##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1035961
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274155108155615	32.0	32.0	32.0	32.0	32.0
2	31.452440777210725	32.0	32.0	32.0	32.0	32.0
3	31.570130535802022	32.0	32.0	32.0	32.0	32.0
4	31.650637427470727	32.0	32.0	32.0	32.0	32.0
5	31.623264775411428	32.0	32.0	32.0	32.0	32.0
6	35.26683919568401	36.0	36.0	36.0	36.0	36.0
7	35.273564352326005	36.0	36.0	36.0	36.0	36.0
8	35.22320048727703	36.0	36.0	36.0	36.0	36.0
9	35.32329691947863	36.0	36.0	36.0	36.0	36.0
10	35.20076141862483	36.0	36.0	36.0	36.0	36.0
11	35.31746079244296	36.0	36.0	36.0	36.0	36.0
12	35.24370994660996	36.0	36.0	36.0	36.0	36.0
13	35.26733342278329	36.0	36.0	36.0	36.0	36.0
14	35.23842403333716	36.0	36.0	36.0	36.0	36.0
15	35.21236320672303	36.0	36.0	36.0	36.0	36.0
16	35.2282431481494	36.0	36.0	36.0	36.0	36.0
17	35.21867908154844	36.0	36.0	36.0	36.0	36.0
18	35.20778871019276	36.0	36.0	36.0	36.0	36.0
19	35.20037530370352	36.0	36.0	36.0	36.0	36.0
20	35.207135210688435	36.0	36.0	36.0	36.0	36.0
21	35.197099118596164	36.0	36.0	36.0	36.0	36.0
22	35.17862448489856	36.0	36.0	36.0	36.0	36.0
23	35.15191595050393	36.0	36.0	36.0	36.0	36.0
24	35.13890291236832	36.0	36.0	36.0	36.0	36.0
25	35.12787353964097	36.0	36.0	36.0	36.0	36.0
26	35.08806123010422	36.0	36.0	36.0	36.0	36.0
27	35.07126716160165	36.0	36.0	36.0	36.0	36.0
28	35.06195117383763	36.0	36.0	36.0	36.0	36.0
29	35.04240989766989	36.0	36.0	36.0	36.0	36.0
30	35.03010924156411	36.0	36.0	36.0	36.0	36.0
31	35.025750969389776	36.0	36.0	36.0	36.0	36.0
32	34.99631646365066	36.0	36.0	36.0	36.0	36.0
33	34.98417894109913	36.0	36.0	36.0	36.0	36.0
34	34.97566414179684	36.0	36.0	36.0	36.0	36.0
35	34.963428159940385	36.0	36.0	36.0	36.0	36.0
36	34.93164800605428	36.0	36.0	36.0	36.0	36.0
37	34.93186519569752	36.0	36.0	36.0	36.0	36.0
38	34.92762661914879	36.0	36.0	36.0	36.0	36.0
39	34.9006671100553	36.0	36.0	36.0	36.0	36.0
40	34.89363692262547	36.0	36.0	36.0	36.0	36.0
41	34.867247898328216	36.0	36.0	36.0	32.0	36.0
42	34.84488026093646	36.0	36.0	36.0	32.0	36.0
43	34.834658833681964	36.0	36.0	36.0	32.0	36.0
44	34.80328892689976	36.0	36.0	36.0	32.0	36.0
45	34.778725260893026	36.0	36.0	36.0	32.0	36.0
46	34.759934012959945	36.0	36.0	36.0	32.0	36.0
47	34.73216655839361	36.0	36.0	36.0	32.0	36.0
48	34.72000104251029	36.0	36.0	36.0	32.0	36.0
49	34.70007847785776	36.0	36.0	36.0	32.0	36.0
50	34.28992790269132	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	25.0
22	77.0
23	191.0
24	485.0
25	1065.0
26	2356.0
27	4430.0
28	7433.0
29	12099.0
30	18486.0
31	27262.0
32	42149.0
33	71353.0
34	161570.0
35	686974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.493471117552716	17.86952663550364	12.506119469423895	26.130882777519748
2	16.028276505845568	19.3595408108596	37.86707957135397	26.745103111940864
3	19.019768156847906	22.754665001221092	28.312606966105804	29.9129598758252
4	13.15561107030091	15.589776062998512	34.344632664743166	36.90998020195741
