FastQCFastQC Report
Thu 2 Feb 2017
SRR4062117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259452
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20150.025.6632861
GGTATCA8900.022.4993951
TCAACGC27900.018.3723814
ATCAACG28600.017.8461823
CAACGCA28750.017.8291975
TATCAAC29200.017.7812422
AACGCAG30800.016.8567946
TAGTACC1805.2750693E-915.888564
ATAGTAC2203.0559022E-1015.0000223
GTATAGA3450.014.6699361
GTAGGAC3750.014.6666893
GTAATAC3000.014.6666883
ACGCAGA36350.014.1014987
CGCAGAG37200.013.7792868
GTATTAC2602.564775E-1013.5414781
GTCCTAA2309.129508E-913.3942881
TATACTG4450.013.348045
AGAGTAC39000.013.25613711
TAACGCA1000.008764775513.1997284
TAGTACT2552.7921487E-912.9409094
Produced by FastQC (version 0.11.5)