##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062114_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070754
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11506751317296	32.0	32.0	32.0	32.0	32.0
2	30.729913686990663	32.0	32.0	32.0	32.0	32.0
3	30.74142146562142	32.0	32.0	32.0	32.0	32.0
4	30.698028678856208	32.0	32.0	32.0	32.0	32.0
5	30.698763674943077	32.0	32.0	32.0	32.0	32.0
6	34.05722509558685	36.0	36.0	36.0	32.0	36.0
7	34.058574611908995	36.0	36.0	36.0	32.0	36.0
8	34.024145602071066	36.0	36.0	36.0	32.0	36.0
9	34.181225566283196	36.0	36.0	36.0	32.0	36.0
10	33.8955250225542	36.0	36.0	36.0	32.0	36.0
11	34.12166286560685	36.0	36.0	36.0	32.0	36.0
12	33.994200348539444	36.0	36.0	36.0	32.0	36.0
13	34.09124784964614	36.0	36.0	36.0	32.0	36.0
14	33.96539914863732	36.0	36.0	36.0	32.0	36.0
15	33.901778559781235	36.0	36.0	36.0	32.0	36.0
16	33.928520463150264	36.0	36.0	36.0	32.0	36.0
17	33.84782685845675	36.0	36.0	36.0	32.0	36.0
18	33.85493586762225	36.0	36.0	36.0	32.0	36.0
19	33.83470339592474	36.0	36.0	36.0	32.0	36.0
20	33.82569852645892	36.0	36.0	36.0	32.0	36.0
21	33.78970426447158	36.0	36.0	36.0	32.0	36.0
22	33.75559278788592	36.0	36.0	36.0	32.0	36.0
23	33.75342328863586	36.0	36.0	36.0	32.0	36.0
24	33.715785325107355	36.0	36.0	36.0	32.0	36.0
25	33.133148230125684	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	83.0
6	200.0
7	42.0
8	189.0
9	173.0
10	91.0
11	21.0
12	39.0
13	56.0
14	322.0
15	515.0
16	819.0
17	1036.0
18	1303.0
19	1505.0
20	1891.0
21	2324.0
22	3048.0
23	4186.0
24	5722.0
25	8037.0
26	11293.0
27	15426.0
28	21741.0
29	29855.0
30	42026.0
31	60882.0
32	93167.0
33	140203.0
34	282667.0
35	341875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75276559778472	17.50173944309803	11.67103278558387	26.07446217353338
2	16.953708191727117	19.324660915563	36.72413100711557	26.997499885594316
3	18.627054519780078	23.00604530099958	28.32101641000832	30.04588376921202
4	12.57535198507064	15.166974581606176	35.40726031670717	36.85041311661601
5	15.17925290658296	36.076007903476686	32.92937785812045	15.815361331819902
6	34.854461199465106	35.082471519735506	16.330117827136405	13.732949453662977
7	30.91908932484686	29.98259201364218	20.19915409527086	18.8991645662401
8	28.698285428998027	31.938571256276898	19.223221456305506	20.139921858419573
9	27.105045151855723	14.033634345704824	18.715813528165445	40.14550697427401
10	16.449225825818527	25.909141492874532	30.289161561459338	27.352471119847603
11	37.5495897216242	21.06438574210165	21.87287823326855	19.51314630300559
12	24.71232605136003	23.48326511451317	28.004971230267273	23.79943760385953
13	28.84414338427127	18.983903213559678	25.371000349726856	26.800953052442196
14	23.860245888993713	19.54741764196027	24.46772204389499	32.12461442515103
15	25.486613112883767	26.797433907212927	21.723696146184995	25.992256833718308
16	26.148001522222298	25.57109049274934	23.09308464135676	25.187823343671607
17	24.053557702917267	25.890337433352094	24.81882804420315	25.237276819527484
18	25.167198204086063	24.467831467576218	25.74894417127379	24.61602615706393
19	25.555863018001514	24.74198083927276	25.064691760588133	24.637464382137594
20	25.901776958894096	23.778475137625744	24.336082576380065	25.9836653271001
21	27.12597195969885	23.84883888324892	24.04215580147063	24.983033355581604
22	26.140765013018214	23.722695635508646	24.90064553524267	25.235893816230465
23	24.351180428203158	23.735682786442414	25.48272400468844	26.43041278066599
24	24.689975452300246	24.6353834619922	25.2071036892594	25.467537396448154
25	24.82319025905922	24.040796639352997	25.05968438783541	26.076328713752368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	241.0
3	334.0
4	334.0
5	334.0
6	784.0
7	1234.0
8	1234.0
9	1234.0
10	1424.0
11	1614.0
12	1614.0
13	1614.0
14	2215.5
15	2817.0
16	2817.0
17	2817.0
18	4782.0
19	6747.0
20	6747.0
21	6747.0
22	10540.5
23	14334.0
24	14334.0
25	14334.0
26	21177.5
27	28021.0
28	28021.0
29	28021.0
30	33945.0
31	39869.0
32	39869.0
33	39869.0
34	51123.0
35	62377.0
36	62377.0
37	62377.0
38	72825.0
39	83273.0
40	83273.0
41	83273.0
42	96206.5
43	109140.0
44	109140.0
45	109140.0
46	122728.5
47	136317.0
48	136317.0
49	136317.0
50	141698.0
51	147079.0
52	147079.0