5	14.81667746179634	36.2469243533299	33.31776003150698	15.618638153366776
6	35.482161473415964	34.06386346963203	16.14232209737828	14.311652959573728
7	30.986205079148732	30.07893154278974	19.648809173318302	19.286054204743227
8	28.135518615083	33.986221489032886	18.467394042825937	19.410865853058176
9	26.86365606427269	15.192849923887097	18.53235787833712	39.41113613350309
10	16.742618689313595	27.437905480997838	30.91168489933501	24.907790930353553
11	36.441429744942134	21.82379452508347	21.703326669633316	20.031449060341075
12	24.178323315259938	23.640175643677704	28.63351033484851	23.547990706213845
13	29.741467101560776	19.902004032970353	24.787805718555042	25.56872314691383
14	23.196433070356896	19.044442792730614	25.44844834892433	32.31067578798816
15	25.049205520285028	27.18924747167123	22.204407308769344	25.557139699274394
16	25.397096597638708	26.28188235612096	23.39363506400362	24.927385982236714
17	24.14926816743101	26.182066699422084	25.163881651915464	24.50478348123144
18	24.303881045370566	25.92093501860114	25.659920575930684	24.11526336009761
19	25.541115930039837	25.42006890220771	25.314852586149478	23.723962581602976
20	25.465535731695425	24.866186530908134	25.566891289889444	24.101386447506993
21	25.497799142824046	24.672960345959304	25.29885323757674	24.53038727363991
22	25.6029908461805	24.30197661881094	25.35616688273014	24.738865652278417
23	24.83664925610134	24.735197560525926	25.53455197637749	24.893601206995246
24	24.73521837003402	24.778560313838145	25.386409356454188	25.09981195967365
25	24.88240342021918	24.693689023772222	25.580115777006192	24.843791779002412
26	24.61043192030542	25.108619582896942	25.56143847947063	24.719510017327007
27	24.897630589775222	25.12659828525205	25.27766676898781	24.698104355984917
28	25.125224071208553	24.628189002676788	25.438754834966787	24.807832091147873
29	24.682466596007156	25.294559410939094	25.347650571357416	24.67532342169633
30	24.96792783400663	25.089459382496177	25.098919344173677	24.843693439323516
31	24.74588738153477	24.840292753181114	25.440606665521187	24.973213199762924
32	24.846275771130927	25.159226446166112	25.31589484393974	24.678602938763216
33	24.708361447434594	25.12102336293881	25.28338531425532	24.887229875371276
34	25.052439738945182	24.93457702150005	25.331700051450312	24.681283188104455
35	24.87335418355921	24.968048959419285	25.614309432796635	24.544287424224873
36	24.80032974309769	25.017809602319787	25.369657843271636	24.812202811310883
37	25.0767903098448	24.83198801472675	25.314449320326123	24.776772355102334
38	24.779311190286123	25.05123262362193	25.380588651503288	24.788867534588658
39	25.026521575869083	25.10432463374162	25.07584832844088	24.793305461948417
40	25.17896443878142	24.845167767095255	25.168346268195684	24.80752152592764
41	24.68639926019667	25.048675082122614	25.457381671526935	24.807543986153785
42	24.959455859014508	24.863694459353532	25.322325920834672	24.85452376079729
43	24.894078533925278	24.84359147376845	25.337939915571734	24.92439007673454
