FastQCFastQC Report
Thu 2 Feb 2017
SRR4062116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003377
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.026.4588051
GTATCAA22800.025.4864061
TATGCCG1557.221388E-1018.4506935
GTAGGAC6150.016.8121683
TATCAAC34150.016.7508872
TCAACGC34550.016.4275444
TAGGACC7500.016.4258464
ATCAACG34450.016.4113733
CCGTCGT2700.016.2954859
CGTCGTA2750.015.999610
CAACGCA35800.015.7925075
ACCGTCG2950.015.6602348
AACGCAG36700.015.58505256
TTACGGT850.002981847415.5286364
ATACGAA2001.2769306E-915.40038440
ATACCGT2455.456968E-1215.26454456
GTCCTAT3650.015.0760181
GATTTCG1901.0770236E-815.05300741
TGTAGGA8200.014.7572032
CGTATAT1201.18188436E-414.66630110
Produced by FastQC (version 0.11.5)