##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062112_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1035961
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223618456679354	32.0	32.0	32.0	32.0	32.0
2	30.763164829564047	32.0	32.0	32.0	32.0	32.0
3	30.787563431441917	32.0	32.0	32.0	32.0	32.0
4	30.78304974801175	32.0	32.0	32.0	32.0	32.0
5	30.681456155202753	32.0	32.0	32.0	32.0	32.0
6	34.35193988962905	36.0	36.0	36.0	32.0	36.0
7	34.28049704573821	36.0	36.0	36.0	32.0	36.0
8	34.22549111404773	36.0	36.0	36.0	32.0	36.0
9	34.37349378982413	36.0	36.0	36.0	32.0	36.0
10	34.03959898104272	36.0	36.0	36.0	32.0	36.0
11	34.36015931101653	36.0	36.0	36.0	32.0	36.0
12	34.18510735442744	36.0	36.0	36.0	32.0	36.0
13	34.26103974956586	36.0	36.0	36.0	32.0	36.0
14	34.164317961776554	36.0	36.0	36.0	32.0	36.0
15	34.112525471518715	36.0	36.0	36.0	32.0	36.0
16	34.123067374157905	36.0	36.0	36.0	32.0	36.0
17	34.061085311126575	36.0	36.0	36.0	32.0	36.0
18	34.06476015989019	36.0	36.0	36.0	32.0	36.0
19	34.07161273445622	36.0	36.0	36.0	32.0	36.0
20	34.05148456360809	36.0	36.0	36.0	32.0	36.0
21	34.04159809104783	36.0	36.0	36.0	32.0	36.0
22	34.01317231054065	36.0	36.0	36.0	32.0	36.0
23	33.971869597407625	36.0	36.0	36.0	32.0	36.0
24	33.95447994663892	36.0	36.0	36.0	32.0	36.0
25	33.58374398264027	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	13.0
5	78.0
6	218.0
7	73.0
8	192.0
9	148.0
10	69.0
11	20.0
12	53.0
13	48.0
14	223.0
15	306.0
16	453.0
17	563.0
18	754.0
19	914.0
20	1444.0
21	2012.0
22	3039.0
23	4447.0
24	6270.0
25	8935.0
26	12329.0
27	15846.0
28	21302.0
29	28240.0
30	37054.0
31	50337.0
32	70621.0
33	99561.0
34	212779.0
35	457618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.922534871374104	17.349389449297746	12.08793860707563	25.64013707225252
2	16.545554935624804	18.998550134271852	38.07046231603984	26.385432614063504
3	19.49889521801707	22.386869649240168	28.148146003044545	29.96608912969822
4	13.430367600067191	15.175015011768068	34.20926115070271	37.18535623746204
5	15.069572522474678	36.025144600766694	33.43546315697994	15.469819719778682
6	35.30008355106516	33.95853355806799	16.27709976383543	14.464283127031427
7	30.9351217885841	30.085090139497577	19.592669008538007	19.387119063380315
8	27.981066214131918	33.97161091804508	18.579502186005698	19.4678206818173
9	26.779332087222596	15.450774437848777	18.539247655810556	39.230645819118074
10	16.796308236001465	27.286400733094318	30.9485857678786	24.96870526302562
11	36.31837766093709	22.006037950344965	21.622938411166437	20.05264597755151
12	24.10119544171992	23.774568323452677	28.58697764068697	23.537258594140432
13	29.71132023677424	20.078931190869266	24.696686165873423	25.513062406483066
14	23.19495116918512	19.22701859001928	25.374434011955216	32.20359622884038
15	24.914718985888154	27.336612789880533	22.18243998913507	25.566228235096244
16	25.31955343223341	26.441771928010226	23.332788864221325	24.90588577553504
17	23.95971065593418	26.38292854547001	25.114921864730487	24.54243893386532
18	24.250908187751087	26.067573614186184	25.4798971348718	24.201621063190927
19	25.48151855777788	25.52712669689816	25.186708319523664	23.804646425800293
20	25.341006482432505	25.090366597065948	25.46313786836285	24.1054890521387
21	25.422646325921505	24.91499728976866	25.192300817320746	24.470055566989092
22	25.64760906222671	24.42781286086322	25.15226918040866	24.772308896501407
23	24.644295639185785	24.969204654034215	25.28194044977146	25.104559257008535
24	24.53175287953259	25.12142781916893	25.328195699034854	25.018623602263624
25	24.794178942822278	24.99862318973605	25.396787056244392	24.810410811197283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	128.0
2	253.5
3	379.0
4	379.0
5	379.0
6	719.0
7	1059.0
8	1059.0
9	1059.0
10	1359.0
11	1659.0
