FastQCFastQC Report
Thu 2 Feb 2017
SRR4062117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259452
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41290.18274342628212503No Hit
TATCAACGCAGAGTACTTTTTTTTT24770.10962835236154607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.013.9954781
GTATCAA17150.012.6810341
CGATACC604.0929776E-412.6667844
ACCGTGC1950.011.6931948
CAGTTCG752.0727189E-411.4011179
GTAATAC1658.0035534E-1110.9392533
CCGCGCG700.00149063610.8582079
TGCGCAA1002.3970966E-510.45102510
CCGATCA1201.5224578E-610.2926759
CCGATAC750.002648645510.13320353
GTGTACG1252.5815607E-69.8768081
TGGACCG1356.4425694E-79.8521625
GACCGTG2201.6370905E-119.500937
TACACCG1201.7002847E-59.50029855
ATAGGAC1901.0531949E-99.4998783
CAGGACT5450.09.4129314
CCTAGAC2850.09.3332143
TAGGACA2750.09.327364
GTCTAGA2501.8189894E-129.1170541
GAATAAG2501.8189894E-129.1170541
Produced by FastQC (version 0.11.5)