##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062118_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679486
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152541774223458	32.0	32.0	32.0	32.0	32.0
2	30.622919383180815	32.0	32.0	32.0	32.0	32.0
3	30.673475244523065	32.0	32.0	32.0	32.0	32.0
4	30.67477917131479	32.0	32.0	32.0	32.0	32.0
5	30.57583673541471	32.0	32.0	32.0	32.0	32.0
6	34.21761596265412	36.0	36.0	36.0	32.0	36.0
7	34.14155258533656	36.0	36.0	36.0	32.0	36.0
8	34.08749407640481	36.0	36.0	36.0	32.0	36.0
9	34.22946315303038	36.0	36.0	36.0	32.0	36.0
10	33.88966807263137	36.0	36.0	36.0	32.0	36.0
11	34.25440406424856	36.0	36.0	36.0	32.0	36.0
12	34.0351736459618	36.0	36.0	36.0	32.0	36.0
13	34.14425757116408	36.0	36.0	36.0	32.0	36.0
14	34.039994937349704	36.0	36.0	36.0	32.0	36.0
15	33.988710584176864	36.0	36.0	36.0	32.0	36.0
16	33.992239722378386	36.0	36.0	36.0	32.0	36.0
17	33.91880333075295	36.0	36.0	36.0	32.0	36.0
18	33.92495356784393	36.0	36.0	36.0	32.0	36.0
19	33.92234277085915	36.0	36.0	36.0	32.0	36.0
20	33.90095601675384	36.0	36.0	36.0	32.0	36.0
21	33.90039824220072	36.0	36.0	36.0	32.0	36.0
22	33.8654041437204	36.0	36.0	36.0	32.0	36.0
23	33.838229190888406	36.0	36.0	36.0	32.0	36.0
24	33.80262286493026	36.0	36.0	36.0	32.0	36.0
25	33.45135734952596	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	20.0
5	61.0
6	133.0
7	29.0
8	102.0
9	103.0
10	53.0
11	20.0
12	29.0
13	33.0
14	90.0
15	158.0
16	252.0
17	349.0
18	495.0
19	703.0
20	1030.0
21	1541.0
22	2278.0
23	3358.0
24	4575.0
25	6570.0
26	9092.0
27	11576.0
28	15292.0
29	20093.0
30	26177.0
31	35284.0
32	48913.0
33	67917.0
34	142311.0
35	280848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75149342363782	17.839697768437286	12.076458580765557	25.33235022715934
2	16.54772819923383	19.42153078149122	37.94296546050453	26.08777555877042
3	18.912845116854065	23.11326308353447	28.906075234002472	29.067816565608993
4	12.706026770601206	15.758711337323117	35.566277402774794	35.96898448930089
5	14.714447238400338	36.5211912066945	33.7155720183027	15.048789536602458
6	33.77904947596446	35.68654766831062	16.7984425460602	13.735960309664726
7	29.88020749693926	31.065314980177	20.351024004172334	18.70345351871141
8	28.415066262974502	33.039109519915726	19.048524092613466	19.497300124496306
9	27.05872298540803	14.41220285238138	19.070198523268616	39.458875638941976
10	16.562823282531618	26.830939630348986	31.226992599316816	25.379244487802577
11	36.66904436970728	21.825397995065778	21.643680566404235	19.86187706882271
12	23.8203306905597	24.17091573580123	29.068643343452504	22.940110230186566
13	29.18689830076378	20.38641377308248	24.96702765550789	25.459660270645845
14	23.112194230718227	19.881596822150644	25.477900332695842	31.528308614435286
15	25.359601178310022	27.260723932690734	22.423699416002677	24.95597547299657
16	25.57292119451104	26.170854508296358	23.69645969844004	24.55976459875256
17	23.134683533112803	26.336115756669344	25.634002048138626	24.895198662079228
18	24.093318069104523	25.91294380082244	25.707259790986146	24.286478339086887
19	25.48687428184202	25.470817005980965	25.26884888482956	23.773459827347455
20	25.892879502329695	24.92107895345463	24.95908479574806	24.22695674846761
21	26.16371564092744	24.311345490970158	25.0791032024276	24.44583566567481
22	25.453273127720855	24.718725369594203	25.43677305774288	24.391228444942065
23	24.4377049228621	24.82965441867453	25.564504488050034	25.168136170413334
24	24.75270423352675	25.321024154333184	25.440197512532354	24.486074099607716
25	24.614401093567484	25.129514979841765	25.31614617627	24.939937750320748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	507.0
