##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1353219
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275694473695683	32.0	32.0	32.0	32.0	32.0
2	31.46446805727676	32.0	32.0	32.0	32.0	32.0
3	31.590455055685737	32.0	32.0	32.0	32.0	32.0
4	31.665442178982115	32.0	32.0	32.0	32.0	32.0
5	31.639583097783877	32.0	32.0	32.0	32.0	32.0
6	35.29242938504411	36.0	36.0	36.0	36.0	36.0
7	35.29203107553175	36.0	36.0	36.0	36.0	36.0
8	35.24670138388539	36.0	36.0	36.0	36.0	36.0
9	35.32619627717317	36.0	36.0	36.0	36.0	36.0
10	35.21007094934375	36.0	36.0	36.0	36.0	36.0
11	35.331527269422025	36.0	36.0	36.0	36.0	36.0
12	35.24803302347957	36.0	36.0	36.0	36.0	36.0
13	35.28617762535111	36.0	36.0	36.0	36.0	36.0
14	35.248647114768566	36.0	36.0	36.0	36.0	36.0
15	35.229203107553175	36.0	36.0	36.0	36.0	36.0
16	35.23399464536043	36.0	36.0	36.0	36.0	36.0
17	35.2125347042866	36.0	36.0	36.0	36.0	36.0
18	35.20702044532334	36.0	36.0	36.0	36.0	36.0
19	35.19813644354683	36.0	36.0	36.0	36.0	36.0
20	35.193718089976564	36.0	36.0	36.0	36.0	36.0
21	35.185825058619486	36.0	36.0	36.0	36.0	36.0
22	35.17372428261797	36.0	36.0	36.0	36.0	36.0
23	35.14072666730219	36.0	36.0	36.0	36.0	36.0
24	35.122054153836146	36.0	36.0	36.0	36.0	36.0
25	35.105712379149274	36.0	36.0	36.0	36.0	36.0
26	35.06719976589155	36.0	36.0	36.0	36.0	36.0
27	35.0542750286539	36.0	36.0	36.0	36.0	36.0
28	35.026210835053305	36.0	36.0	36.0	36.0	36.0
29	35.00379613351571	36.0	36.0	36.0	36.0	36.0
30	34.99027725741362	36.0	36.0	36.0	36.0	36.0
31	34.99180546533857	36.0	36.0	36.0	36.0	36.0
32	34.96463986982152	36.0	36.0	36.0	36.0	36.0
33	34.93746762349627	36.0	36.0	36.0	36.0	36.0
34	34.932511293441785	36.0	36.0	36.0	36.0	36.0
35	34.91531304245654	36.0	36.0	36.0	36.0	36.0
36	34.898184994446574	36.0	36.0	36.0	32.0	36.0
37	34.879465925323245	36.0	36.0	36.0	32.0	36.0
38	34.855250332725156	36.0	36.0	36.0	32.0	36.0
39	34.8385863633307	36.0	36.0	36.0	32.0	36.0
40	34.82568527341103	36.0	36.0	36.0	32.0	36.0
41	34.7933786031677	36.0	36.0	36.0	32.0	36.0
42	34.75399621199525	36.0	36.0	36.0	32.0	36.0
43	34.76638297274868	36.0	36.0	36.0	32.0	36.0
44	34.698288303667034	36.0	36.0	36.0	32.0	36.0
45	34.69439831985806	36.0	36.0	36.0	32.0	36.0
46	34.65031750219292	36.0	36.0	36.0	32.0	36.0
47	34.63883303441646	36.0	36.0	36.0	32.0	36.0
48	34.60326894612032	36.0	36.0	36.0	32.0	36.0
49	34.590570336360926	36.0	36.0	36.0	32.0	36.0
50	34.18440621954022	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	34.0
22	88.0
23	251.0
24	636.0
25	1547.0
26	3138.0
27	5981.0
28	10516.0
29	16846.0
30	24832.0
31	36822.0
32	55997.0
33	95127.0
34	217595.0
35	883798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78482147675217	17.998097269870556	12.019207668874692	26.197873584502577
2	16.064587360990405	20.003044760941926	37.16596311996358	26.76640475810409
3	18.4026461466155	23.662793662195085	28.617593830387385	29.316966360802034
4	12.321961428239945	16.027424997302724	35.7026731834782	35.947940390979134
5	14.572290220577749	36.735517310945234	33.25322804364999	15.438964424827024
6	34.8144940430884	35.60712316862472	16.254439417743484	13.3239433705434
7	30.442374811468063	30.268123637046184	20.43771185595236	18.85178969553339
