##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2003377
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284235568243023	32.0	32.0	32.0	32.0	32.0
2	31.437507768133507	32.0	32.0	32.0	32.0	32.0
3	31.565587505496968	32.0	32.0	32.0	32.0	32.0
4	31.643911755001678	32.0	32.0	32.0	32.0	32.0
5	31.61139615758791	32.0	32.0	32.0	32.0	32.0
6	35.26702263228539	36.0	36.0	36.0	36.0	36.0
7	35.274042279610875	36.0	36.0	36.0	36.0	36.0
8	35.21403510173073	36.0	36.0	36.0	36.0	36.0
9	35.30752125036875	36.0	36.0	36.0	36.0	36.0
10	35.18500861295702	36.0	36.0	36.0	36.0	36.0
11	35.30952586557598	36.0	36.0	36.0	36.0	36.0
12	35.23791478089246	36.0	36.0	36.0	36.0	36.0
13	35.26298894316946	36.0	36.0	36.0	36.0	36.0
14	35.230818263362316	36.0	36.0	36.0	36.0	36.0
15	35.206798820192105	36.0	36.0	36.0	36.0	36.0
16	35.21865979293962	36.0	36.0	36.0	36.0	36.0
17	35.2072835018072	36.0	36.0	36.0	36.0	36.0
18	35.20024987808086	36.0	36.0	36.0	36.0	36.0
19	35.18773101617918	36.0	36.0	36.0	36.0	36.0
20	35.187514381966054	36.0	36.0	36.0	36.0	36.0
21	35.17925832232276	36.0	36.0	36.0	36.0	36.0
22	35.16011913883408	36.0	36.0	36.0	36.0	36.0
23	35.131678660581606	36.0	36.0	36.0	36.0	36.0
24	35.1195241834163	36.0	36.0	36.0	36.0	36.0
25	35.10719899449779	36.0	36.0	36.0	36.0	36.0
26	35.06160497999129	36.0	36.0	36.0	36.0	36.0
27	35.046177529241874	36.0	36.0	36.0	36.0	36.0
28	35.028888721393926	36.0	36.0	36.0	36.0	36.0
29	35.009714097745956	36.0	36.0	36.0	36.0	36.0
30	34.984819632051284	36.0	36.0	36.0	36.0	36.0
31	34.985388671228634	36.0	36.0	36.0	36.0	36.0
32	34.96079569646652	36.0	36.0	36.0	36.0	36.0
33	34.94515510560419	36.0	36.0	36.0	36.0	36.0
34	34.93601753439318	36.0	36.0	36.0	36.0	36.0
35	34.91498454858971	36.0	36.0	36.0	36.0	36.0
36	34.892103183774196	36.0	36.0	36.0	32.0	36.0
37	34.881403749768516	36.0	36.0	36.0	32.0	36.0
38	34.87562700380408	36.0	36.0	36.0	32.0	36.0
39	34.84339043525008	36.0	36.0	36.0	32.0	36.0
40	34.834607764789155	36.0	36.0	36.0	32.0	36.0
41	34.807782559148876	36.0	36.0	36.0	32.0	36.0
42	34.79103783261962	36.0	36.0	36.0	32.0	36.0
43	34.769800691532346	36.0	36.0	36.0	32.0	36.0
44	34.733583843679945	36.0	36.0	36.0	32.0	36.0
45	34.71505163531377	36.0	36.0	36.0	32.0	36.0
46	34.693002864663015	36.0	36.0	36.0	32.0	36.0
47	34.664790501238656	36.0	36.0	36.0	32.0	36.0
48	34.64803629072311	36.0	36.0	36.0	32.0	36.0
49	34.622456981386925	36.0	36.0	36.0	32.0	36.0
50	34.211473427118314	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	24.0
21	46.0
22	142.0
23	393.0
24	915.0
25	2155.0
26	4571.0
27	8354.0
28	15279.0
29	24369.0
30	36494.0
31	55340.0
32	84238.0
33	143249.0
34	326064.0
35	1301742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87958243019157	17.505845767604335	12.097181298936302	26.51739050326779
2	16.33596718180543	18.945039607563398	37.4441103810303	27.274882829600873
3	19.174681075048007	22.06326237127322	27.909658270984256	30.85239828269452
4	13.072227543792305	15.192197973721372	34.09083762067749	37.64473686180884
5	15.122365885202834	35.98384128399198	32.99069521113599	15.903097619669188
