##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679486
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260574905148893	32.0	32.0	32.0	32.0	32.0
2	31.428204554619228	32.0	32.0	32.0	32.0	32.0
3	31.555206435452682	32.0	32.0	32.0	32.0	32.0
4	31.63285777779086	32.0	32.0	32.0	32.0	32.0
5	31.601740138869676	32.0	32.0	32.0	32.0	32.0
6	35.23167511913417	36.0	36.0	36.0	36.0	36.0
7	35.23498644563685	36.0	36.0	36.0	36.0	36.0
8	35.198764948799536	36.0	36.0	36.0	36.0	36.0
9	35.28339215230336	36.0	36.0	36.0	36.0	36.0
10	35.15508487297752	36.0	36.0	36.0	36.0	36.0
11	35.27750240623059	36.0	36.0	36.0	36.0	36.0
12	35.202709106589396	36.0	36.0	36.0	36.0	36.0
13	35.223420350088155	36.0	36.0	36.0	36.0	36.0
14	35.20545530003562	36.0	36.0	36.0	36.0	36.0
15	35.17176512834701	36.0	36.0	36.0	36.0	36.0
16	35.18622017230671	36.0	36.0	36.0	36.0	36.0
17	35.17079822100823	36.0	36.0	36.0	36.0	36.0
18	35.16092605292824	36.0	36.0	36.0	36.0	36.0
19	35.1553144582817	36.0	36.0	36.0	36.0	36.0
20	35.15004282648943	36.0	36.0	36.0	36.0	36.0
21	35.1401544697021	36.0	36.0	36.0	36.0	36.0
22	35.13612642497417	36.0	36.0	36.0	36.0	36.0
23	35.1091884159497	36.0	36.0	36.0	36.0	36.0
24	35.08646682933865	36.0	36.0	36.0	36.0	36.0
25	35.0615288615218	36.0	36.0	36.0	36.0	36.0
26	35.02354279558372	36.0	36.0	36.0	36.0	36.0
27	35.015680970616025	36.0	36.0	36.0	36.0	36.0
28	34.993518630258755	36.0	36.0	36.0	36.0	36.0
29	34.97538433462941	36.0	36.0	36.0	36.0	36.0
30	34.96254521800302	36.0	36.0	36.0	36.0	36.0
31	34.9485993824744	36.0	36.0	36.0	36.0	36.0
32	34.92223239330906	36.0	36.0	36.0	32.0	36.0
33	34.8989706925529	36.0	36.0	36.0	32.0	36.0
34	34.87853465707903	36.0	36.0	36.0	32.0	36.0
35	34.86385738631848	36.0	36.0	36.0	32.0	36.0
36	34.85404114286387	36.0	36.0	36.0	32.0	36.0
37	34.8315947642777	36.0	36.0	36.0	32.0	36.0
38	34.82512369644113	36.0	36.0	36.0	32.0	36.0
39	34.79836965000015	36.0	36.0	36.0	32.0	36.0
40	34.79223707331718	36.0	36.0	36.0	32.0	36.0
41	34.77426760816264	36.0	36.0	36.0	32.0	36.0
42	34.737101279496564	36.0	36.0	36.0	32.0	36.0
43	34.72629016639048	36.0	36.0	36.0	32.0	36.0
44	34.67538256858861	36.0	36.0	36.0	32.0	36.0
45	34.66052722204725	36.0	36.0	36.0	32.0	36.0
46	34.64238262451323	36.0	36.0	36.0	32.0	36.0
47	34.60855116956052	36.0	36.0	36.0	32.0	36.0
48	34.58378539072181	36.0	36.0	36.0	32.0	36.0
49	34.566020491960096	36.0	36.0	36.0	32.0	36.0
50	34.167385347159474	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	14.0
22	43.0
23	149.0
24	333.0
25	772.0
26	1660.0
27	3114.0
28	5604.0
29	8755.0
30	13298.0
31	19421.0
32	29638.0
33	49552.0
34	112405.0
35	434722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.403645185461066	18.359004102710557	12.575904561560531	25.661446150267846
2	15.982444831027085	19.8315421998122	37.731692025114135	26.45432094404658
3	18.2609156359826	23.446173861341844	29.26882752206086	29.0240829806147
4	12.439844235201313	16.170899768354317	35.83561692220296	35.55363907424141
5	14.27963825544346	36.797280293163205	33.77925929196376	15.143822159429568
6	33.951742864080884	35.85450745785411	16.692642221682597	13.501107456382409
7	29.92114627821618	31.039785955854864	20.321537161913565	18.71753060401539
8	28.322732182855866	33.20524631854078	19.021436791928018	19.45058470667534
9	27.157881104246446	14.18748877828241	19.03276888707052	39.621861230400626
10	16.466711602593726	26.896507065634907	31.318231722213557	25.31854960955781
11	36.83239978454302	21.688747082353427	21.67991687834628	19.798936254757272
12	23.830954574487187	24.15193249014696	29.122454325769777	22.89465860959608
13	29.369552867903092	20.213808673026374	25.06600577495342	25.350632684117112
14	23.234621463871218	19.570233970972176	25.605089729589718	31.59005483556688
15	25.37653461587141	27.013360098662815	22.51996361955949	25.09014166590629
16	25.53433850636879	26.163932978652948	23.7996423762114	24.50208613876686
