FastQCFastQC Report
Thu 2 Feb 2017
SRR4062114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070754
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18830.1758573864771927No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16770.15661860707501443No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16310.1523225689560814No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG15520.14494459044747907No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15310.14298335565405312No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14880.13896749393418095No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14860.1387807096681404No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14310.13364414235202485No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA14040.13112255476047718No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12990.12131638079334749No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12750.1190749696008607No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11660.10889522710164988No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG11440.10684060017520365No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC10940.1021709935241895No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10910.10189081712512864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14100.026.5353281
GGTATCA5900.024.2468871
GTACGTA500.002581312621.99843611
CTTACAC2150.020.4627063
CGTCGTA4900.020.20358810
GAGTATA550.004473019420.0079211
CGTAGTC550.004485127519.99857914
GTATAAG1553.274181E-1119.878841
ACCGTCG4650.019.8695558
ATACCGT4650.019.8695556
CCGTCGT4800.019.2486329
TACCGTC4800.019.2486327
CTAGCGG4350.018.20560329
CGCAATA4150.018.02618236
GTCGTAG5150.017.94047411
GTATAGG1355.498805E-817.9330251
TAGCGGC4450.017.79648830
TCTAGCG4600.017.69439328
GCGCAAT4600.017.2185835
TATACTC902.2162775E-417.1098945
Produced by FastQC (version 0.11.5)