##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062115_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1353219
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28880543356249	32.0	32.0	32.0	32.0	32.0
2	30.84573080927773	32.0	32.0	32.0	32.0	32.0
3	30.871539639925246	32.0	32.0	32.0	32.0	32.0
4	30.884272981682937	32.0	32.0	32.0	32.0	32.0
5	30.808846166067724	32.0	32.0	32.0	32.0	32.0
6	34.47747851604212	36.0	36.0	36.0	32.0	36.0
7	34.40216772008078	36.0	36.0	36.0	32.0	36.0
8	34.360378475324396	36.0	36.0	36.0	32.0	36.0
9	34.49368062375713	36.0	36.0	36.0	32.0	36.0
10	34.19032174393058	36.0	36.0	36.0	32.0	36.0
11	34.47894464975736	36.0	36.0	36.0	32.0	36.0
12	34.29585676819495	36.0	36.0	36.0	32.0	36.0
13	34.394346369656354	36.0	36.0	36.0	32.0	36.0
14	34.29890283834324	36.0	36.0	36.0	32.0	36.0
15	34.24278479684367	36.0	36.0	36.0	32.0	36.0
16	34.250467958253616	36.0	36.0	36.0	32.0	36.0
17	34.17518007063158	36.0	36.0	36.0	32.0	36.0
18	34.19062029132018	36.0	36.0	36.0	32.0	36.0
19	34.18080665435528	36.0	36.0	36.0	32.0	36.0
20	34.17294613806043	36.0	36.0	36.0	32.0	36.0
21	34.14416439615465	36.0	36.0	36.0	32.0	36.0
22	34.11534866122926	36.0	36.0	36.0	32.0	36.0
23	34.08669919650848	36.0	36.0	36.0	32.0	36.0
24	34.066604888048424	36.0	36.0	36.0	32.0	36.0
25	33.715638045283136	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	26.0
5	94.0
6	261.0
7	73.0
8	209.0
9	183.0
10	90.0
11	21.0
12	52.0
13	69.0
14	195.0
15	255.0
16	420.0
17	568.0
18	854.0
19	1100.0
20	1739.0
21	2393.0
22	3539.0
23	5316.0
24	7610.0
25	10582.0
26	14857.0
27	18884.0
28	25294.0
29	33858.0
30	45079.0
31	61373.0
32	88011.0
33	126464.0
34	280960.0
35	622787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14312317793682	17.442811695197697	11.571417486633585	25.842647640231892
2	16.702630482141746	19.536185017573004	37.419487855561435	26.34169664472381
3	19.04155233966076	23.199446648669937	28.26281823428606	29.49618277738324
4	12.577064349008566	15.522322979254891	35.639831792415876	36.26078087932066
5	14.971192612535095	36.3837229759798	33.3045037692807	15.340580642204413
6	34.70830551223722	35.41808545013979	16.425427261790414	13.448181775832582
7	30.48052546584494	30.184769687030023	20.443101041507155	18.89160380561788
8	28.046468309361643	33.21844972973133	19.13200370172281	19.603078259184215
9	27.116973817573815	14.062086160083453	18.640570553463494	40.18036946887924
10	16.30479742203773	26.60554735451791	30.91645670777051	26.173198515673846
11	37.52736120340456	21.15355869542374	21.942686141282227	19.376393959889473
12	24.40811457966442	23.650924492616788	28.77714503893613	23.163815888782665
13	29.263840630752746	19.430748012619787	25.060450097963194	26.24496125866427
14	23.469699536650342	19.833703120341443	24.809810613318696	31.88678672968952
15	25.234748558210985	27.401760780674923	21.86065887945208	25.50283178166201
16	25.82627950971826	25.836859320332252	23.655198793161745	24.681662376787745
17	23.906825257774674	26.120476365229127	25.109428456429868	24.863269920566335
18	24.77856269377845	25.14475818890805	26.149391486657535	23.927287630655965
19	25.561320911723477	25.051684794869615	25.06788367869995	24.31911061470696
20	25.749608914168213	24.511447812693	24.53060454658082	25.208338726557965
21	26.870765154837883	24.33657020163059	24.215344811900945	24.577319831630582
22	26.022038922064887	24.394306895355435	24.986074957781046	24.597579224798633
23	24.273722978619553	24.53604206571659	25.50315418919219	25.68708076647167
24	24.691257580341073	25.08962535234394	25.296649376450148	24.92246769086483
25	24.858603706516988	24.639898938723466	25.240246114976433	25.261251239783117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	868.0
1	868.0
2	666.0
3	464.0
4	464.0
5	464.0
6	1037.0
7	1610.0
8	1610.0
9	1610.0
10	1963.0
11	2316.0
12	2316.0
13	2316.0
14	3073.0
15	3830.0
16	3830.0
17	3830.0
18	6277.5
19	8725.0
20	8725.0
21	8725.0
22	13966.0
23	19207.0
24	19207.0
25	19207.0
26	29263.0
27	39319.0
28	39319.0
29	39319.0
30	49590.0
31	59861.0
32	59861.0
33	59861.0
34	72632.5
35	85404.0
36	85404.0
37	85404.0
38	97496.0
39	109588.0
40	109588.0
41	109588.0
42	124239.0
43	138890.0
44	138890.0
45	138890.0
46	154439.5
47	169989.0
48	169989.0
49	169989.0
50	174549.0
51	179109.0
52	179109.0
53	179109.0
54	167081.0
55	155053.0
56	155053.0
57	155053.0
58	144990.5
59	134928.0
60	134928.0
61	134928.0
62	119768.5
63	104609.0
64	104609.0
65	104609.0