8	28.041654750635338	33.313307010912496	19.078212765265636	19.566825473186526
9	27.196706519787263	13.832129167562679	18.529594987950954	40.4415693246991
10	16.238908853629752	26.648679925422268	30.988036674034287	26.124374546913693
11	37.74688354213176	20.956918281519844	22.02865907144372	19.267539104904678
12	24.434478085217545	23.52442583203458	28.90818115914719	23.13291492360069
13	29.493378381474102	19.252463939687516	25.161337521864528	26.09282015697385
14	23.56085748130938	19.69511217326981	24.933658188364188	31.810372157056616
15	25.22703272714912	27.38736302106311	21.927640684915005	25.457963566872767
16	25.834790358361648	25.72519941413553	23.69935575600016	24.74065447150266
17	23.99796337473831	26.110703441202055	25.07591158563396	24.81542159842568
18	24.839345677260688	24.983225146613698	26.260478741013998	23.916950435111616
19	25.708403443936273	24.9049858153041	25.161633113339377	24.224977627420248
20	25.785648094093027	24.239477274104466	24.86177352584292	25.11310110595959
21	26.950404923371607	24.241752443617774	24.339741017529313	24.46810161548131
22	25.972810018186266	24.30604358939684	25.19274411606695	24.528402276349947
23	24.354890080615185	24.228968112330673	25.767300045299397	25.64884176175475
24	24.854660077874264	24.943338306704	25.455381179671697	24.746620435750035
25	25.038888868361642	24.36242577860872	25.379559050113986	25.21912630291565
26	24.438391352124686	25.617808309273276	25.785631361258517	24.158168977343518
27	25.104769167898922	24.672982006528166	25.31996071571881	24.902288109854105
28	24.672016482497323	24.694186269068787	25.699512190796135	24.93428505763775
29	24.62365551667695	24.714180673433265	25.59497197415045	25.067191835739333
30	24.440788914581056	25.029023773999576	25.8831467017834	24.64704060963597
31	25.211201201289967	24.682680910308218	24.89669024513528	25.20942764326654
32	25.119106122132273	24.69300660284288	24.730621007164473	25.457266267860373
33	24.50641693091352	24.462152042133674	25.523327005203157	25.50810402174965
34	25.274735019542376	24.78309031911458	25.52554549801103	24.41662916333201
35	25.74914019766216	24.412031173099972	25.76162894670334	24.077199682534523
36	24.254554698503863	25.25387631556778	25.17074115183215	25.320827834096203
37	25.45373794342908	25.005542401589114	25.049733816926345	24.490985838055458
38	24.668586533295795	24.651590023492133	25.360787869517054	25.31903557369502
39	25.549784106419317	24.425940336074216	24.765280853790202	25.258994703716265
40	25.826511323378792	24.643775060633246	25.365314383935182	24.16439923205278
41	24.501922843857983	25.225243200581733	25.7535449200564	24.519289035503885
42	25.86897867154805	25.474792875095424	25.032645589723085	23.62358286363344
43	25.21493846338028	24.406840785173863	25.370498190186364	25.00772256125949
44	24.63143705737898	25.231956877267375	24.862673399543738	25.273932665809905
45	24.88774656588737	25.376370467370386	24.97649993940236	24.759383027339883
46	24.643511676027195	25.000886786875554	24.996600650310373	25.359000886786877
47	24.899812076053404	24.81985387305859	25.50969659482991	24.77063745605809
48	25.85108065405325	25.327492199751394	24.18550033181541	24.63592681437995
