##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070754
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05548706799134	32.0	32.0	32.0	32.0	32.0
2	30.969110552003542	32.0	32.0	32.0	32.0	32.0
3	31.29850367124475	32.0	32.0	32.0	32.0	32.0
4	31.46092099585899	32.0	32.0	32.0	32.0	32.0
5	31.38379310280419	32.0	32.0	32.0	32.0	32.0
6	34.994461846511896	36.0	36.0	36.0	36.0	36.0
7	35.00594347534541	36.0	36.0	36.0	36.0	36.0
8	34.91749085224057	36.0	36.0	36.0	36.0	36.0
9	35.07381434017524	36.0	36.0	36.0	36.0	36.0
10	34.87034089996395	36.0	36.0	36.0	32.0	36.0
11	35.085078365338816	36.0	36.0	36.0	36.0	36.0
12	34.95294810946305	36.0	36.0	36.0	32.0	36.0
13	35.02855090898563	36.0	36.0	36.0	36.0	36.0
14	34.97186281816365	36.0	36.0	36.0	32.0	36.0
15	34.93275859814673	36.0	36.0	36.0	32.0	36.0
16	34.94158975824512	36.0	36.0	36.0	32.0	36.0
17	34.909135058099245	36.0	36.0	36.0	32.0	36.0
18	34.914144612114455	36.0	36.0	36.0	32.0	36.0
19	34.9043655218659	36.0	36.0	36.0	32.0	36.0
20	34.90776779727183	36.0	36.0	36.0	32.0	36.0
21	34.89984347478506	36.0	36.0	36.0	32.0	36.0
22	34.88085405237804	36.0	36.0	36.0	32.0	36.0
23	34.841860035078085	36.0	36.0	36.0	32.0	36.0
24	34.8181776579868	36.0	36.0	36.0	32.0	36.0
25	34.79594659464265	36.0	36.0	36.0	32.0	36.0
26	34.74414291237763	36.0	36.0	36.0	32.0	36.0
27	34.72767694540482	36.0	36.0	36.0	32.0	36.0
28	34.69690797325996	36.0	36.0	36.0	32.0	36.0
29	34.67271847688638	36.0	36.0	36.0	32.0	36.0
30	34.645752432398105	36.0	36.0	36.0	32.0	36.0
31	34.6608604777568	36.0	36.0	36.0	32.0	36.0
32	34.62137428391582	36.0	36.0	36.0	32.0	36.0
33	34.59147946213603	36.0	36.0	36.0	32.0	36.0
34	34.5863055379667	36.0	36.0	36.0	32.0	36.0
35	34.570893968175696	36.0	36.0	36.0	32.0	36.0
36	34.54628327328219	36.0	36.0	36.0	32.0	36.0
37	34.52288947788194	36.0	36.0	36.0	32.0	36.0
38	34.507242559915724	36.0	36.0	36.0	32.0	36.0
39	34.491264099877284	36.0	36.0	36.0	32.0	36.0
40	34.47249134721888	36.0	36.0	36.0	32.0	36.0
41	34.43497759522729	36.0	36.0	36.0	32.0	36.0
42	34.393442377987846	36.0	36.0	36.0	32.0	36.0
43	34.403851865134286	36.0	36.0	36.0	32.0	36.0
44	34.32949304882354	36.0	36.0	36.0	32.0	36.0
45	34.30636168531708	36.0	36.0	36.0	32.0	36.0
46	34.29615766086328	36.0	36.0	36.0	32.0	36.0
47	34.27481569062549	36.0	36.0	36.0	32.0	36.0
48	34.244615476570715	36.0	36.0	36.0	32.0	36.0
49	34.241286047028545	36.0	36.0	36.0	32.0	36.0
50	33.71438724487604	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	27.0
22	62.0
23	193.0
24	536.0
25	1299.0
26	2847.0
27	5518.0
28	9827.0
29	16525.0
30	25889.0
31	40829.0
32	65247.0
33	114386.0
34	244266.0
35	543294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.377523347592096	18.15054000502591	12.230990057480337	26.24094658990166
2	16.450223727749805	20.061248448475375	36.07391654782152	27.414611275953305
3	18.08339948634135	23.609806210600045	28.586971748774225	29.71982255428438
4	12.35951488390424	15.68418142729329	35.86108480566031	36.09521888314216
5	15.122707923575348	36.385294848303154	32.39717059193802	16.094826636183473
6	35.07466728928321	35.4169507354658	15.983095960775156	13.525286014475835
7	30.944455962807517	30.14006952110382	20.01907067356274	18.89640384252592
8	28.72984831249755	32.1184884670055	18.91993866004703	20.231724560449926
9	27.279468486692554	13.738169551549657	18.40142553751842	40.58093642423937
10	16.344479072204326	25.887949881998928	30.367414333651176	27.400156712145563
11	38.05748099003132	20.924227226795324	21.655394236211116	19.362897546962234
