FastQCFastQC Report
Thu 2 Feb 2017
SRR4062118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679486
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAA7780.11449831195933396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG250.002352243835.199474
GGTATCA5000.026.4034881
GCTATCG609.745912E-625.6662813
GTATCAA11200.025.539091
GCGCTAT651.8120862E-523.69195211
TGCGCTA703.213054E-521.99966810
CGCAATA1407.2759576E-1221.99966836
ATACGAA1500.021.99966840
TATCGGT703.213054E-521.99966815
CGCTATC755.468748E-520.53302412
GCAATAC1553.45608E-1119.87066837
CAATACG1553.45608E-1119.87066838
AATACGA1553.45608E-1119.87066839
TCTAGCG1605.2750693E-1119.25112528
CTATCGG808.982232E-519.2497114
TACGAAT1658.185452E-1118.66638641
GTATAGG951.5936168E-518.5287651
TCCCGAC951.595639E-518.5260372
ACGGCCC600.007406343718.33440625
GCGCAAT1701.2551027E-1018.11737435
Produced by FastQC (version 0.11.5)