##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2259452
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230928118853598	32.0	32.0	32.0	32.0	32.0
2	31.360613547001662	32.0	32.0	32.0	32.0	32.0
3	31.513572760120596	32.0	32.0	32.0	32.0	32.0
4	31.601954367696237	32.0	32.0	32.0	32.0	32.0
5	31.56549198655249	32.0	32.0	32.0	32.0	32.0
6	35.189885866130375	36.0	36.0	36.0	36.0	36.0
7	35.19309637912202	36.0	36.0	36.0	36.0	36.0
8	35.13614318870239	36.0	36.0	36.0	36.0	36.0
9	35.23999093585524	36.0	36.0	36.0	36.0	36.0
10	35.09570240925676	36.0	36.0	36.0	36.0	36.0
11	35.23923942619715	36.0	36.0	36.0	36.0	36.0
12	35.163219223068246	36.0	36.0	36.0	36.0	36.0
13	35.19303264685419	36.0	36.0	36.0	36.0	36.0
14	35.15756475463962	36.0	36.0	36.0	36.0	36.0
15	35.12866482669249	36.0	36.0	36.0	36.0	36.0
16	35.15030016127805	36.0	36.0	36.0	36.0	36.0
17	35.136746432320756	36.0	36.0	36.0	36.0	36.0
18	35.125516275627895	36.0	36.0	36.0	36.0	36.0
19	35.12130242200321	36.0	36.0	36.0	36.0	36.0
20	35.119513050067006	36.0	36.0	36.0	36.0	36.0
21	35.11248833788016	36.0	36.0	36.0	36.0	36.0
22	35.09189042298752	36.0	36.0	36.0	36.0	36.0
23	35.06331579515741	36.0	36.0	36.0	36.0	36.0
24	35.05080169881901	36.0	36.0	36.0	36.0	36.0
25	35.03808268553614	36.0	36.0	36.0	36.0	36.0
26	34.989364235221636	36.0	36.0	36.0	36.0	36.0
27	34.97908563669421	36.0	36.0	36.0	36.0	36.0
28	34.960744021116625	36.0	36.0	36.0	36.0	36.0
29	34.9524636947366	36.0	36.0	36.0	36.0	36.0
30	34.924394941782346	36.0	36.0	36.0	32.0	36.0
31	34.92175580627515	36.0	36.0	36.0	32.0	36.0
32	34.896816130636985	36.0	36.0	36.0	32.0	36.0
33	34.88167794668796	36.0	36.0	36.0	32.0	36.0
34	34.872741266466385	36.0	36.0	36.0	32.0	36.0
35	34.85674623758327	36.0	36.0	36.0	32.0	36.0
36	34.83221285515249	36.0	36.0	36.0	32.0	36.0
37	34.82224937728263	36.0	36.0	36.0	32.0	36.0
38	34.809035996338935	36.0	36.0	36.0	32.0	36.0
39	34.7951454600496	36.0	36.0	36.0	32.0	36.0
40	34.78426892892613	36.0	36.0	36.0	32.0	36.0
41	34.75556418104921	36.0	36.0	36.0	32.0	36.0
42	34.73662419029039	36.0	36.0	36.0	32.0	36.0
43	34.72599594946031	36.0	36.0	36.0	32.0	36.0
44	34.69900002301443	36.0	36.0	36.0	32.0	36.0
45	34.67944395366664	36.0	36.0	36.0	32.0	36.0
46	34.65930765513053	36.0	36.0	36.0	32.0	36.0
47	34.6325241695774	36.0	36.0	36.0	32.0	36.0
48	34.61188155358025	36.0	36.0	36.0	32.0	36.0
49	34.59164921405721	36.0	36.0	36.0	32.0	36.0
50	34.16052520699709	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	18.0
21	48.0
22	159.0
23	410.0
24	1145.0
25	2602.0
26	5640.0
27	10651.0
28	18198.0
29	29436.0
30	45023.0
31	66751.0
32	101059.0
33	172787.0
34	387565.0
35	1417958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96902732245276	17.117008630822596	12.07035703138662	26.843607015338026
2	16.153474025528546	19.078869039056755	37.13089543776471	27.636761497649992
3	18.962623647347808	22.207882449268627	28.19686206820244	30.63263183518113
4	13.089988191827045	15.429582040246927	34.433437842450296	37.04699192547574
5	14.982312074924394	36.073143431745144	33.40032600839761	15.544218484932845
