01985103749574	37.179338976396	26.870053434369
3	18.373226243622447	23.394252232142858	28.63869180484694	29.593829719387756
4	12.524189803512032	16.157692606639415	35.38228359717077	35.93583399267779
5	14.670032146779043	36.661876869665036	33.178744462004815	15.489346521551099
6	34.91333523330829	35.221350403877544	16.352107876060515	13.513206486753655
7	30.635800709899737	30.20352319132017	20.116948108760944	19.043727990019153
8	27.999386161937657	33.67150894549397	18.88070216696794	19.448402725600435
9	27.057204525660623	14.359027505126942	18.65170868202192	39.93205928719051
10	16.521726999235693	26.906960591756423	30.921326011114864	25.64998639789302
11	37.231171812875054	21.195250328343572	21.995731035293694	19.577846823487675
12	24.293637807641485	23.80446068945175	28.650991208962033	23.250910293944727
13	29.45944383879975	19.54217246856571	25.041204876425233	25.957178816209307
14	23.425096809038568	19.64112396140792	25.065917837376762	31.867861392176756
15	25.08684413495668	27.3433965573251	21.94241880098294	25.62734050673528
16	25.585865682127324	25.962143067119076	23.692620439156364	24.759370811597236
17	23.943172147695815	26.33783813409173	25.093420971338944	24.625568746873512
18	24.65690768494898	25.351064253826532	26.142677774234695	23.849350286989797
19	25.659925741538302	25.10617604795909	25.16187985913612	24.072018351366488
20	25.725620091657277	24.60964942437677	24.95353859062242	24.711191893343535
21	26.385071777200697	24.523627782948456	24.588300007374027	24.503000432476817
22	25.796145973879998	24.61430840579479	24.985002820067724	24.60454280025749
23	24.470240283960177	24.567597489240384	25.450189881427494	25.511972345371948
24	24.797087830451623	24.97077765808714	25.437637579184326	24.79449693227691
25	24.886200573979593	24.625418526785715	25.413643973214285	25.074736926020407
26	24.49978326183465	25.56852662889942	25.61566095175458	24.31602915751135
27	25.024339349827958	24.929772131867956	25.121397801137796	24.924490717166293
28	24.70495970630997	24.8348043411755	25.536443691972256	24.923792260542278
29	24.68799700253907	24.98246166478661	25.33601855874744	24.993522773926873
30	24.643525867730133	25.029471584211006	25.61910256908508	24.707899978973778
31	25.067612140489757	24.844796007645105	24.975835012426284	25.111756839438854
32	24.864775449519488	25.06028802729603	24.797711247254654	25.277225275929833
33	24.547293526785715	24.77927694515306	25.444036989795922	25.229392538265305
34	25.079047464352655	24.8646003880376	25.620645912202257	24.435706235407483
35	25.421914859693878	24.710020727040817	25.51369180484694	24.354372608418366
36	24.390321677373965	25.23434985416349	25.325329514778467	25.049998953684078
37	25.326980893852408	24.978400576383237	25.046960684564706	24.647657845199646
38	24.803741223411148	24.846789607004133	25.39206913251528	24.95740003706944
39	25.38679081706882	24.760292332742743	24.849379281205845	25.003537568982598
40	25.612916919526345	24.78742348192016	25.23016459047693	24.369495008076562
41	24.536303964173857	25.17386421501266	25.738281417380744	24.55155040343274