6	36.348629861423774	33.845702300219536	15.664723611267792	14.1409442270889
7	31.671772212618993	29.501836149661298	19.246552196616015	19.579839441103697
8	28.398748712798437	33.78924685668249	18.121352096984243	19.69065233353483
9	26.765606273806675	14.690844509046475	18.27189790039518	40.271651316751665
10	16.6174081237911	26.954564172958133	30.853884070630812	25.574143632619954
11	37.36885269222917	21.229903308264	21.304427474209795	20.096816525297037
12	24.55364117687285	23.17696569342665	28.441376735382306	23.828016394318194
13	30.06433636804256	19.503817803638558	24.413927084118466	26.01791874420042
14	23.310939478690234	19.00166568748668	24.885530781275815	32.80186405254727
15	25.396018822218686	27.015983511840258	21.778676704384647	25.809320961556416
16	25.957818737976414	25.744925759501314	23.176383205364605	25.120872297157664
17	24.32138334422328	25.93665595641759	24.940737564622136	24.801223134736997
18	24.824608473201472	25.304398826979472	25.513146565171276	24.357846134647783
19	25.828065140076124	24.81232919448431	25.04313970175329	24.31646596368628
20	26.04924399923928	24.358793253970433	24.921894147414534	24.670068599375753
21	26.02821437413646	24.29134620760157	24.672303152278953	25.008136265983023
22	25.865825553552824	24.3104518021321	24.899956423578786	24.923766220736287
23	24.927372595059538	24.413140618635744	25.236151376476506	25.423335409828212
24	24.918361907081824	24.498715653848496	25.262884143291696	25.32003829577798
25	25.26934588284153	24.299281411540143	25.205503521063488	25.22586918455484
26	24.93332461466045	24.95738415853742	25.157048423076596	24.952242803725536
27	25.071891470709062	24.85221130227262	25.007449957995714	25.0684472690226
28	25.168854752716573	24.58503236330764	24.984413599427757	25.261699284548023
29	24.81821852910625	25.008198697492773	24.96701802515465	25.20656474824633
30	25.024234358979992	24.731575148476008	25.073302072424543	25.170888420119457
31	25.251926260030626	24.601669991075013	24.83517723189595	25.31122651699841
32	25.22509751147326	24.75049342253042	24.86295482475888	25.161454241237447
33	24.918836841085927	24.732101676573297	25.015523826827966	25.333537655512806
34	25.237638623570334	24.67093982749954	25.09238243608104	24.999039112849083
35	25.24959181299919	24.70545894894156	25.298060119668296	24.746889118390953
36	25.02328571699543	24.82806505425347	25.024234117044546	25.12441511170655
37	25.455650879554824	24.622543418675683	24.969413625125604	24.95239207664389
38	24.912335521471995	24.887477494250955	25.014463079090955	25.18572390518609
39	25.48422472879272	24.48709840532666	24.81330087448218	25.215375991398435
40	25.50122942595841	24.682011446689433	24.854071181916108	24.96268794543605
41	25.068310443386444	24.89774647915446	25.144432786217514	24.88951029124159
42	25.40969244800129	24.81930802173046	24.98773256339257	24.783266966875676
43	25.171593415205646	24.55405679280301	25.088379968471735	25.185969823519606