53	147079.0
54	136175.5
55	125272.0
56	125272.0
57	125272.0
58	116944.5
59	108617.0
60	108617.0
61	108617.0
62	97332.0
63	86047.0
64	86047.0
65	86047.0
66	70832.0
67	55617.0
68	55617.0
69	55617.0
70	42108.0
71	28599.0
72	28599.0
73	28599.0
74	22009.5
75	15420.0
76	15420.0
77	15420.0
78	13066.0
79	10712.0
80	10712.0
81	10712.0
82	7432.0
83	4152.0
84	4152.0
85	4152.0
86	3016.0
87	1880.0
88	1880.0
89	1880.0
90	1262.5
91	645.0
92	645.0
93	645.0
94	364.5
95	84.0
96	84.0
97	84.0
98	244.5
99	405.0
100	405.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.405291971825462E-4
2	2.801763990608487E-4
3	4.669606651014145E-4
4	0.010459918898271685
5	0.0316599330938759
6	0.05855686740371738
7	0.10553311031291969
8	0.10805469790446731
9	0.1169269505413942
10	0.132710221021822
11	0.1382203568700187
12	0.13084237836141635
13	0.12589259531134137
14	0.11645998987629279
15	0.13299039742088287
16	0.11814104827065786
17	0.12402475265093571
18	0.11347144161964373
19	0.09955601379962158
20	0.09385909368538432
21	0.09413927008444517
22	0.1026379541892909
23	0.08293221412201121
24	0.0933921330202829
25	0.08965644769947158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1070754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.057949197325016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2723626405774	38.889395391733714
2	11.9498743241462	11.72472655016766
3	3.436950354989485	5.05829207726407
4	1.4552254136973304	2.8556149766327965
5	0.8275718738409762	2.0299489472012837
6	0.5420323886801236	1.5954598432304536
7	0.3945896408986852	1.3550430989898894
8	0.2935043995799234	1.1518979139026613
9	0.22813304719847238	1.0072565495724248
>10	1.363551873460125	12.343302711709574
>50	0.11658161565022429	3.9519200663901737
>100	0.10111624656314261	10.455166599464128
>500	0.0143089026169303	4.740138476923557
>1k	0.004197278100966221	2.841836796817651
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2011	0.1878115795037889	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1941	0.18127413019236913	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1634	0.15260274535514226	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1612	0.15054811842869603	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1593	0.14877366790131066	No Hit
TCGTAGTTCCGACCATAAACGATGC	1503	0.1403683759294852	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1500	0.14008819953042437	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1499	0.13999480739740408	No Hit
GAACTACGACGGTATCTGATCGTCT	1463	0.1366326906086739	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1442	0.13467145581524795	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1421	0.132710221021822	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1330	0.12421153691697627	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1256	0.11730051907347533	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1247	0.11645998987629279	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1233	0.11515250001400883	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1161	0.10842826643654845	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1157	0.10805469790446731	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1136	0.10609346311104138	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1090	0.10179742499210837	No Hit
GAATAATGGAATAGGACCGCGGTTC	1082	0.1010502879279461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.867842660405658E-4
13	9.33921330202829E-5	0.0	0.0	0.0	2.801763990608487E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTATAC	35	0.0021718878	16.28315	3
GGTATCA	490	0.0	14.341327	1
CCGTCCG	40	0.005276643	14.250422	9
CGTCAAT	260	0.0	13.885026	19
TCGCGTA	135	1.8189894E-12	13.370766	9
CGTCTTA	50	0.0014981522	13.301015	15
TCTCGTC	65	5.445483E-5	13.154851	12
CCGTCAA	275	0.0	13.12766	18
GTATCAA	1285	0.0	13.080484	1
CGCCAGT	290	0.0	12.77624	18
GCGAATC	60	4.0875393E-4	12.667633	15
TAAGCCT	60	4.0907657E-4	12.666449	4
CGCATCG	295	0.0	12.56028	13
CGCGTAA	145	7.2759576E-12	12.449225	10
ATCGCCA	300	0.0	12.350365	16
TCCGTCA	295	0.0	12.237078	17
CTATACC	70	1.0904618E-4	12.214075	4
GCATAAA	70	1.0942762E-4	12.209509	1
CTATTCC	235	0.0	12.127451	4
GGCACCG	55	0.003066691	12.091267	5
>>END_MODULE