17	23.251251681418015	26.325781546639664	25.670874749442962	24.752092022499358
18	24.210577438173672	25.695674953346952	25.944245927792263	24.14950168068711
19	25.559172668752556	25.191541841921687	25.452327200266083	23.79695828905967
20	25.9931830423764	24.659078533353348	25.152984185011523	24.194754239258728
21	26.329313628242524	24.287623291723452	25.170349352304537	24.21271372772949
22	25.674259660979033	24.451865086256376	25.514580138516468	24.359295114248123
23	24.433946297563597	24.797456897503256	25.715652295488496	25.052944509444654
24	24.918430390253736	25.24147106394439	25.457959825219547	24.38213872058233
25	24.766551137994217	24.966702723386096	25.513587496412725	24.753158642206966
26	24.825822175258068	25.134294267069723	25.659108904188216	24.380774653483996
27	24.658122928575214	25.083592843981613	25.510387293887714	24.747896933555463
28	24.23768350564774	25.165311453695864	25.816991059273704	24.780013981382684
29	24.638877728377185	25.26170786735131	25.543247105502893	24.556167298768617
30	24.560616005910454	25.493985053144048	25.821738580136987	24.123660360808508
31	25.069133240616054	25.05868415049633	24.917989359588514	24.9541932492991
32	24.6067557941708	24.830604760138694	25.067993571516872	25.494645874173628
33	24.700469917481136	24.71710025740234	25.635742574111713	24.94668725100481
34	25.00371608246907	25.141615855677657	25.829201491142477	24.025466570710794
35	25.206407233745708	24.780494553203763	25.695603415543015	24.317494797507514
36	24.753158642206966	24.993487714960594	25.255745159937305	24.997608482895135
37	25.1798815261783	25.02064093601678	25.067000257551786	24.732477280253136
38	24.71618252620363	24.967990510474095	25.64158790615259	24.674239057169682
39	25.400158945075646	24.757314416907047	25.17027727085418	24.672249367163126
40	25.069611646484685	24.902720299521402	25.51509674988668	24.512571304107233
41	24.408848544252287	25.47922746692687	25.581658888648505	24.53026510017234
42	25.42354172277831	25.34406555900443	25.063544136498837	24.16884858171842
43	24.991463809495777	24.724192741294555	25.342036322962354	24.942307126247314
44	25.034733464810287	25.025314220115973	24.943337356135757	24.99661495893798
45	24.955149196070494	25.210493395636334	24.926009051105634	24.908348357187535
46	25.031127385329064	25.22112953407529	24.703517173780586	25.04422590681506
47	24.83237181373992	25.20648142697357	25.403985400600458	24.557161358686052
48	25.247696700320844	25.323639360668764	24.837223677626348	24.59144026138404
49	24.84888712931906	25.642901190505974	25.206671778576776	24.301539901598183
50	24.476985908237978	25.89087162883108	25.095846057618015	24.536296405312928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	293.0
1	165.5
2	38.0
3	87.5
4	137.0
5	143.0
6	149.0
7	120.0
8	91.0
9	105.5
10	120.0
11	252.0
12	384.0
13	690.0
14	996.0
15	1288.0
16	1580.0
17	1652.0
18	1724.0
19	1682.5
20	1641.0
21	1913.0
22	2185.0
23	2649.5
24	3114.0
25	3726.5
26	4339.0
27	5549.0
28	6759.0
29	7877.0
30	8995.0
31	10332.5
32	11670.0
33	13319.5
34	14969.0
35	17155.5
36	19342.0
37	22722.0
38	26102.0
39	28959.5
40	31817.0
41	34888.5
42	37960.0
43	38982.0
44	40004.0
45	42459.5
46	44915.0
47	47276.0
48	49637.0
49	51337.5
50	53038.0
51	51502.5
52	49967.0
53	49227.0
54	48487.0
55	47870.0
56	47253.0
57	45413.0
58	43573.0
59	39624.0
60	35675.0
61	31312.5
62	26950.0
63	23143.5
64	19337.0
65	16458.0
66	13579.0
67	12186.0
68	10793.0
69	9372.5
70	7952.0
71	6506.0
72	5060.0
73	4546.0
74	4032.0
75	2995.0
76	1958.0
77	1572.5
78	1187.0
79	978.5
80	770.0
81	595.0
82	420.0
83	332.0
84	244.0
85	189.0
86	134.0
87	93.5
88	53.0
89	38.0
90	23.0
91	16.0
92	9.0
93	9.0
94	9.0
95	9.5
96	10.0
97	8.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02634344195465396
2	0.004120761870001737
3	2.943401335715526E-4
4	0.0
5	1.471700667857763E-4
6	1.471700667857763E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.471700667857763E-4