1	507.0
2	391.5
3	276.0
4	276.0
5	276.0
6	578.0
7	880.0
8	880.0
9	880.0
10	1088.0
11	1296.0
12	1296.0
13	1296.0
14	1677.0
15	2058.0
16	2058.0
17	2058.0
18	3523.0
19	4988.0
20	4988.0
21	4988.0
22	7494.5
23	10001.0
24	10001.0
25	10001.0
26	15171.5
27	20342.0
28	20342.0
29	20342.0
30	25488.5
31	30635.0
32	30635.0
33	30635.0
34	36855.5
35	43076.0
36	43076.0
37	43076.0
38	50385.5
39	57695.0
40	57695.0
41	57695.0
42	65838.5
43	73982.0
44	73982.0
45	73982.0
46	81290.5
47	88599.0
48	88599.0
49	88599.0
50	90384.5
51	92170.0
52	92170.0
53	92170.0
54	85886.0
55	79602.0
56	79602.0
57	79602.0
58	70904.0
59	62206.0
60	62206.0
61	62206.0
62	54711.5
63	47217.0
64	47217.0
65	47217.0
66	38894.0
67	30571.0
68	30571.0
69	30571.0
70	23508.5
71	16446.0
72	16446.0
73	16446.0
74	12672.5
75	8899.0
76	8899.0
77	8899.0
78	6816.0
79	4733.0
80	4733.0
81	4733.0
82	3302.5
83	1872.0
84	1872.0
85	1872.0
86	1350.0
87	828.0
88	828.0
89	828.0
90	544.0
91	260.0
92	260.0
93	260.0
94	168.0
95	76.0
96	76.0
97	76.0
98	173.5
99	271.0
100	271.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010301904675004343
2	4.4151020035732887E-4
3	8.830204007146577E-4
4	0.012362285610005208
5	0.035615156162157866
6	0.06313595865109804
7	0.10728697868683092
8	0.110524720156118
9	0.12141530509826544
10	0.13245306010719868
11	0.14128326411434525
12	0.13333608050791335
13	0.1305398492389836
14	0.11597001262719173
15	0.1305398492389836
16	0.11994360443040768
17	0.12082662483112236
18	0.11405680175897664
19	0.09801526447932701
20	0.09463035294325417
21	0.09212846180789597
22	0.10316621681682918
23	0.08418127820146405
24	0.09463035294325417
25	0.08933223053896622
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	679486.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.73139991472088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5527643892499	48.313507484451016
2	13.265565163080081	16.11272686027612
3	3.6138680277903195	6.584257933042725
4	1.314992952572884	3.194454515509736
5	0.6203124064125887	1.8836220412952898
6	0.30826453047947405	1.1232801888043562
7	0.21803513519230358	0.9269105293576816
8	0.16038326878645423	0.7792240349040258
9	0.10752487929185056	0.587712280054992
>10	0.6805679482305667	7.960642591837713
>50	0.09030042530436455	3.806918685040462
>100	0.06547370036421742	7.800218400975957
>500	0.001703776589374431	0.7178051612649728
>1k	2.4339665562491872E-4	0.208719293184955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1412	0.20780413430151615	No Hit
TATCAACGCAGAGTACTTTTTTTTT	965	0.14201911444827414	No Hit
GTTCTAAGTTGGTTGTTAAGCGTGC	820	0.12067945476433657	No Hit
CCTTTGTGCTGTCCGGTGCGCTCTC	813	0.11964926429683614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.471700667857763E-4
11	0.0	0.0	0.0	0.0	1.471700667857763E-4
12	0.0	0.0	0.0	0.0	1.471700667857763E-4
13	0.0	0.0	0.0	0.0	1.471700667857763E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGACG	25	0.0060260836	19.0014	16
GTTACGG	45	3.531909E-5	16.890135	14
GCCGCGC	40	2.7551377E-4	16.62745	8
CGGATCT	55	1.1272265E-5	15.544307	18
TAGCACC	45	6.762938E-4	14.775599	4
CGCGCCA	40	0.005267308	14.253153	10
GAGCGGT	60	2.5604888E-5	14.252102	7
TAGACTG	75	9.649575E-7	13.933334	5
GCGGTCG	55	1.955142E-4	13.820219	9
TACGGAT	55	1.9563656E-4	13.8192005	16
TCGACTC	50	0.001497196	13.300981	13
AATCGCT	50	0.001497196	13.300981	15
TAATTCC	50	0.0014996941	13.2980385	4
AAGACGG	110	2.733941E-9	12.954546	5
TCAGGAC	110	2.739398E-9	12.952635	3
GGTATCA	385	0.0	12.82266	1
TGCGCTA	75	1.4723219E-5	12.66947	10
GCGCTAT	75	1.4723219E-5	12.66947	11
CGAGCGG	60	4.0816626E-4	12.668534	6
GCTATCG	75	1.47455685E-5	12.667601	13
>>END_MODULE