##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062116_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003377 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.252972356176596 32.0 32.0 32.0 32.0 32.0 2 30.843639015522292 32.0 32.0 32.0 32.0 32.0 3 30.864643549366892 32.0 32.0 32.0 32.0 32.0 4 30.8656942752163 32.0 32.0 32.0 32.0 32.0 5 30.800382554057474 32.0 32.0 32.0 32.0 32.0 6 34.43710295166611 36.0 36.0 36.0 32.0 36.0 7 34.39524013702863 36.0 36.0 36.0 32.0 36.0 8 34.342208680642734 36.0 36.0 36.0 32.0 36.0 9 34.45817237594322 36.0 36.0 36.0 32.0 36.0 10 34.19329562034505 36.0 36.0 36.0 32.0 36.0 11 34.43855699651139 36.0 36.0 36.0 32.0 36.0 12 34.29688421100971 36.0 36.0 36.0 32.0 36.0 13 34.36057417051309 36.0 36.0 36.0 32.0 36.0 14 34.27218291914103 36.0 36.0 36.0 32.0 36.0 15 34.23574793960398 36.0 36.0 36.0 32.0 36.0 16 34.228075394696056 36.0 36.0 36.0 32.0 36.0 17 34.18066444807942 36.0 36.0 36.0 32.0 36.0 18 34.176097159945435 36.0 36.0 36.0 32.0 36.0 19 34.18210102242364 36.0 36.0 36.0 32.0 36.0 20 34.15800920146333 36.0 36.0 36.0 32.0 36.0 21 34.141339348510044 36.0 36.0 36.0 32.0 36.0 22 34.1202594419323 36.0 36.0 36.0 32.0 36.0 23 34.08375058713362 36.0 36.0 36.0 32.0 36.0 24 34.05644069987826 36.0 36.0 36.0 32.0 36.0 25 33.71030564891181 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 36.0 5 142.0 6 447.0 7 89.0 8 377.0 9 275.0 10 144.0 11 37.0 12 73.0 13 84.0 14 309.0 15 481.0 16 717.0 17 976.0 18 1290.0 19 1799.0 20 2598.0 21 3736.0 22 5322.0 23 8088.0 24 11178.0 25 15875.0 26 22090.0 27 28424.0 28 38200.0 29 50912.0 30 67144.0 31 91061.0 32 129855.0 33 184813.0 34 410546.0 35 926254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.31113666812421 16.95258575076721 11.645861660686725 26.09041592042185 2 16.886021724361864 18.636918836382442 37.70316361456404 26.773895824691657 3 19.76998642280968 21.639096318185448 27.752525756728698 30.838391502276174 4 13.255902650202081 14.838645585392737 34.073777137014964 37.831674627390214 5 15.411345205204475 35.70104975330323 33.15493735584805 15.73266768564425 6 36.19568132879732 33.65333850136685 15.894150548612398 14.256829621223435 7 31.67728430879225 29.540197548606383 19.21534217223516 19.56717597036621 8 28.293700129523323 33.75866487039673 18.215866207205334 19.731768792874618 9 26.634789144584285 14.896311429530954 18.413616846044363 40.0552825798404 10 16.705844836453842 26.958162912876517 30.732912511639743 25.603079739029905 11 37.07549131537959 21.37814238898542 21.35154967538894 20.194816620246055 12 24.507741875504205 23.316995538512444 28.408769427164195 23.76649315881916 13 29.961439942022466 19.55537229323637 24.454373930102086 26.028813834639074 14 23.22742980507587 19.19521647547394 24.82105510398369 32.75629861546649 15 25.198332170397723 27.079788712260406 21.850926982971824 25.870952134370047 16 25.824810404927597 25.968840190407178 23.09199265358138 25.114356751083843 17 24.208162096434425 25.998021873342918 24.981220791020764 24.812595239201894 18 24.572133615046184 25.472941773791508 25.55944454327937 24.395480067882936 19 25.718614141093155 24.93096037897718 24.940503760641995 24.409921719287674 20 25.898475772593482 24.53932678582224 24.880475529772323 24.68172191181195 21 25.9366381550022 24.413295895119706 24.723759942443742 24.926306007434352 22 25.792223742902724 24.37534259033017 24.889309282930018 24.94312438383708 23 24.80379197303095 24.579332987627527 25.15374416371668 25.463130875624845 24 24.78808245473613 24.71938349806094 25.222060009413006 25.27047403778992 25 25.06963384712079 24.58490732890308 25.03675917705408 25.308699646922044 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 198.0 1 198.0 2 397.0 3 596.0 4 596.0 5 596.0 6 1269.0 7 1942.0 8 1942.0 9 1942.0 10 2433.5 11 