12	1659.0
13	1659.0
14	2326.5
15	2994.0
16	2994.0
17	2994.0
18	5118.5
19	7243.0
20	7243.0
21	7243.0
22	10730.0
23	14217.0
24	14217.0
25	14217.0
26	19737.5
27	25258.0
28	25258.0
29	25258.0
30	33216.5
31	41175.0
32	41175.0
33	41175.0
34	50593.5
35	60012.0
36	60012.0
37	60012.0
38	72520.5
39	85029.0
40	85029.0
41	85029.0
42	96882.5
43	108736.0
44	108736.0
45	108736.0
46	122035.0
47	135334.0
48	135334.0
49	135334.0
50	140939.5
51	146545.0
52	146545.0
53	146545.0
54	140206.0
55	133867.0
56	133867.0
57	133867.0
58	121311.5
59	108756.0
60	108756.0
61	108756.0
62	93382.0
63	78008.0
64	78008.0
65	78008.0
66	62305.0
67	46602.0
68	46602.0
69	46602.0
70	34636.5
71	22671.0
72	22671.0
73	22671.0
74	16239.0
75	9807.0
76	9807.0
77	9807.0
78	6946.5
79	4086.0
80	4086.0
81	4086.0
82	2736.0
83	1386.0
84	1386.0
85	1386.0
86	912.5
87	439.0
88	439.0
89	439.0
90	287.5
91	136.0
92	136.0
93	136.0
94	105.0
95	74.0
96	74.0
97	74.0
98	217.5
99	361.0
100	361.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001061816033615165
2	2.895861909859541E-4
3	9.652873032865137E-4
4	0.010328574145165696
5	0.03388158434535663
6	0.06428813439888181
7	0.11351778686649401
8	0.1155448902033957
9	0.12307413116903049
10	0.13919442913391528
11	0.14517921041429166
12	0.1397736015158872
13	0.13407840642649674
14	0.12220537259607263
15	0.139001371673258
16	0.1268387516518479
17	0.1298311422920361
18	0.11660670623701085
19	0.10212739668771313
20	0.09749401763193788
21	0.09594955794667946
22	0.10772606304677493
23	0.08706891475644353
24	0.09498427064339295
25	0.09266758111550531
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1035961.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.021988102451644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.48201330696322	34.43066480077362
2	16.25581592123624	16.588080930579192
3	6.603146015959218	10.107169123950666
4	3.2809170261829195	6.695956379001439
5	1.8735614317733198	4.779641454057529
6	1.1337918840819288	3.470898961217064
7	0.7587755482397318	2.7099965896303178
8	0.5425027754158721	2.21436561222525
9	0.38284659589842623	1.7580235014894257
>10	1.6223850146659649	13.804445804200723
>50	0.049994653350623273	1.7100766815894421
>100	0.013299838105322323	1.1676493548721116
>500	3.7999525086262543E-4	0.11526233278673848
>1k	5.699928762939381E-4	0.44776847362653066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2191	0.21149444815007512	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1422	0.13726385452734224	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9305746065730273E-4
2	0.0	0.0	0.0	0.0	1.9305746065730273E-4
3	0.0	0.0	0.0	0.0	1.9305746065730273E-4
4	0.0	0.0	0.0	0.0	1.9305746065730273E-4
5	0.0	0.0	0.0	0.0	3.8611492131460545E-4
6	0.0	0.0	0.0	0.0	4.826436516432568E-4
7	0.0	0.0	0.0	0.0	4.826436516432568E-4
8	0.0	0.0	0.0	0.0	4.826436516432568E-4
9	0.0	0.0	0.0	0.0	4.826436516432568E-4
10	0.0	0.0	0.0	0.0	5.791723819719082E-4
11	0.0	0.0	0.0	0.0	5.791723819719082E-4
12	0.0	0.0	0.0	0.0	7.722298426292109E-4
13	0.0	0.0	0.0	0.0	8.687585729578623E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	515	0.0	14.934794	1
GAACGCG	40	0.005273517	14.251631	7
GGACCGA	80	1.2859527E-7	14.250942	6
TTGACCG	40	0.0052819806	14.248188	18
CGTACAC	55	1.9612696E-4	13.816425	3
CGTGTCA	65	5.443197E-5	13.155353	10
TAGACTG	95	7.375638E-8	13.000232	5
ACCGTGC	70	1.0895479E-4	12.215094	8
GTCCTAT	70	1.094969E-4	12.208601	1
TAATACT	95	1.0390286E-6	11.999634	4
GACCGAG	135	3.6925485E-10	11.964334	7
AAGGGTA	65	8.018241E-4	11.692516	5
GTAATAC	65	8.028002E-4	11.690822	3
AGGACTG	205	0.0	11.585572	5
GTATCAA	1255	0.0	11.576283	1
TAACCCT	60	0.005874741	11.082994	4
GTCTAAC	60	0.005893792	11.0781765	1
TTAATAC	95	1.3607829E-5	10.998601	3
TAGACAG	130	3.2589014E-8	10.961734	5
TATAGTG	105	3.461213E-6	10.857336	5
>>END_MODULE