6	36.302849191483936	34.054777981170616	15.765178043486728	13.877194783858712
7	31.43695905024758	29.736590996400896	19.60125729601691	19.22519265733461
8	28.27530746393373	33.844047140634096	18.294568771542835	19.58607662388933
9	26.930689388400374	14.634079413946392	17.945723122243802	40.48950807540944
10	15.739126124387685	27.478742633169457	31.942347082389887	24.839784160052968
11	37.275941245930426	21.14862364856611	21.590456446961475	19.984978658541984
12	24.44359271345119	23.02479673159542	28.937073651962358	23.594536902991038
13	29.96009651897894	19.740892924479034	24.397376000906416	25.901634555635617
14	23.1103825175308	18.75684900586514	25.321449625838476	32.81131885076559
15	25.15459500799309	27.043725646749746	21.903319920051413	25.898359425205758
16	25.669933403881757	26.044716370111015	23.3957425752653	24.889607650741933
17	24.41972655316422	25.92916335465414	25.053242998744828	24.597867093436815
18	24.71257372925877	25.72897197502577	25.329139980588188	24.229314315127272
19	25.549436566670398	25.06909864387411	25.26171180521286	24.11975298424263
20	25.62925962660676	24.81516445881968	25.049071872202017	24.506504042371542
21	25.496359294200538	24.637301434153063	25.126889174897276	24.739450096749124
22	25.407178377765938	24.51886563644636	25.121799445175203	24.952156540612503
23	25.033647554206752	24.627885269474753	25.02488436350246	25.313582812816033
24	24.903969185162104	24.69940440756987	25.24688738005277	25.149739027215258
25	25.09780056606822	24.591481536394998	25.18237885852499	25.12833903901179
26	24.97154162093262	24.86474524660978	25.064352228870874	25.09936090358673
27	24.982739116348977	25.067361704915015	24.934231607422003	25.015667571314005
28	25.23954166978325	24.853159152861863	25.007667900464675	24.89963127689021
29	25.098309801680855	25.10556824061194	24.896489792377665	24.899632165329535
30	25.154231991293347	24.975159565216043	24.972415499369085	24.898192944121526
31	25.038925540022717	24.755050140698458	24.945849461946796	25.26017485733203
32	24.925224503525225	24.99329476903467	25.016840530576296	25.064640196863813
33	25.048352560760467	24.956250337472106	24.986611762352364	25.008785339415063
34	25.200891908734263	24.80353399804552	25.06452972084921	24.931044372371012
35	25.165571413902864	24.962070381792365	25.082498026066546	24.789860178238225
36	25.21056083758733	24.927172284130332	25.020735208073496	24.841531670208838
37	25.358732873301935	24.571492304852903	25.060690595911943	25.009084225933222
38	25.10984964495816	24.902985325645332	25.05886383069877	24.928301198697735
39	25.207507720960205	24.948150731554893	24.852507309352696	24.991834238132206
40	25.311945246876345	24.76637023772589	25.015767104486887	24.90591741091088
41	25.087389606722425	24.943901581909	25.035208119598728	24.93350069176984
42	25.168964525195296	24.847456126762122	24.954787881470335	25.028791466572244
43	25.14359212003801	24.612953011727704	25.09061230415283	25.152842564081457
44	25.02572641435527	24.885199333790986	25.093710538999915	24.995363712853823
45	25.09052202027534	24.9174225843644	25.005145182670578	24.98691021268968