2925.0 12 2925.0 13 2925.0 14 3983.0 15 5041.0 16 5041.0 17 5041.0 18 8379.5 19 11718.0 20 11718.0 21 11718.0 22 18045.0 23 24372.0 24 24372.0 25 24372.0 26 35728.5 27 47085.0 28 47085.0 29 47085.0 30 60813.5 31 74542.0 32 74542.0 33 74542.0 34 92660.5 35 110779.0 36 110779.0 37 110779.0 38 133827.5 39 156876.0 40 156876.0 41 156876.0 42 180725.0 43 204574.0 44 204574.0 45 204574.0 46 228503.0 47 252432.0 48 252432.0 49 252432.0 50 265920.5 51 279409.0 52 279409.0 53 279409.0 54 268985.0 55 258561.0 56 258561.0 57 258561.0 58 238152.5 59 217744.0 60 217744.0 61 217744.0 62 188578.5 63 159413.0 64 159413.0 65 159413.0 66 130696.0 67 101979.0 68 101979.0 69 101979.0 70 76650.0 71 51321.0 72 51321.0 73 51321.0 74 37312.0 75 23303.0 76 23303.0 77 23303.0 78 17377.0 79 11451.0 80 11451.0 81 11451.0 82 7767.0 83 4083.0 84 4083.0 85 4083.0 86 2873.5 87 1664.0 88 1664.0 89 1664.0 90 1104.5 91 545.0 92 545.0 93 545.0 94 352.0 95 159.0 96 159.0 97 159.0 98 412.0 99 665.0 100 665.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.489043250471579E-4 2 1.9966286924527933E-4 3 8.485671942924373E-4 4 0.010432384918065847 5 0.035739653594905 6 0.0661882411548101 7 0.107218960784715 8 0.10946516806372439 9 0.11904898578749781 10 0.13417344813282772 11 0.14121156427372383 12 0.13472252102325225 13 0.12988069644405423 14 0.11690260994311105 15 0.13442302671938433 16 0.11989755298179025 17 0.12448979897443167 18 0.11405741405636582 19 0.09933227744952648 20 0.09503952576075296 21 0.09269348704712094 22 0.10382469200754525 23 0.08360882649646073 24 0.09439062143570581 25 0.09164525698358322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2003377.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.464818426274526 #Duplication Level Percentage of deduplicated Percentage of total 1 65.13232444647599 29.61229304640233 2 16.38552927591257 14.899302266955413 3 7.15751255817708 9.762450265239018 4 3.6255424528453464 6.593385172614546 5 2.1762140271661257 4.947058780090979 6 1.2920624361810285 3.5246030433828235 7 0.8896419839081864 2.8313187883943436 8 0.633596417351904 2.30450768563539 9 0.45842776827845766 1.8758101721708098 >10 2.113951398000034 16.20208473154917 >50 0.08990002368498416 2.797630039596945 >100 0.04254954807281821 3.5574375447049578 >500 0.0023080377141625895 0.6579394127831318 >1k 4.396262312690644E-4 0.43417905048018757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3519 0.1756534092185345 No Hit TATCAACGCAGAGTACTTTTTTTTT 2440 0.1217943502396204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4974715193395951E-4 2 0.0 0.0 0.0 0.0 1.4974715193395951E-4 3 0.0 0.0 0.0 0.0 1.9966286924527935E-4 4 0.0 0.0 0.0 0.0 1.9966286924527935E-4 5 0.0 0.0 0.0 0.0 1.9966286924527935E-4 6 0.0 0.0 0.0 0.0 2.4957858655659916E-4 7 0.0 0.0 0.0 0.0 2.4957858655659916E-4 8 0.0 0.0 0.0 0.0 2.9949430386791903E-4 9 0.0 0.0 0.0 0.0 2.9949430386791903E-4 10 0.0 0.0 0.0 0.0 2.9949430386791903E-4 11 0.0 0.0 0.0 0.0 2.9949430386791903E-4 12 0.0 0.0 0.0 0.0 6.489043250471579E-4 13 0.0 0.0 0.0 0.0 8.98482911603757E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGTA 60 1.465396E-6 15.835334 9 GGTATCA 840 0.0 13.452298 1 CGTTATT 80 2.0080624E-6 13.057948 2 CGCGTAA 75 1.4779262E-5 12.667633 10 GTATCAA 1985 0.0 11.720183 1 ACGGTAT 125 1.8291757E-8 11.40144 9 TTTGCGC 60 0.0058755088 11.083625 17 GCGTAAC 95 1.3590981E-5 11.001115 11 GGGTATA 95 1.3674055E-5 10.995068 1 GTCCTAG 270 0.0 10.902516 1 TAGACCG 70 0.0014917098 10.857158 5 CGAGCCG 125 2.239176E-7 10.640547 15 TAGGACG 250 0.0 10.639216 4 CCTAATA 135 5.718175E-8 10.551877 2 AAGACGG 275 0.0 10.36365 5 TAAGACT 220 0.0 10.362872 4 CCTATAC 110 6.063019E-6 10.36132 3 AGACGGA 260 0.0 10.231806 6 CGTCGTA 130 3.8382495E-7 10.23155 10 TAGCACC 150 2.447996E-8 10.1325865 4 >>END_MODULE