##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062117_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2259452
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226264156087407	32.0	32.0	32.0	32.0	32.0
2	30.795958489049557	32.0	32.0	32.0	32.0	32.0
3	30.82149122884664	32.0	32.0	32.0	32.0	32.0
4	30.836575417402095	32.0	32.0	32.0	32.0	32.0
5	30.762518522190337	32.0	32.0	32.0	32.0	32.0
6	34.376719664768274	36.0	36.0	36.0	32.0	36.0
7	34.32220025032618	36.0	36.0	36.0	32.0	36.0
8	34.28432867792721	36.0	36.0	36.0	32.0	36.0
9	34.41322763218692	36.0	36.0	36.0	32.0	36.0
10	34.133720034769496	36.0	36.0	36.0	32.0	36.0
11	34.38613522216892	36.0	36.0	36.0	32.0	36.0
12	34.239592609181344	36.0	36.0	36.0	32.0	36.0
13	34.29869145261772	36.0	36.0	36.0	32.0	36.0
14	34.23131139763093	36.0	36.0	36.0	32.0	36.0
15	34.197631549596984	36.0	36.0	36.0	32.0	36.0
16	34.19018903698773	36.0	36.0	36.0	32.0	36.0
17	34.14004767527702	36.0	36.0	36.0	32.0	36.0
18	34.12089568621064	36.0	36.0	36.0	32.0	36.0
19	34.134770289433014	36.0	36.0	36.0	32.0	36.0
20	34.11508454262361	36.0	36.0	36.0	32.0	36.0
21	34.10414649215828	36.0	36.0	36.0	32.0	36.0
22	34.07903642122072	36.0	36.0	36.0	32.0	36.0
23	34.04514900073115	36.0	36.0	36.0	32.0	36.0
24	34.01594722968224	36.0	36.0	36.0	32.0	36.0
25	33.662495596277324	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	30.0
5	160.0
6	482.0
7	90.0
8	359.0
9	304.0
10	158.0
11	48.0
12	102.0
13	96.0
14	286.0
15	475.0
16	743.0
17	1037.0
18	1420.0
19	1889.0
20	2873.0
21	4141.0
22	5964.0
23	9016.0
24	12839.0
25	18310.0
26	25285.0
27	33264.0
28	44503.0
29	59301.0
30	78869.0
31	108131.0
32	153940.0
33	218594.0
34	473789.0
35	1002949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.432979246508076	16.642715166278883	11.577979726718322	26.34632586049472
2	16.63089093521155	18.661521452603868	37.51623185506536	27.191355757119222
3	19.50192813860823	21.791573372611627	27.98919020833988	30.71730828044027
4	13.305264723405271	14.976899168122179	34.308785398185044	37.4090507102875
5	15.183169116816936	35.78488558709526	33.689437088951415	15.34250820713639
6	36.14156898226203	33.81352918486279	15.91413249650028	14.130769336374913
7	31.23223945665032	29.863010664079287	19.618450141995737	19.286299737274657
8	28.168876802588805	33.74235357655683	18.48317820070967	19.60559142014469
9	26.792795100079626	14.863307446253698	18.100691538172846	40.24320591549383
10	15.806490925246008	27.456786621966867	31.878078558497876	24.858643894289255
11	36.90188222676245	21.30116071389001	21.740849240151217	20.05610781919632
12	24.333847394683897	23.15553901105232	28.927295771402235	23.58331782286155
13	29.782127603758152	19.873378231903864	24.41862732325287	25.925866841085117
14	23.015732352391225	19.0130348206682	25.36424210790304	32.60699071903754
15	24.976734416047137	27.0977136799861	21.954557218269287	25.970994685697473
16	25.47872755244557	26.185209605036476	23.373726524448603	24.96233631806935
17	24.212927534253918	26.061870416249217	25.057726845121014	24.66747520437585
18	24.58006202924236	25.84940186087727	25.331058927780237	24.23947718210013
19	25.355724124915774	25.2406307189528	25.24470638998767	24.158938766143756
20	25.373534997269005	24.99646720436038	25.110889914544	24.519107883826617
21	25.382580398806788	24.690865823762735	25.14988256578044	24.776671211650044
22	25.282960689419298	24.588960501576288	25.12652821809637	25.001550590908046
23	24.85677382090327	24.82045235287812	25.11168851417733	25.211085312041277
24	24.738256597351267	24.838590645500094	25.28382575276007	25.139327004388562
25	24.877142748299224	24.86403088729204	25.12077530079761	25.138051063611133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	340.0