49	24.79099063014302	25.685187881188526	24.528347900373124	24.99547358829533
50	25.01332032707777	25.714919135822996	24.511249302576495	24.760511234522742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	420.0
1	236.0
2	52.0
3	179.0
4	306.0
5	307.5
6	309.0
7	227.5
8	146.0
9	229.0
10	312.0
11	502.5
12	693.0
13	1296.0
14	1899.0
15	2440.0
16	2981.0
17	3058.0
18	3135.0
19	3118.5
20	3102.0
21	3636.5
22	4171.0
23	4723.5
24	5276.0
25	6915.5
26	8555.0
27	11109.5
28	13664.0
29	16035.5
30	18407.0
31	20888.5
32	23370.0
33	26699.5
34	30029.0
35	34251.5
36	38474.0
37	45208.5
38	51943.0
39	55875.5
40	59808.0
41	63953.0
42	68098.0
43	70013.5
44	71929.0
45	77890.0
46	83851.0
47	89469.5
48	95088.0
49	99848.5
50	104609.0
51	100379.5
52	96150.0
53	96110.0
54	96070.0
55	97552.0
56	99034.0
57	96372.0
58	93710.0
59	86647.5
60	79585.0
61	69385.0
62	59185.0
63	51052.0
64	42919.0
65	36169.0
66	29419.0
67	25703.5
68	21988.0
69	19675.0
70	17362.0
71	13431.5
72	9501.0
73	8393.5
74	7286.0
75	5221.5
76	3157.0
77	2872.5
78	2588.0
79	2284.5
80	1981.0
81	1554.5
82	1128.0
83	958.0
84	788.0
85	623.0
86	458.0
87	301.0
88	144.0
89	95.5
90	47.0
91	37.5
92	28.0
93	26.0
94	24.0
95	21.5
96	19.0
97	16.5
98	14.0
99	10.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03148049207112818
2	0.005542340153367637
3	4.433872122694109E-4
4	7.389786871156849E-5
5	0.0
6	7.389786871156849E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.694893435578424E-4
17	0.0
18	2.2169360613470546E-4
19	0.0
20	3.694893435578424E-4
21	0.0
22	0.0
23	0.0
24	8.867744245388219E-4
25	2.9559147484627395E-4
26	5.911829496925479E-4
27	4.433872122694109E-4
28	0.0019213445865007808
29	2.9559147484627395E-4
30	0.0013301616368082327
31	5.172850809809794E-4
32	0.0010345701619619589
33	1.4779573742313698E-4
34	4.433872122694109E-4
35	7.389786871156849E-5
36	7.389786871156849E-5
37	0.0011084680306735273
38	0.0
39	4.433872122694109E-4
40	7.389786871156849E-5
41	0.0011084680306735273
42	0.0036948934355784243
43	0.003029812617174308
44	0.004286076385270972
45	0.0022908339300586234
46	0.0014040595055198014
47	8.867744245388219E-4
48	0.004212178516559404
49	0.0036948934355784243
50	0.0017735488490776437
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1353219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.25034127229113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05419210297957	33.801386765234064
2	16.41560950488713	15.8411752160694
3	6.192726870031525	8.964035546553252
4	2.7324665170134965	5.273697678440396
5	1.4352354184230915	3.4625299372496867
6	0.8289957173960619	2.3999595766576673
7	0.5224900685475162	1.7647226883160312
8	0.3519780283594758	1.3586447990954298
9	0.24357128696849661	1.0577157948325007
>10	1.0460461880067575	8.800839544033343
>50	0.08079034603819399	2.7315056319468667
>100	0.08129812946598604	8.358154313755945
>500	0.010296716485773473	3.369552456156298