44	24.928966353113598	24.806616761744262	25.061399427737367	25.20301745740477
45	25.219983068242176	24.882246412983143	24.79973404361525	25.098036475159436
46	25.262425954424817	24.784371198511277	24.758464294645858	25.194738552418052
47	25.215599968652707	24.833392650258588	25.053023341251684	24.897984039837016
48	25.14214061013389	25.259197583185326	24.651549594718308	24.947112211962473
49	25.1921200216642	24.743062089896444	24.69938426749796	25.365433620941396
50	25.128185929497736	25.27943334631166	24.608853215130733	24.98352750905987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	80.0
2	82.0
3	178.0
4	274.0
5	297.0
6	320.0
7	252.0
8	184.0
9	278.0
10	372.0
11	621.0
12	870.0
13	1661.5
14	2453.0
15	3113.0
16	3773.0
17	3722.5
18	3672.0
19	3655.0
20	3638.0
21	4161.5
22	4685.0
23	5808.5
24	6932.0
25	8767.0
26	10602.0
27	13034.5
28	15467.0
29	18781.0
30	22095.0
31	26017.0
32	29939.0
33	35160.5
34	40382.0
35	46769.5
36	53157.0
37	59395.0
38	65633.0
39	73248.0
40	80863.0
41	88337.0
42	95811.0
43	102194.5
44	108578.0
45	117271.0
46	125964.0
47	132204.5
48	138445.0
49	144532.5
50	150620.0
51	154161.0
52	157702.0
53	159672.0
54	161642.0
55	159535.0
56	157428.0
57	149534.0
58	141640.0
59	131705.5
60	121771.0
61	108503.5
62	95236.0
63	82319.5
64	69403.0
65	58830.0
66	48257.0
67	40219.0
68	32181.0
69	26714.0
70	21247.0
71	16763.5
72	12280.0
73	10329.0
74	8378.0
75	6381.0
76	4384.0
77	3630.5
78	2877.0
79	2402.0
80	1927.0
81	1506.0
82	1085.0
83	791.0
84	497.0
85	402.5
86	308.0
87	218.0
88	128.0
89	91.0
90	54.0
91	38.0
92	22.0
93	17.0
94	12.0
95	11.5
96	11.0
97	10.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032245553383112614
2	0.004891740296509344
3	2.9949430386791903E-4
4	0.0
5	0.0
6	1.4974715193395951E-4
7	0.0
8	0.0
9	0.0
10	9.983143462263966E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0011480614981603562
17	0.0
18	9.983143462263966E-5
19	9.983143462263966E-5
20	2.9949430386791903E-4
21	4.991571731131983E-5
22	0.0
23	4.991571731131983E-5
24	0.0011480614981603562
25	2.4957858655659916E-4
26	6.988200423584777E-4
27	4.991571731131983E-4
28	0.0013976400847169554
29	4.991571731131983E-4
30	0.0011480614981603562
31	6.489043250471579E-4
32	0.0011480614981603562
33	2.4957858655659916E-4
34	9.983143462263967E-4
35	2.9949430386791903E-4
36	1.4974715193395951E-4
37	0.0016971343885848747
38	0.0
39	9.983143462263967E-4
40	1.4974715193395951E-4
41	0.0011480614981603562
42	0.005291066034999903
43	0.0047419931445753846
44	0.005041487448443304
45	0.0020465444097641132
46	0.002495785865565992
47	6.988200423584777E-4
48	0.003943341667594267
49	0.0035939316464150286
50	0.001846881540518834
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2003377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.02814693457146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.84843203743418	29.388250113610198
2	17.304643408708166	15.930013389327657
3	7.381245435603702	10.192351484103062
4	3.7821980121623833	6.963502633578171
5	2.15138281089183	4.951208206612026
6	1.3621445563497083	3.7618193871474617
