FastQCFastQC Report
Thu 2 Feb 2017
SRR4062116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003377
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35190.1756534092185345No Hit
TATCAACGCAGAGTACTTTTTTTTT24400.1217943502396204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA601.465396E-615.8353349
GGTATCA8400.013.4522981
CGTTATT802.0080624E-613.0579482
CGCGTAA751.4779262E-512.66763310
GTATCAA19850.011.7201831
ACGGTAT1251.8291757E-811.401449
TTTGCGC600.005875508811.08362517
GCGTAAC951.3590981E-511.00111511
GGGTATA951.3674055E-510.9950681
GTCCTAG2700.010.9025161
TAGACCG700.001491709810.8571585
CGAGCCG1252.239176E-710.64054715
TAGGACG2500.010.6392164
CCTAATA1355.718175E-810.5518772
AAGACGG2750.010.363655
TAAGACT2200.010.3628724
CCTATAC1106.063019E-610.361323
AGACGGA2600.010.2318066
CGTCGTA1303.8382495E-710.2315510
TAGCACC1502.447996E-810.13258654
Produced by FastQC (version 0.11.5)