42	25.6081652746622	25.340294518315964	25.025287278431126	24.02625292859072
43	24.96088588244439	24.69062476955058	25.363287937704413	24.98520141030062
44	24.603131894742507	25.258450076832467	25.099304222696574	25.03911380572845
45	24.702190546231467	25.30408751821137	25.13099231285887	24.862729622698293
46	24.698703933640125	25.037618410799023	25.061235797181457	25.202441858379387
47	24.963478337123348	24.858547065611443	25.3963572007964	24.781617396468807
48	25.42815914984026	25.42227962235496	24.456641972651227	24.692919255153555
49	24.794142823972805	25.487724305347253	24.754780081854573	24.963352788825365
50	25.10296390558312	25.543319578239114	24.59813434285959	24.75558217331817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	78.0
2	25.0
3	82.5
4	140.0
5	140.0
6	140.0
7	108.0
8	76.0
9	113.0
10	150.0
11	288.5
12	427.0
13	769.5
14	1112.0
15	1458.5
16	1805.0
17	1840.0
18	1875.0
19	1936.0
20	1997.0
21	2326.0
22	2655.0
23	3357.5
24	4060.0
25	5180.5
26	6301.0
27	8135.5
28	9970.0
29	11575.5
30	13181.0
31	14871.0
32	16561.0
33	19503.5
34	22446.0
35	25816.0
36	29186.0
37	34059.5
38	38933.0
39	41883.0
40	44833.0
41	48380.0
42	51927.0
43	53720.5
44	55514.0
45	59964.5
46	64415.0
47	68224.0
48	72033.0
49	74788.0
50	77543.0
51	75992.5
52	74442.0
53	73995.5
54	73549.0
55	73718.5
56	73888.0
57	71100.5
58	68313.0
59	62400.0
60	56487.0
61	49704.0
62	42921.0
63	36918.5
64	30916.0
65	26170.0
66	21424.0
67	18121.0
68	14818.0
69	13040.0
70	11262.0
71	8781.0
72	6300.0
73	5499.5
74	4699.0
75	3496.0
76	2293.0
77	2074.0
78	1855.0
79	1545.0
80	1235.0
81	980.5
82	726.0
83	603.0
84	480.0
85	381.0
86	282.0
87	192.0
88	102.0
89	67.5
90	33.0
91	27.0
92	21.0
93	15.5
94	10.0
95	13.0
96	16.0
97	13.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026905239745615938
2	0.0047831537325539455
3	1.992980721897477E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	9.964903609487386E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.97543252664117E-4
17	0.0
18	1.992980721897477E-4
19	0.0
20	9.964903609487386E-5
21	0.0
22	0.0
23	9.964903609487386E-5
24	8.968413248538646E-4
25	1.992980721897477E-4
26	6.97543252664117E-4
27	2.989471082846216E-4
28	0.0014947355414231078
29	5.978942165692432E-4
30	0.00129543746923336
31	3.985961443794954E-4
32	5.978942165692432E-4
33	1.992980721897477E-4
34	0.0010961393970436125
35	1.992980721897477E-4
36	9.964903609487386E-5
37	0.0022919278301820984
38	0.0
39	7.971922887589908E-4
40	9.964903609487386E-5
41	8.968413248538646E-4
42	0.005281398913028314
43	0.0047831537325539455
44	0.0039859614437949544
45	0.002790173010656468
46	0.0023915768662769728
47	7.971922887589908E-4
48	0.0039859614437949544
49	0.003487716263320585
50	0.0014947355414231078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1003522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.37707362641099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24345644038053	47.24110930285737
2	13.500018231822775	16.301831894813088
3	3.8701569209186304	7.010062480802046
4	1.5933092796108088	3.847974067388226