12	24.768247421910168	23.321416497159944	27.9279834583854	23.982352622544486
13	29.13171466088383	18.83681966165898	25.30740020583626	26.724065471620932
14	24.059774700818302	19.343098414761933	24.401496515539517	32.195630368880245
15	25.561053239119346	26.849771282666236	21.64988410036292	25.939291377851497
16	26.469670791501283	25.48148494046229	22.936633201027316	25.112211067009106
17	24.32696959338933	25.855892203064386	24.595752152221706	25.22138605132458
18	25.495795949205842	24.416088490996522	25.4694593430978	24.618656216699836
19	25.90595038636325	24.636284337952507	24.929162067104116	24.528603208580122
20	26.28374611138143	23.615810555749086	24.212493450870557	25.887949881998928
21	27.473910907640786	23.784734869073567	23.827601858129878	24.91375236515577
22	26.279051957779288	23.773994773776234	24.696709048016633	25.25024422042785
23	24.582303685066783	23.67294448584829	25.217090013205645	26.527661815879277
24	24.927667252551025	24.69231731895333	24.96605170600684	25.41396372248881
25	25.22012649101376	23.875365632249604	24.93976173665512	25.964746140081512
26	25.01779126780294	25.019845902404857	25.253327107167873	24.70903572262433
27	25.650058370301192	24.170628064440812	24.59304226009806	25.586271305159936
28	24.85759835029825	24.0378841155501	25.428792637608232	25.675724896543418
29	24.792247647901554	24.41167186242115	25.371750162970493	25.4243303267068
30	24.81963290568237	24.557102470924125	25.563702622409135	25.05956200098437
31	25.606422432189863	24.23261517426619	24.28640939456286	25.874552998981088
32	25.579738162881444	24.25196732733002	24.053600213683595	26.114694296104947
33	24.704646275974785	24.00588372636003	25.01685734298389	26.2726126546813
34	25.77100451460944	24.032310183741053	25.063367035692874	25.133318265956632
35	26.53168339948634	23.673126313331778	25.094559887929023	24.70063039925286
36	24.807541622655503	24.59507392475001	24.56425443450438	26.033130018090105
37	26.228171597533663	24.351055066692318	24.53840248501501	24.88237085075901
38	25.136212426010086	23.825080270538333	25.157225655939648	25.88148164751194
39	26.099982815687035	23.995091263644717	24.06578976860949	25.839136152058757
40	26.03532280553965	24.235094368168944	24.951319305199238	24.778263521092168
41	24.76560756182366	24.868713150725615	25.3152238577367	25.050455429714024
42	26.579023805721256	24.84279108515946	24.40523055283929	24.172954556279997
43	25.801471535922627	23.627229578656564	24.940460513412496	25.630838372008313
44	25.22977378921051	24.593290353189936	24.362138995280777	25.81479686231878
45	25.426392933392854	24.891895779125566	24.38728488894451	25.294426398537066
46	25.177449361555876	24.656775576990608	24.569638601026593	25.596136460426926
47	25.301263330721437	24.298126250886067	25.047233644737616	25.35337677365488
48	26.58724265883634	24.985220073801564	23.600997096316554	24.82654017104554
49	25.22106702430897	25.118238636575903	24.0367193541518	25.623974984963333
50	25.76311424579545	24.928834530009038	23.98397749602146	25.324073728174056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	46.0
2	26.0
3	70.0
4	114.0
5	134.0
6	154.0
7	123.5
8	93.0
9	128.0
10	163.0
11	259.0
12	355.0
13	685.5
14	1016.0
15	1335.0
16	1654.0
17	1689.5
18	1725.0
19	1752.5
20	1780.0
21	2204.5
22	2629.0
23	3184.5
24	3740.0
25	4886.0
26	6032.0
27	7479.5
28	8927.0
29	10281.0
30	11635.0
31	13019.5
32	14404.0
33	16188.0
34	17972.0
35	20993.0
36	24014.0
37	29910.0
38	35806.0
39	39974.0
40	44142.0
41	48810.0
42	53478.0