44	24.644974326945075	25.161427960510117	25.305841911129427	24.887755801415373
45	24.856868432864996	24.967107009609677	25.34106869639504	24.834955861130283
46	24.987330261706113	24.88365560320986	25.096603737886525	25.032410397197502
47	24.775664415588427	25.26458857008267	25.335827667025757	24.623919347303147
48	24.76156459958298	25.25339794578732	25.15387288593714	24.83116456869256
49	24.919901729388993	24.946062176916058	25.03738241071903	25.096653682975923
50	24.635502940302022	25.391911563128648	25.26758099827405	24.70500449829528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	60.5
2	75.0
3	153.5
4	232.0
5	238.0
6	244.0
7	187.0
8	130.0
9	186.0
10	242.0
11	425.0
12	608.0
13	1092.5
14	1577.0
15	1974.0
16	2371.0
17	2270.5
18	2170.0
19	2056.5
20	1943.0
21	2246.5
22	2550.0
23	3234.5
24	3919.0
25	4960.0
26	6001.0
27	7515.5
28	9030.0
29	10651.5
30	12273.0
31	14685.0
32	17097.0
33	19381.5
34	21666.0
35	24887.0
36	28108.0
37	31106.5
38	34105.0
39	38706.5
40	43308.0
41	47590.5
42	51873.0
43	55250.0
44	58627.0
45	63510.0
46	68393.0
47	71950.0
48	75507.0
49	78251.0
50	80995.0
51	84045.0
52	87095.0
53	86304.0
54	85513.0
55	81740.0
56	77967.0
57	73999.0
58	70031.0
59	64844.0
60	59657.0
61	52000.5
62	44344.0
63	38202.0
64	32060.0
65	27040.5
66	22021.0
67	18099.0
68	14177.0
69	11233.0
70	8289.0
71	6629.0
72	4969.0
73	3932.0
74	2895.0
75	2330.0
76	1765.0
77	1382.0
78	999.0
79	792.5
80	586.0
81	444.0
82	302.0
83	202.5
84	103.0
85	79.5
86	56.0
87	34.0
88	12.0
89	13.5
90	15.0
91	10.0
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	3.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032047538469112256
2	0.0046333790557752655
3	1.9305746065730273E-4
4	0.0
5	0.0
6	9.652873032865136E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.8611492131460545E-4
17	0.0
18	2.895861909859541E-4
19	0.0
20	3.8611492131460545E-4
21	9.652873032865136E-5
22	0.0
23	0.0
24	0.0012548734942724677
25	3.8611492131460545E-4
26	5.791723819719082E-4
27	6.757011123005596E-4
28	0.0017375171459157244
29	6.757011123005596E-4
30	0.0015444596852584218
31	2.895861909859541E-4
32	0.0014479309549297705
33	3.8611492131460545E-4
34	9.652873032865137E-4
35	9.652873032865136E-5
36	2.895861909859541E-4
37	0.0022201607975589816
38	0.0
39	0.0011583447639438164
40	9.652873032865136E-5
41	9.652873032865137E-4
42	0.005116022707418523
43	0.005019493977089871
44	0.0048264365164325685
45	0.0025097469885449353
46	0.0027028044492022384
47	0.0012548734942724677
48	0.003957677943474706
49	0.004440321595117963
50	0.0012548734942724677
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1035961.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.91099862103565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.75776251261287	34.65462117735673
2	16.80112333936834	17.443261810036
3	6.835645645949613	10.645355751023363
4	3.4009136702948926	7.061792993957579
5	1.858527500895702	4.823900926807681
6	1.153065734539271	3.5914076253378937
