FastQCFastQC Report
Thu 2 Feb 2017
SRR4062115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353219
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA20180.14912589905994522No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20060.1482391246354064No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19110.1412188271078074No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT18200.13449412105505465No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG17020.12577417254708959No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16350.12082301534341447No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15620.11542847092746997No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14510.10722580750048588No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13920.10286583324650334No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC13550.10013161210417529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19400.024.8410191
GGTATCA8850.022.6269041
TACGAAT4350.021.24177241
ATACGAA4450.020.7644340
CGCAATA4600.020.08658636
GATATAC3450.019.7728651
CAATACG4650.019.39820738
CGAATGC4700.019.19255343
AATACGA4950.018.2225639
GCAATAC5100.018.11798137
TAGCGGC5050.017.86237530
GTCCTAA2100.017.8138031
GCGCAAT5200.017.76890235
TTAGACC750.001291458417.5990244
AACGCAG27950.017.2369696
CTAGCGG5250.017.18190429
TCTAGCG5350.016.86074628
ATACACT4450.016.8080584
TCAACGC28800.016.728244
ATCAACG29050.016.508553
Produced by FastQC (version 0.11.5)