43	54762.5
44	56047.0
45	61825.0
46	67603.0
47	72557.5
48	77512.0
49	81321.0
50	85130.0
51	82144.5
52	79159.0
53	78345.5
54	77532.0
55	80392.5
56	83253.0
57	80986.0
58	78719.0
59	71725.5
60	64732.0
61	57340.5
62	49949.0
63	43327.5
64	36706.0
65	30887.5
66	25069.0
67	21598.5
68	18128.0
69	16794.5
70	15461.0
71	12199.5
72	8938.0
73	7994.0
74	7050.0
75	5078.5
76	3107.0
77	2785.0
78	2463.0
79	2112.5
80	1762.0
81	1390.0
82	1018.0
83	901.0
84	784.0
85	599.5
86	415.0
87	281.5
88	148.0
89	104.5
90	61.0
91	46.0
92	31.0
93	24.5
94	18.0
95	18.0
96	18.0
97	14.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02811103203910515
2	0.003081940389669336
3	3.735685320811316E-4
4	0.0
5	0.0
6	3.735685320811316E-4
7	0.0
8	0.0
9	0.0
10	9.33921330202829E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.735685320811316E-4
17	0.0
18	9.33921330202829E-5
19	0.0
20	9.33921330202829E-5
21	0.0
22	0.0
23	0.0
24	7.471370641622632E-4
25	5.603527981216974E-4
26	3.735685320811316E-4
27	3.735685320811316E-4
28	0.0014008819953042434
29	7.471370641622632E-4
30	0.0019612347934259413
31	6.537449311419803E-4
32	0.0011207055962433948
33	3.735685320811316E-4
34	7.471370641622632E-4
35	3.735685320811316E-4
36	2.801763990608487E-4
37	0.0014942741283245264
38	0.0
39	9.33921330202829E-4
40	9.33921330202829E-5
41	6.537449311419803E-4
42	0.0042026459859127304
43	0.003362116788730185
44	0.002521587591547638
45	0.002614979724567921
46	0.0024281954585273556
47	0.0012140977292636778
48	0.0042026459859127304
49	0.00354890105477075
50	9.33921330202829E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1070754.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.765412618583895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00068390117308	41.41268411913126
2	11.669596874218367	12.081629945729013
3	3.317895645370555	5.152567113240284
4	1.3986679105677668	2.8961048602765214
5	0.8002984402238985	2.07138894880996
6	0.5029507029347124	1.5621270398533318
7	0.3770983661364987	1.3664456764594777
8	0.26473254165076215	1.0963191401694519
9	0.21804905508225877	1.0158659376682908
>10	1.2474796958568088	11.83508414447872
>50	0.09792643248616725	3.515012969819562
>100	0.08920841236869988	9.513364308575476
>500	0.01232961754439313	4.303854684161609
>1k	0.0030824043860982826	2.1775511116271065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1883	0.1758573864771927	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1677	0.15661860707501443	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1631	0.1523225689560814	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1552	0.14494459044747907	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1531	0.14298335565405312	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1488	0.13896749393418095	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1486	0.1387807096681404	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1431	0.13364414235202485	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1404	0.13112255476047718	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1299	0.12131638079334749	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1275	0.1190749696008607	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1166	0.10889522710164988	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1144	0.10684060017520365	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	1094	0.1021709935241895	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1091	0.10189081712512864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.867842660405658E-4