7	0.7544080966457096	2.74134543652715
8	0.4959244560291891	2.0595147002455274
9	0.3613819882727771	1.6883729905405729
>10	1.5318237460630415	13.143833828785157
>50	0.04156447185036466	1.4344112415290367
>100	0.0072974924637221154	0.5012080606671201
>500	5.613450145136433E-4	0.21097345718624314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.895861909859541E-4
2	0.0	0.0	0.0	0.0	2.895861909859541E-4
3	0.0	0.0	0.0	0.0	2.895861909859541E-4
4	0.0	0.0	0.0	0.0	2.895861909859541E-4
5	0.0	0.0	0.0	0.0	3.8611492131460545E-4
6	0.0	0.0	0.0	0.0	3.8611492131460545E-4
7	0.0	0.0	0.0	0.0	3.8611492131460545E-4
8	0.0	0.0	0.0	0.0	3.8611492131460545E-4
9	0.0	0.0	0.0	0.0	3.8611492131460545E-4
10	0.0	0.0	0.0	0.0	3.8611492131460545E-4
11	0.0	0.0	0.0	0.0	3.8611492131460545E-4
12	0.0	0.0	0.0	0.0	9.652873032865136E-4
13	0.0	0.0	0.0	0.0	0.0011583447639438164
14	0.0	0.0	0.0	0.0	0.0011583447639438164
15	0.0	0.0	0.0	0.0	0.0013514022246011192
16	0.0	0.0	0.0	9.652873032865136E-5	0.0013514022246011192
17	0.0	0.0	0.0	9.652873032865136E-5	0.0013514022246011192
18	0.0	0.0	0.0	1.9305746065730273E-4	0.0015444596852584218
19	0.0	0.0	0.0	4.826436516432568E-4	0.0015444596852584218
20	0.0	0.0	0.0	6.757011123005596E-4	0.0019305746065730273
21	0.0	0.0	0.0	6.757011123005596E-4	0.00212363206723033
22	0.0	0.0	0.0	9.652873032865136E-4	0.00212363206723033
23	0.0	0.0	0.0	0.0011583447639438164	0.00212363206723033
24	0.0	0.0	0.0	0.0013514022246011192	0.00212363206723033
25	0.0	0.0	0.0	0.00212363206723033	0.00212363206723033
26	0.0	0.0	0.0	0.0027028044492022384	0.00212363206723033
27	0.0	0.0	0.0	0.003185448100845495	0.00212363206723033
28	0.0	0.0	0.0	0.0033785055615027978	0.00212363206723033
29	0.0	0.0	0.0	0.005791723819719082	0.00212363206723033
30	0.0	0.0	0.0	0.008784114459907275	0.00212363206723033
31	0.0	0.0	0.0	0.015348068122255568	0.00212363206723033
32	0.0	0.0	0.0	0.02249119416657577	0.0022201607975589816
33	0.0	0.0	0.0	0.030599607514182482	0.0022201607975589816
34	0.0	0.0	0.0	0.039769836895404365	0.0024132182582162843
35	0.0	0.0	0.0	0.0490365950069549	0.0024132182582162843
36	0.0	0.0	0.0	0.0629367321742807	0.0024132182582162843
37	0.0	0.0	0.0	0.08185636331869636	0.0024132182582162843
38	0.0	0.0	0.0	0.1099462238443339	0.0025097469885449358
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	490	0.0	32.33469	1
GATTGCG	30	0.0057416223	29.336487	40
GTATCAA	1250	0.0	28.519196	1
CCCTATA	90	4.0783198E-7	22.001303	2
TATCAAC	1885	0.0	18.674847	2
GTATAGG	145	6.2846084E-9	18.211493	1
TATAGGG	85	1.4299332E-4	18.118721	2
ATCAACG	1925	0.0	17.943054	3
AACTATC	75	0.001291024	17.599344	19
TCAACGC	1965	0.0	17.576952	4
CAACGCA	1970	0.0	17.42067	5
GTCTTAG	145	1.2451164E-7	16.693869	1
AACGCAG	2085	0.0	16.670841	6
CCTATAC	125	9.436597E-6	15.840175	3
AATAACG	85	0.002978379	15.530333	2
GTATGGA	225	4.2564352E-10	14.670367	1
GTAATAC	120	1.18064956E-4	14.666826	3
TAAGGTA	120	1.1811347E-4	14.66612	4
GTATTGG	200	2.1067535E-8	14.303609	1
AGGATAG	170	7.5905155E-7	14.234764	5
>>END_MODULE