FastQCFastQC Report
Thu 2 Feb 2017
SRR4062112_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062112_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035961
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21910.21149444815007512No Hit
TATCAACGCAGAGTACTTTTTTTTT14220.13726385452734224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.014.9347941
GAACGCG400.00527351714.2516317
GGACCGA801.2859527E-714.2509426
TTGACCG400.005281980614.24818818
CGTACAC551.9612696E-413.8164253
CGTGTCA655.443197E-513.15535310
TAGACTG957.375638E-813.0002325
ACCGTGC701.0895479E-412.2150948
GTCCTAT701.094969E-412.2086011
TAATACT951.0390286E-611.9996344
GACCGAG1353.6925485E-1011.9643347
AAGGGTA658.018241E-411.6925165
GTAATAC658.028002E-411.6908223
AGGACTG2050.011.5855725
GTATCAA12550.011.5762831
TAACCCT600.00587474111.0829944
GTCTAAC600.00589379211.07817651
TTAATAC951.3607829E-510.9986013
TAGACAG1303.2589014E-810.9617345
TATAGTG1053.461213E-610.8573365
Produced by FastQC (version 0.11.5)