2	0.0	0.0	0.0	0.0	1.867842660405658E-4
3	0.0	0.0	0.0	0.0	1.867842660405658E-4
4	0.0	0.0	0.0	0.0	1.867842660405658E-4
5	0.0	1.867842660405658E-4	0.0	0.0	1.867842660405658E-4
6	0.0	1.867842660405658E-4	0.0	0.0	1.867842660405658E-4
7	0.0	1.867842660405658E-4	0.0	0.0	1.867842660405658E-4
8	0.0	1.867842660405658E-4	0.0	0.0	1.867842660405658E-4
9	0.0	1.867842660405658E-4	0.0	9.33921330202829E-5	1.867842660405658E-4
10	0.0	1.867842660405658E-4	0.0	9.33921330202829E-5	1.867842660405658E-4
11	0.0	1.867842660405658E-4	0.0	9.33921330202829E-5	1.867842660405658E-4
12	0.0	1.867842660405658E-4	0.0	9.33921330202829E-5	2.801763990608487E-4
13	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	4.669606651014145E-4
14	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	4.669606651014145E-4
15	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	6.537449311419803E-4
16	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	7.471370641622632E-4
17	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	7.471370641622632E-4
18	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	7.471370641622632E-4
19	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	7.471370641622632E-4
20	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	7.471370641622632E-4
21	0.0	3.735685320811316E-4	0.0	9.33921330202829E-5	7.471370641622632E-4
22	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	7.471370641622632E-4
23	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	7.471370641622632E-4
24	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	7.471370641622632E-4
25	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	7.471370641622632E-4
26	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	8.405291971825461E-4
27	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	9.33921330202829E-4
28	0.0	3.735685320811316E-4	0.0	1.867842660405658E-4	9.33921330202829E-4
29	0.0	3.735685320811316E-4	9.33921330202829E-5	4.669606651014145E-4	9.33921330202829E-4
30	0.0	3.735685320811316E-4	9.33921330202829E-5	8.405291971825461E-4	9.33921330202829E-4
31	0.0	3.735685320811316E-4	9.33921330202829E-5	0.0022414111924867895	9.33921330202829E-4
32	0.0	3.735685320811316E-4	9.33921330202829E-5	0.003642293187791033	9.33921330202829E-4
33	0.0	3.735685320811316E-4	9.33921330202829E-5	0.005229959449135842	9.33921330202829E-4
34	0.0	3.735685320811316E-4	9.33921330202829E-5	0.0070978021095415	0.001027313463223112
35	0.0	3.735685320811316E-4	9.33921330202829E-5	0.009152429035987725	0.001027313463223112
36	0.0	3.735685320811316E-4	9.33921330202829E-5	0.011954193026596211	0.001027313463223112
37	0.0	3.735685320811316E-4	9.33921330202829E-5	0.015503094081366962	0.001027313463223112
38	0.0	3.735685320811316E-4	9.33921330202829E-5	0.021386798461644783	0.001027313463223112
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1410	0.0	26.535328	1
GGTATCA	590	0.0	24.246887	1
GTACGTA	50	0.0025813126	21.998436	11
CTTACAC	215	0.0	20.462706	3
CGTCGTA	490	0.0	20.203588	10
GAGTATA	55	0.0044730194	20.007921	1
CGTAGTC	55	0.0044851275	19.998579	14
GTATAAG	155	3.274181E-11	19.87884	1
ACCGTCG	465	0.0	19.869555	8
ATACCGT	465	0.0	19.869555	6
CCGTCGT	480	0.0	19.248632	9
TACCGTC	480	0.0	19.248632	7
CTAGCGG	435	0.0	18.205603	29
CGCAATA	415	0.0	18.026182	36
GTCGTAG	515	0.0	17.940474	11
GTATAGG	135	5.498805E-8	17.933025	1
TAGCGGC	445	0.0	17.796488	30
TCTAGCG	460	0.0	17.694393	28
GCGCAAT	460	0.0	17.21858	35
TATACTC	90	2.2162775E-4	17.109894	5
>>END_MODULE