46	25.277640572967286	24.789058668776963	24.880764593723278	25.05253616453247
47	25.15884495068681	25.058465918043265	24.920865207069376	24.86182392420055
48	25.124935103676993	25.010567090958418	24.798074510052125	25.06642329531246
49	25.02963210163523	24.933056678531308	24.94217424826523	25.095136971568234
50	25.07403458502791	25.14458375766846	24.863626324843977	24.917755332459656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	126.0
2	70.0
3	184.5
4	299.0
5	319.5
6	340.0
7	255.5
8	171.0
9	289.5
10	408.0
11	643.5
12	879.0
13	1668.0
14	2457.0
15	3233.0
16	4009.0
17	3889.0
18	3769.0
19	3906.0
20	4043.0
21	4820.5
22	5598.0
23	7404.5
24	9211.0
25	11475.5
26	13740.0
27	16520.0
28	19300.0
29	22745.0
30	26190.0
31	30558.5
32	34927.0
33	40666.0
34	46405.0
35	53516.0
36	60627.0
37	67114.0
38	73601.0
39	81535.5
40	89470.0
41	98157.5
42	106845.0
43	115749.0
44	124653.0
45	135299.0
46	145945.0
47	153583.5
48	161222.0
49	168419.0
50	175616.0
51	179756.5
52	183897.0
53	183829.0
54	183761.0
55	177904.0
56	172047.0
57	163598.0
58	155149.0
59	144087.0
60	133025.0
61	117568.0
62	102111.0
63	89444.5
64	76778.0
65	65079.5
66	53381.0
67	43658.0
68	33935.0
69	27662.0
70	21389.0
71	17238.5
72	13088.0
73	10902.0
74	8716.0
75	6949.0
76	5182.0
77	4239.5
78	3297.0
79	2639.0
80	1981.0
81	1448.0
82	915.0
83	675.0
84	435.0
85	335.5
86	236.0
87	153.0
88	70.0
89	49.0
90	28.0
91	20.5
92	13.0
93	10.5
94	8.0
95	10.5
96	13.0
97	13.0
98	13.0
99	10.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03040560277447806
2	0.0043815934129160525
3	8.851703864476873E-5
4	0.0
5	4.425851932238437E-5
6	1.7703407728953747E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	4.425851932238437E-5
13	0.0
14	0.0
15	0.0
16	4.86843712546228E-4
17	0.0
18	1.327755579671531E-4
19	4.425851932238437E-5
20	4.86843712546228E-4
21	0.0
22	0.0
23	4.425851932238437E-5
24	9.294289057700716E-4
25	3.0980963525669054E-4
26	5.311022318686124E-4
27	3.5406815457907494E-4
28	0.001460531137638684
29	5.753607511909968E-4
30	0.0013720140989939155
31	4.425851932238436E-4
32	9.73687425092456E-4
33	2.655511159343062E-4
34	0.0012392385410267622
35	1.7703407728953747E-4
36	4.425851932238436E-4
37	0.001814599292217759
38	0.0
39	7.966533478029185E-4
40	4.425851932238437E-5
41	0.001327755579671531
42	0.004514368970883205
43	0.004470110451560821
44	0.004470110451560821
45	0.002522735601375909
46	0.0024784770820535243
47	7.081363091581499E-4
48	0.003496423026468365
49	0.0034521645071459808
50	0.0015490481762834526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2259452.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.05886334673886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.64805767603974	36.56977105581341
2	16.61472267714103	17.963460454950607
3	6.757485731772466	10.959059932242761
4	3.3093145090187743	7.155911232577044
5	1.751749708005574	4.73487990413815
6	1.047129537682327	3.3963979550341654
7	0.7150260827575678	2.7057448108001743
8	0.4718808454150065	2.040747371058675
9	0.33832599455150714	1.6460566835498496
>10	1.313068732668601	11.155435695353702
