FastQCFastQC Report
Thu 2 Feb 2017
SRR4062118_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062118_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679486
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14120.20780413430151615No Hit
TATCAACGCAGAGTACTTTTTTTTT9650.14201911444827414No Hit
GTTCTAAGTTGGTTGTTAAGCGTGC8200.12067945476433657No Hit
CCTTTGTGCTGTCCGGTGCGCTCTC8130.11964926429683614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACG250.006026083619.001416
GTTACGG453.531909E-516.89013514
GCCGCGC402.7551377E-416.627458
CGGATCT551.1272265E-515.54430718
TAGCACC456.762938E-414.7755994
CGCGCCA400.00526730814.25315310
GAGCGGT602.5604888E-514.2521027
TAGACTG759.649575E-713.9333345
GCGGTCG551.955142E-413.8202199
TACGGAT551.9563656E-413.819200516
TCGACTC500.00149719613.30098113
AATCGCT500.00149719613.30098115
TAATTCC500.001499694113.29803854
AAGACGG1102.733941E-912.9545465
TCAGGAC1102.739398E-912.9526353
GGTATCA3850.012.822661
TGCGCTA751.4723219E-512.6694710
GCGCTAT751.4723219E-512.6694711
CGAGCGG604.0816626E-412.6685346
GCTATCG751.47455685E-512.66760113
Produced by FastQC (version 0.11.5)