1	340.0
2	464.0
3	588.0
4	588.0
5	588.0
6	1353.5
7	2119.0
8	2119.0
9	2119.0
10	2814.0
11	3509.0
12	3509.0
13	3509.0
14	5023.5
15	6538.0
16	6538.0
17	6538.0
18	10668.5
19	14799.0
20	14799.0
21	14799.0
22	22369.5
23	29940.0
24	29940.0
25	29940.0
26	42226.5
27	54513.0
28	54513.0
29	54513.0
30	70788.5
31	87064.0
32	87064.0
33	87064.0
34	107559.5
35	128055.0
36	128055.0
37	128055.0
38	153032.0
39	178009.0
40	178009.0
41	178009.0
42	206260.0
43	234511.0
44	234511.0
45	234511.0
46	260577.5
47	286644.0
48	286644.0
49	286644.0
50	300111.5
51	313579.0
52	313579.0
53	313579.0
54	305160.5
55	296742.0
56	296742.0
57	296742.0
58	271628.5
59	246515.0
60	246515.0
61	246515.0
62	210659.5
63	174804.0
64	174804.0
65	174804.0
66	140760.0
67	106716.0
68	106716.0
69	106716.0
70	80397.0
71	54078.0
72	54078.0
73	54078.0
74	38974.0
75	23870.0
76	23870.0
77	23870.0
78	16938.0
79	10006.0
80	10006.0
81	10006.0
82	6877.5
83	3749.0
84	3749.0
85	3749.0
86	2541.5
87	1334.0
88	1334.0
89	1334.0
90	870.0
91	406.0
92	406.0
93	406.0
94	293.0
95	180.0
96	180.0
97	180.0
98	512.0
99	844.0
100	844.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001106462983059609
2	2.655511159343062E-4
3	8.409118671253029E-4
4	0.010002425366858867
5	0.03306111393382112
6	0.06262580484117387
7	0.10365345225302419
8	0.10405177892692564
9	0.11502789171887697
10	0.12910210086339519
11	0.13640475655158862
12	0.12888080826678328
13	0.12436643929590006
14	0.11392142873581737
15	0.12834970603491466
16	0.11666545693380517
17	0.11963077772840494
18	0.10852188937848647
19	0.0953771091397383
20	0.08997756978240741
21	0.08931369199257165
22	0.09949315143672006
23	0.08117012443725292
24	0.08820722900951204
25	0.08648114675593904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2259452.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.83687853159249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.64022885308539	36.95375663144268
2	16.07191537599501	17.30523511734954
3	6.47011486288078	10.449923638750914
4	3.1095421449485228	6.696321709858448
5	1.7769626662796585	4.783306160983635
6	1.0585395702912601	3.4193079759992804
7	0.7097943845397358	2.674917984401054
8	0.462849355054721	1.9934691605205552
9	0.33435900245811756	1.6200760501153901
>10	1.3235203434356513	11.355942881923012
>50	0.030596938398293416	1.0982535922401009
>100	0.010833358486462578	1.0824788770857527
>500	4.1285785914434766E-4	0.14785386040701848
>1k	3.302862873151217E-4	0.4191563589226048
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4129	0.18274342628212503	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2477	0.10962835236154607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.851703864476873E-5	0.0	0.0	0.0	0.0
8	8.851703864476873E-5	0.0	0.0	0.0	0.0
9	8.851703864476873E-5	0.0	0.0	0.0	0.0
10	8.851703864476873E-5	0.0	0.0	0.0	0.0
11	8.851703864476873E-5	0.0	0.0	0.0	0.0
12	8.851703864476873E-5	0.0	0.0	0.0	0.0
13	1.327755579671531E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	570	0.0	13.995478	1
GTATCAA	1715	0.0	12.681034	1
CGATACC	60	4.0929776E-4	12.666784	4
ACCGTGC	195	0.0	11.693194	8
CAGTTCG	75	2.0727189E-4	11.401117	9
GTAATAC	165	8.0035534E-11	10.939253	3
CCGCGCG	70	0.001490636	10.858207	9
TGCGCAA	100	2.3970966E-5	10.451025	10
CCGATCA	120	1.5224578E-6	10.292675	9
CCGATAC	75	0.0026486455	10.1332035	3
GTGTACG	125	2.5815607E-6	9.876808	1
TGGACCG	135	6.4425694E-7	9.852162	5
GACCGTG	220	1.6370905E-11	9.50093	7
TACACCG	120	1.7002847E-5	9.5002985	5
ATAGGAC	190	1.0531949E-9	9.499878	3
CAGGACT	545	0.0	9.412931	4
CCTAGAC	285	0.0	9.333214	3
TAGGACA	275	0.0	9.32736	4
GTCTAGA	250	1.8189894E-12	9.117054	1
GAATAAG	250	1.8189894E-12	9.117054	1
>>END_MODULE