5	0.7980722414528465	2.409263324069668
6	0.4795213526594878	1.737125760897485
7	0.308227564471855	1.302691484766457
8	0.21829863216568912	1.0544186069450097
9	0.14146204529249853	0.7686957891571083
>10	0.7302768822497234	7.890124170219942
>50	0.061841085392394114	2.631019676476652
>100	0.05121566227034948	6.068718851293875
>500	0.003977914859826634	1.626584228319948
>1k	1.6574645249277644E-4	0.11038036199316492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1103	0.10991288681264585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.964903609487386E-5
2	0.0	0.0	0.0	0.0	9.964903609487386E-5
3	0.0	0.0	0.0	0.0	9.964903609487386E-5
4	0.0	0.0	0.0	0.0	9.964903609487386E-5
5	0.0	0.0	0.0	0.0	9.964903609487386E-5
6	0.0	0.0	0.0	0.0	9.964903609487386E-5
7	0.0	0.0	0.0	0.0	9.964903609487386E-5
8	0.0	0.0	0.0	0.0	9.964903609487386E-5
9	0.0	0.0	0.0	0.0	9.964903609487386E-5
10	0.0	0.0	0.0	0.0	2.9894710828462154E-4
11	0.0	0.0	0.0	0.0	2.9894710828462154E-4
12	0.0	0.0	0.0	0.0	7.971922887589908E-4
13	0.0	0.0	0.0	0.0	9.964903609487386E-4
14	0.0	0.0	0.0	0.0	9.964903609487386E-4
15	0.0	0.0	0.0	0.0	0.0011957884331384862
16	0.0	0.0	0.0	0.0	0.0011957884331384862
17	0.0	0.0	0.0	0.0	0.0011957884331384862
18	0.0	0.0	0.0	0.0	0.0011957884331384862
19	0.0	0.0	0.0	0.0	0.0011957884331384862
20	0.0	0.0	0.0	0.0	0.00129543746923336
21	0.0	0.0	0.0	0.0	0.0014947355414231078
22	0.0	0.0	0.0	9.964903609487386E-5	0.0014947355414231078
23	0.0	0.0	0.0	1.992980721897477E-4	0.0014947355414231078
24	0.0	0.0	0.0	1.992980721897477E-4	0.0014947355414231078
25	0.0	0.0	0.0	3.985961443794954E-4	0.0014947355414231078
26	0.0	0.0	0.0	4.982451804743693E-4	0.0015943845775179817
27	0.0	0.0	0.0	6.97543252664117E-4	0.0015943845775179817
28	0.0	0.0	0.0	0.00129543746923336	0.0015943845775179817
29	0.0	0.0	0.0	0.0018933316858026031	0.0015943845775179817
30	0.0	0.0	0.0	0.0029894710828462156	0.0015943845775179817
31	0.0	0.0	0.0	0.006078591201787305	0.0016940336136128556
32	0.0	0.0	0.0	0.010164201681677133	0.0016940336136128556
33	0.0	0.0	0.0	0.013054023728428475	0.0016940336136128556
34	0.0	0.0	0.0	0.016442090955654187	0.0017936826497077295
35	0.0	0.0	0.0	0.0217234898686825	0.0017936826497077295
36	0.0	0.0	0.0	0.028101028178754428	0.0017936826497077295
37	0.0	0.0	0.0	0.03806593178824181	0.0017936826497077295
38	0.0	0.0	0.0	0.05141890262495491	0.0018933316858026031
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGCCG	30	0.0057456363	29.332205	5
GGTATCA	500	0.0	25.092789	1
GTATCAA	1085	0.0	24.141335	1
GTATATG	145	2.928573E-10	19.734194	1
GTCCTAC	295	0.0	19.399717	1
TCTATAC	125	2.287743E-8	19.359255	3
ACACGCT	260	0.0	18.614668	9
AACGTAT	60	0.0074107638	18.333542	23
TAGGGCG	60	0.007412894	18.332628	5
ATAGCAC	85	1.4309474E-4	18.116951	3
ATACCGT	280	0.0	18.070734	6
CAATACG	245	0.0	17.959387	38
ACCGTCG	270	0.0	17.925236	8
CCGTCGT	285	0.0	17.753702	9
CGTCGTA	280	0.0	17.28505	10
TAGGACC	575	0.0	17.216728	4
CGCAATA	245	0.0	17.060566	36
GTGTAGG	285	0.0	16.991116	1
AATACGA	260	0.0	16.92327	39
CTACTAT	105	3.756857E-5	16.77045	1
>>END_MODULE