FastQCFastQC Report
Thu 2 Feb 2017
SRR4062114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070754
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20110.1878115795037889No Hit
GCGCAAGACGGACCAGAGCGAAAGC19410.18127413019236913No Hit
GAATAGGACCGCGGTTCTATTTTGT16340.15260274535514226No Hit
GATTAAGAGGGACGGCCGGGGGCAT16120.15054811842869603No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15930.14877366790131066No Hit
TCGTAGTTCCGACCATAAACGATGC15030.1403683759294852No Hit
GTATCTGATCGTCTTCGAACCTCCG15000.14008819953042437No Hit
GAATAACGCCGCCGCATCGCCAGTC14990.13999480739740408No Hit
GAACTACGACGGTATCTGATCGTCT14630.1366326906086739No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14420.13467145581524795No Hit
ATCAGATACCGTCGTAGTTCCGACC14210.132710221021822No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT13300.12421153691697627No Hit
TATCAACGCAGAGTACTTTTTTTTT12560.11730051907347533No Hit
ACCATACTCCCCCCGGAACCCAAAG12470.11645998987629279No Hit
GGGTAGGCACACGCTGAGCCAGTCA12330.11515250001400883No Hit
CTATTGGAGCTGGAATTACCGCGGC11610.10842826643654845No Hit
GTGCATGGCCGTTCTTAGTTGGTGG11570.10805469790446731No Hit
GTCCTATTCCATTATTCCTAGCTGC11360.10609346311104138No Hit
GCTTTGAACACTCTAATTTTTTCAA10900.10179742499210837No Hit
GAATAATGGAATAGGACCGCGGTTC10820.1010502879279461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC350.002171887816.283153
GGTATCA4900.014.3413271
CCGTCCG400.00527664314.2504229
CGTCAAT2600.013.88502619
TCGCGTA1351.8189894E-1213.3707669
CGTCTTA500.001498152213.30101515
TCTCGTC655.445483E-513.15485112
CCGTCAA2750.013.1276618
GTATCAA12850.013.0804841
CGCCAGT2900.012.7762418
GCGAATC604.0875393E-412.66763315
TAAGCCT604.0907657E-412.6664494
CGCATCG2950.012.5602813
CGCGTAA1457.2759576E-1212.44922510
ATCGCCA3000.012.35036516
TCCGTCA2950.012.23707817
CTATACC701.0904618E-412.2140754
GCATAAA701.0942762E-412.2095091
CTATTCC2350.012.1274514
GGCACCG550.00306669112.0912675
Produced by FastQC (version 0.11.5)