>50	0.026227513417543303	0.9285849395071105
>100	0.006599307673588417	0.5685792937361102
>500	3.293470849646702E-4	0.1104746208156759
>1k	8.233677124083279E-5	0.06489605042253183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.425851932238437E-5	0.0	0.0	0.0	0.0
6	4.425851932238437E-5	0.0	0.0	0.0	0.0
7	4.425851932238437E-5	0.0	0.0	0.0	0.0
8	4.425851932238437E-5	0.0	0.0	0.0	0.0
9	4.425851932238437E-5	0.0	0.0	0.0	0.0
10	4.425851932238437E-5	0.0	0.0	0.0	0.0
11	4.425851932238437E-5	0.0	0.0	0.0	0.0
12	4.425851932238437E-5	0.0	0.0	0.0	0.0
13	4.425851932238437E-5	0.0	0.0	0.0	0.0
14	8.851703864476873E-5	0.0	0.0	0.0	0.0
15	8.851703864476873E-5	0.0	0.0	0.0	4.425851932238437E-5
16	8.851703864476873E-5	0.0	0.0	1.327755579671531E-4	4.425851932238437E-5
17	8.851703864476873E-5	0.0	0.0	1.7703407728953747E-4	4.425851932238437E-5
18	8.851703864476873E-5	0.0	0.0	2.2129259661192183E-4	4.425851932238437E-5
19	1.327755579671531E-4	0.0	0.0	3.5406815457907494E-4	4.425851932238437E-5
20	1.327755579671531E-4	0.0	0.0	5.753607511909968E-4	4.425851932238437E-5
21	1.327755579671531E-4	0.0	0.0	6.196192705133811E-4	8.851703864476873E-5
22	1.327755579671531E-4	0.0	0.0	7.966533478029185E-4	8.851703864476873E-5
23	1.327755579671531E-4	0.0	0.0	0.0010179459444148405	8.851703864476873E-5
24	2.2129259661192183E-4	0.0	0.0	0.0013720140989939152	8.851703864476873E-5
25	2.2129259661192183E-4	0.0	0.0	0.001947374850184912	8.851703864476873E-5
26	2.2129259661192183E-4	0.0	0.0	0.0022571844854416025	8.851703864476873E-5
27	2.2129259661192183E-4	0.0	0.0	0.0024784770820535243	8.851703864476873E-5
28	2.655511159343062E-4	0.0	0.0	0.003275130429856443	8.851703864476873E-5
29	2.655511159343062E-4	0.0	0.0	0.004647144528850358	8.851703864476873E-5
30	2.655511159343062E-4	0.0	0.0	0.006860070494969577	8.851703864476873E-5
31	2.655511159343062E-4	0.0	0.0	0.011285922427208014	8.851703864476873E-5
32	2.655511159343062E-4	0.0	0.0	0.01633139362995983	8.851703864476873E-5
33	2.655511159343062E-4	0.0	0.0	0.02190796706458026	8.851703864476873E-5
34	2.655511159343062E-4	0.0	0.0	0.02779435013445738	8.851703864476873E-5
35	3.5406815457907494E-4	0.0	0.0	0.034079059878235965	8.851703864476873E-5
36	3.5406815457907494E-4	0.0	0.0	0.043417607455259064	8.851703864476873E-5
37	3.5406815457907494E-4	0.0	0.0	0.057713109196389216	8.851703864476873E-5
38	3.5406815457907494E-4	4.425851932238437E-5	0.0	0.07625742879246826	8.851703864476873E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2015	0.0	25.663286	1
GGTATCA	890	0.0	22.499395	1
TCAACGC	2790	0.0	18.372381	4
ATCAACG	2860	0.0	17.846182	3
CAACGCA	2875	0.0	17.829197	5
TATCAAC	2920	0.0	17.781242	2
AACGCAG	3080	0.0	16.856794	6
TAGTACC	180	5.2750693E-9	15.88856	4
ATAGTAC	220	3.0559022E-10	15.000022	3
GTATAGA	345	0.0	14.669936	1
GTAGGAC	375	0.0	14.666689	3
GTAATAC	300	0.0	14.666688	3
ACGCAGA	3635	0.0	14.101498	7
CGCAGAG	3720	0.0	13.779286	8
GTATTAC	260	2.564775E-10	13.541478	1
GTCCTAA	230	9.129508E-9	13.394288	1
TATACTG	445	0.0	13.34804	5
AGAGTAC	3900	0.0	13.256137	11
TAACGCA	100	0.0087647755	13.199728	4
TAGTACT	255	2.7921487E-9	12.940909	4
>>END_MODULE