17	0.0
18	2.943401335715526E-4
19	0.0
20	5.886802671431052E-4
21	0.0
22	0.0
23	1.471700667857763E-4
24	7.358503339288815E-4
25	1.471700667857763E-4
26	0.0011773605342862105
27	2.943401335715526E-4
28	0.0016188707346435393
29	0.0010301904675004343
30	0.0017660408014293155
31	1.471700667857763E-4
32	0.001471700667857763
33	7.358503339288815E-4
34	0.0010301904675004343
35	5.886802671431052E-4
36	1.471700667857763E-4
37	0.0016188707346435393
38	0.0
39	8.830204007146577E-4
40	2.943401335715526E-4
41	0.0010301904675004343
42	0.0054452924710737234
43	0.003826421736430184
44	0.003826421736430184
45	0.0016188707346435393
46	0.002943401335715526
47	8.830204007146577E-4
48	0.003826421736430184
49	0.00397359180321596
50	0.0016188707346435393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	679486.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.57574921386757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26568483355715	49.601096154101384
2	13.57048584229719	16.983666375558588
3	3.6885804609053747	6.9244705763036025
4	1.3514072346284904	3.3826128079967486
5	0.5918413511369524	1.8517457981571233
6	0.31960351736461856	1.1999657730287003
7	0.21965717631814058	0.9621648664817178
8	0.13510607425636434	0.6763491055949139
9	0.1062137064534884	0.5981762032297954
>10	0.6183664512216985	7.503326762738058
>50	0.0784614601332924	3.4069728936978585
>100	0.05341024904923675	6.474229035882742
>500	0.0011816426780147152	0.435223647228807
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAA	778	0.11449831195933396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.471700667857763E-4	1.471700667857763E-4
13	0.0	0.0	0.0	1.471700667857763E-4	1.471700667857763E-4
14	0.0	0.0	0.0	1.471700667857763E-4	1.471700667857763E-4
15	0.0	0.0	0.0	1.471700667857763E-4	2.943401335715526E-4
16	0.0	0.0	0.0	1.471700667857763E-4	2.943401335715526E-4
17	0.0	0.0	0.0	1.471700667857763E-4	2.943401335715526E-4
18	0.0	0.0	0.0	1.471700667857763E-4	2.943401335715526E-4
19	0.0	0.0	0.0	1.471700667857763E-4	2.943401335715526E-4
20	0.0	0.0	0.0	1.471700667857763E-4	2.943401335715526E-4
21	0.0	0.0	0.0	1.471700667857763E-4	4.415102003573289E-4
22	0.0	0.0	0.0	1.471700667857763E-4	5.886802671431052E-4
23	0.0	0.0	0.0	2.943401335715526E-4	5.886802671431052E-4
24	0.0	0.0	0.0	8.830204007146578E-4	5.886802671431052E-4
25	0.0	0.0	0.0	0.0013245306010719869	5.886802671431052E-4
26	0.0	0.0	0.0	0.0016188707346435395	5.886802671431052E-4
27	0.0	0.0	0.0	0.001913210868215092	5.886802671431052E-4
28	0.0	0.0	0.0	0.0026490612021439737	5.886802671431052E-4
29	0.0	0.0	0.0	0.004415102003573289	5.886802671431052E-4
30	0.0	0.0	0.0	0.00794718360643192	5.886802671431052E-4
31	0.0	0.0	0.0	0.011773605342862105	5.886802671431052E-4
32	0.0	0.0	0.0	0.016041537279649618	5.886802671431052E-4
33	0.0	0.0	0.0	0.022958530418581105	7.358503339288815E-4
34	0.0	0.0	0.0	0.028403822889654828	7.358503339288815E-4
35	0.0	0.0	0.0	0.035615156162157866	7.358503339288815E-4
36	0.0	0.0	0.0	0.04444536016930444	7.358503339288815E-4
37	0.0	0.0	0.0	0.06004538724859673	7.358503339288815E-4
38	0.0	0.0	0.0	0.07917749593074766	7.358503339288815E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACACG	25	0.0023522438	35.19947	4
GGTATCA	500	0.0	26.403488	1
GCTATCG	60	9.745912E-6	25.66628	13
GTATCAA	1120	0.0	25.53909	1
GCGCTAT	65	1.8120862E-5	23.691952	11
TGCGCTA	70	3.213054E-5	21.999668	10
CGCAATA	140	7.2759576E-12	21.999668	36
ATACGAA	150	0.0	21.999668	40
TATCGGT	70	3.213054E-5	21.999668	15
CGCTATC	75	5.468748E-5	20.533024	12
GCAATAC	155	3.45608E-11	19.870668	37
CAATACG	155	3.45608E-11	19.870668	38
AATACGA	155	3.45608E-11	19.870668	39
TCTAGCG	160	5.2750693E-11	19.251125	28
CTATCGG	80	8.982232E-5	19.24971	14
TACGAAT	165	8.185452E-11	18.666386	41
GTATAGG	95	1.5936168E-5	18.528765	1
TCCCGAC	95	1.595639E-5	18.526037	2
ACGGCCC	60	0.0074063437	18.334406	25
GCGCAAT	170	1.2551027E-10	18.117374	35
>>END_MODULE