7	0.9090260453360673	2.9288549067456646
8	0.6463511485184439	2.380027650826876
9	0.4562369365437697	1.8899766676993848
>10	2.0409353162264705	15.719960113510318
>50	0.08098398524080036	2.563816758126439
>100	0.03456748974548751	2.7309388561957393
>500	0.0017438279896921573	0.5278939961139691
>1k	1.0898924935575888E-4	0.07138583640313342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4974715193395951E-4
2	0.0	0.0	0.0	0.0	1.4974715193395951E-4
3	0.0	0.0	0.0	0.0	1.4974715193395951E-4
4	0.0	0.0	0.0	0.0	1.4974715193395951E-4
5	0.0	0.0	0.0	0.0	1.9966286924527935E-4
6	0.0	0.0	0.0	0.0	2.9949430386791903E-4
7	0.0	0.0	0.0	0.0	2.9949430386791903E-4
8	0.0	0.0	0.0	0.0	2.9949430386791903E-4
9	0.0	0.0	0.0	0.0	2.9949430386791903E-4
10	0.0	0.0	0.0	0.0	3.4941002117923884E-4
11	0.0	0.0	0.0	0.0	3.4941002117923884E-4
12	0.0	0.0	0.0	0.0	7.986514769811174E-4
13	0.0	0.0	0.0	0.0	0.0012978086500943157
14	0.0	0.0	0.0	0.0	0.0012978086500943157
15	0.0	0.0	0.0	4.991571731131984E-5	0.0016472186712735545
16	0.0	0.0	0.0	4.991571731131984E-5	0.0016971343885848744
17	0.0	0.0	0.0	4.991571731131984E-5	0.0017470501058961944
18	0.0	0.0	0.0	1.4974715193395951E-4	0.0017470501058961944
19	0.0	0.0	0.0	3.4941002117923884E-4	0.0017470501058961944
20	0.0	0.0	0.0	4.492414558018785E-4	0.0019467129751414736
21	0.0	0.0	0.0	5.490728904245182E-4	0.0019966286924527933
22	0.0	0.0	0.0	7.986514769811174E-4	0.002096460127075433
23	0.0	0.0	0.0	9.983143462263967E-4	0.002096460127075433
24	0.0	0.0	0.0	0.0013477243674056357	0.002096460127075433
25	0.0	0.0	0.0	0.0016472186712735545	0.002096460127075433
26	0.0	0.0	0.0	0.0019467129751414736	0.0022961229963207124
27	0.0	0.0	0.0	0.002196291561698073	0.0023460387136320323
28	0.0	0.0	0.0	0.0025956173001886315	0.0023460387136320323
29	0.0	0.0	0.0	0.003444184494481069	0.0023460387136320323
30	0.0	0.0	0.0	0.0054408131869338626	0.0023460387136320323
31	0.0	0.0	0.0	0.00918449198528285	0.002445870148254672
32	0.0	0.0	0.0	0.014974715193395952	0.0026455330174999514
33	0.0	0.0	0.0	0.019816539772593976	0.002745364452122591
34	0.0	0.0	0.0	0.026006088719197636	0.0028451958867452306
35	0.0	0.0	0.0	0.03289445770815977	0.0028451958867452306
36	0.0	0.0	0.0	0.04287760117042374	0.0028451958867452306
37	0.0	0.0	0.0	0.055406446215565015	0.0028451958867452306
38	0.0	0.0	0.0	0.07242770581872508	0.0031946059079244696
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	965	0.0	26.458805	1
GTATCAA	2280	0.0	25.486406	1
TATGCCG	155	7.221388E-10	18.450693	5
GTAGGAC	615	0.0	16.812168	3
TATCAAC	3415	0.0	16.750887	2
TCAACGC	3455	0.0	16.427544	4
TAGGACC	750	0.0	16.425846	4
ATCAACG	3445	0.0	16.411373	3
CCGTCGT	270	0.0	16.295485	9
CGTCGTA	275	0.0	15.9996	10
CAACGCA	3580	0.0	15.792507	5
ACCGTCG	295	0.0	15.660234	8
AACGCAG	3670	0.0	15.5850525	6
TTACGGT	85	0.0029818474	15.528636	4
ATACGAA	200	1.2769306E-9	15.400384	40
ATACCGT	245	5.456968E-12	15.2645445	6
GTCCTAT	365	0.0	15.076018	1
GATTTCG	190	1.0770236E-8	15.053007	41
TGTAGGA	820	0.0	14.757203	2
CGTATAT	120	1.18188436E-4	14.666301	10
>>END_MODULE