FastQCFastQC Report
Thu 2 Feb 2017
SRR4062112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035961
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4900.032.334691
GATTGCG300.005741622329.33648740
GTATCAA12500.028.5191961
CCCTATA904.0783198E-722.0013032
TATCAAC18850.018.6748472
GTATAGG1456.2846084E-918.2114931
TATAGGG851.4299332E-418.1187212
ATCAACG19250.017.9430543
AACTATC750.00129102417.59934419
TCAACGC19650.017.5769524
CAACGCA19700.017.420675
GTCTTAG1451.2451164E-716.6938691
AACGCAG20850.016.6708416
CCTATAC1259.436597E-615.8401753
AATAACG850.00297837915.5303332
GTATGGA2254.2564352E-1014.6703671
GTAATAC1201.18064956E-414.6668263
TAAGGTA1201.1811347E-414.666124
GTATTGG2002.1067535E-814.3036091
AGGATAG1707.5905155E-714.2347645