FastQCFastQC Report
Thu 2 Feb 2017
SRR4062115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353219
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26290.19427749684271356No Hit
GCGCAAGACGGACCAGAGCGAAAGC20210.14934759266607991No Hit
GATTAAGAGGGACGGCCGGGGGCAT19110.1412188271078074No Hit
TCGTAGTTCCGACCATAAACGATGC17220.12725212992132093No Hit
ATCAGATACCGTCGTAGTTCCGACC16950.12525688746610858No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16920.1250351938599739No Hit
GAATAGGACCGCGGTTCTATTTTGT16920.1250351938599739No Hit
TATCAACGCAGAGTACTTTTTTTTT16590.12259656419249212No Hit
GGGTAGGCACACGCTGAGCCAGTCA16380.12104470894954919No Hit
GTATCTGATCGTCTTCGAACCTCCG15450.1141722071593733No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14550.10752139897533217No Hit
GAATAACGCCGCCGCATCGCCAGTC14490.10707801176306274No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC14450.10678242028821647No Hit
GAACTACGACGGTATCTGATCGTCT14430.10663462455079334No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT14220.10508276930785039No Hit
ACCATACTCCCCCCGGAACCCAAAG14150.1045654842268694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT456.7566294E-414.77918812
GTCTTAC1152.5465852E-1114.0401421
CGTCTTA551.9588816E-413.8189915
CGTCTAT551.9638076E-413.8148991
AACCGCG1257.2759576E-1213.6818127
GCGTTAT1600.013.6530051
CGCGGTC1351.8189894E-1213.37164610
ACCGAGT500.001498710813.30077658
CGCGTAA1650.013.24368910
ATAATAC655.4503627E-513.1541283
GTATCAA16950.013.1119291
TCGCGTA1600.013.0637469
GCGTAAC1700.012.85416811
CCTAGAC905.401798E-712.6669373
CGTTATT1650.012.6655322
CGGACAT2450.012.4098075
GTGCTAG853.952724E-612.2911971
CTAGCAG701.0904788E-412.2145483
GTGTAAG1251.4151738E-912.1571111
ACGACAG550.003065045512.0925115
Produced by FastQC (version 0.11.5)