66	85915.5
67	67222.0
68	67222.0
69	67222.0
70	51139.0
71	35056.0
72	35056.0
73	35056.0
74	26597.5
75	18139.0
76	18139.0
77	18139.0
78	14734.5
79	11330.0
80	11330.0
81	11330.0
82	7790.5
83	4251.0
84	4251.0
85	4251.0
86	3165.0
87	2079.0
88	2079.0
89	2079.0
90	1386.5
91	694.0
92	694.0
93	694.0
94	427.5
95	161.0
96	161.0
97	161.0
98	339.0
99	517.0
100	517.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010345701619619589
2	5.172850809809794E-4
3	7.389786871156848E-4
4	0.009532825063792334
5	0.032588960101801706
6	0.06118743529317871
7	0.10552615652011979
8	0.10463938209558099
9	0.11446779863421959
10	0.13035584040720682
11	0.1369327507225364
12	0.1311687169630341
13	0.12496129599126231
14	0.11380271781581547
15	0.13102092122561093
16	0.11734981551397075
17	0.12200538124279958
18	0.1095166414305445
19	0.09414588473853826
20	0.08971201261584415
21	0.08808625950418965
22	0.09887534833607865
23	0.07751886427843534
24	0.08882523819130532
25	0.086312710655112
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1353219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.89807866614564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7430981107989	33.177153802871054
2	15.949268419858113	14.959800900439562
3	5.834562227038688	8.20889274918547
4	2.7535701501117162	5.16548598050759
5	1.436657927507073	3.3688248250284243
6	0.8223495125410183	2.3139967278129117
7	0.5350827669750177	1.7566047588945275
8	0.35686803858382765	1.3389140277549958
9	0.24513466005074777	1.0346710113772952
>10	1.1238606256870591	9.26105803901724
>50	0.08853135354423816	2.9073596911320934
>100	0.09411902425290848	9.398187405536957
>500	0.011685902296733613	3.7341953756220585
>1k	0.005211280753948774	3.3748547048198483
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2629	0.19427749684271356	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2021	0.14934759266607991	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1911	0.1412188271078074	No Hit
TCGTAGTTCCGACCATAAACGATGC	1722	0.12725212992132093	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1695	0.12525688746610858	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1692	0.1250351938599739	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1692	0.1250351938599739	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1659	0.12259656419249212	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1638	0.12104470894954919	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1545	0.1141722071593733	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1455	0.10752139897533217	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1449	0.10707801176306274	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1445	0.10678242028821647	No Hit
GAACTACGACGGTATCTGATCGTCT	1443	0.10663462455079334	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1422	0.10508276930785039	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1415	0.1045654842268694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4779573742313698E-4
2	0.0	0.0	0.0	0.0	1.4779573742313698E-4
3	0.0	0.0	0.0	0.0	1.4779573742313698E-4
4	0.0	0.0	0.0	7.389786871156849E-5	1.4779573742313698E-4
5	0.0	0.0	0.0	7.389786871156849E-5	1.4779573742313698E-4
6	0.0	0.0	0.0	7.389786871156849E-5	2.2169360613470546E-4
7	0.0	0.0	0.0	1.4779573742313698E-4	2.2169360613470546E-4
8	0.0	0.0	0.0	2.2169360613470546E-4	2.2169360613470546E-4
9	0.0	0.0	0.0	2.2169360613470546E-4	2.2169360613470546E-4
10	0.0	0.0	0.0	3.6948934355784247E-4	2.2169360613470546E-4
11	0.0	0.0	0.0	3.6948934355784247E-4	2.2169360613470546E-4
12	0.0	0.0	0.0	3.6948934355784247E-4	3.6948934355784247E-4
13	0.0	0.0	0.0	4.433872122694109E-4	4.433872122694109E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTTT	45	6.7566294E-4	14.779188	12
GTCTTAC	115	2.5465852E-11	14.040142	1
CGTCTTA	55	1.9588816E-4	13.81899	15
CGTCTAT	55	1.9638076E-4	13.814899	1
AACCGCG	125	7.2759576E-12	13.681812	7
GCGTTAT	160	0.0	13.653005	1
CGCGGTC	135	1.8189894E-12	13.371646	10
ACCGAGT	50	0.0014987108	13.3007765	8
CGCGTAA	165	0.0	13.243689	10
ATAATAC	65	5.4503627E-5	13.154128	3
GTATCAA	1695	0.0	13.111929	1
TCGCGTA	160	0.0	13.063746	9
GCGTAAC	170	0.0	12.854168	11
CCTAGAC	90	5.401798E-7	12.666937	3
CGTTATT	165	0.0	12.665532	2
CGGACAT	245	0.0	12.409807	5
GTGCTAG	85	3.952724E-6	12.291197	1
CTAGCAG	70	1.0904788E-4	12.214548	3
GTGTAAG	125	1.4151738E-9	12.157111	1
ACGACAG	55	0.0030650455	12.092511	5
>>END_MODULE