>1k	0.0043031053970396605	2.8160800516592444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2018	0.14912589905994522	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2006	0.1482391246354064	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1911	0.1412188271078074	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1820	0.13449412105505465	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1702	0.12577417254708959	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1635	0.12082301534341447	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1562	0.11542847092746997	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1451	0.10722580750048588	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1392	0.10286583324650334	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1355	0.10013161210417529	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.389786871156849E-5
2	0.0	0.0	0.0	0.0	7.389786871156849E-5
3	0.0	0.0	0.0	0.0	7.389786871156849E-5
4	0.0	0.0	0.0	0.0	7.389786871156849E-5
5	0.0	0.0	0.0	0.0	2.2169360613470546E-4
6	0.0	0.0	0.0	0.0	2.2169360613470546E-4
7	0.0	0.0	0.0	0.0	2.2169360613470546E-4
8	0.0	0.0	0.0	0.0	2.2169360613470546E-4
9	0.0	0.0	0.0	0.0	2.2169360613470546E-4
10	0.0	0.0	0.0	7.389786871156849E-5	2.2169360613470546E-4
11	0.0	0.0	0.0	7.389786871156849E-5	2.9559147484627395E-4
12	0.0	0.0	0.0	7.389786871156849E-5	2.9559147484627395E-4
13	0.0	0.0	0.0	1.4779573742313698E-4	3.6948934355784247E-4
14	0.0	0.0	0.0	1.4779573742313698E-4	4.433872122694109E-4
15	0.0	0.0	0.0	1.4779573742313698E-4	5.172850809809794E-4
16	0.0	0.0	0.0	2.2169360613470546E-4	5.172850809809794E-4
17	0.0	0.0	0.0	2.2169360613470546E-4	5.172850809809794E-4
18	0.0	0.0	0.0	2.2169360613470546E-4	5.172850809809794E-4
19	0.0	0.0	0.0	2.9559147484627395E-4	5.172850809809794E-4
20	0.0	0.0	0.0	3.6948934355784247E-4	5.911829496925479E-4
21	0.0	0.0	0.0	5.172850809809794E-4	6.650808184041164E-4
22	0.0	0.0	0.0	8.128765558272534E-4	6.650808184041164E-4
23	0.0	0.0	0.0	0.0011084680306735273	6.650808184041164E-4
24	0.0	0.0	0.0	0.0015518552429429383	6.650808184041164E-4
25	0.0	0.0	0.0	0.0019213445865007806	6.650808184041164E-4
26	0.0	0.0	0.0	0.0022169360613470547	8.867744245388219E-4
27	0.0	0.0	0.0	0.002882016879751171	8.867744245388219E-4
28	0.0	0.0	0.0	0.003768791304289993	8.867744245388219E-4
29	0.0	0.0	0.0	0.0059118294969254795	8.867744245388219E-4
30	0.0	0.0	0.0	0.009015539982811356	8.867744245388219E-4
31	0.0	0.0	0.0	0.01566634816685252	8.867744245388219E-4
32	0.0	0.0	0.0	0.023277828644144075	8.867744245388219E-4
33	0.0	0.0	0.0	0.03029812617174308	8.867744245388219E-4
34	0.0	0.0	0.0	0.03650554714351483	0.0010345701619619589
35	0.0	0.0	0.0	0.044929904176633645	0.0011084680306735273
36	0.0	0.0	0.0	0.056679665301773034	0.0011084680306735273
37	0.0	0.0	0.0	0.07367617510543378	0.0011084680306735273
38	0.0	0.0	0.0	0.09702790161828943	0.0013301616368082327
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1940	0.0	24.841019	1
GGTATCA	885	0.0	22.626904	1
TACGAAT	435	0.0	21.241772	41
ATACGAA	445	0.0	20.76443	40
CGCAATA	460	0.0	20.086586	36
GATATAC	345	0.0	19.772865	1
CAATACG	465	0.0	19.398207	38
CGAATGC	470	0.0	19.192553	43
AATACGA	495	0.0	18.22256	39
GCAATAC	510	0.0	18.117981	37
TAGCGGC	505	0.0	17.862375	30
GTCCTAA	210	0.0	17.813803	1
GCGCAAT	520	0.0	17.768902	35
TTAGACC	75	0.0012914584	17.599024	4
AACGCAG	2795	0.0	17.236969	6
CTAGCGG	525	0.0	17.181904	29
TCTAGCG	535	0.0	16.860746	28
ATACACT	445	0.0	16.808058	4
TCAACGC	2880	0.0	16.72824	4
ATCAACG	2905	0.0	16.50855	3
>>END_MODULE