FastQCFastQC Report
Thu 2 Feb 2017
SRR4062111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261142
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3460.132494964425485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG250.00601892219.010
TTCAAAT250.00601892219.02
CGCCGCA659.915311E-917.53846210
TAACGCC607.6126526E-817.4166664
AACGCCG607.6126526E-817.4166665
ACGCCGC651.8954415E-716.0769236
CGCATCG651.8954415E-716.07692313
AATAACG651.8954415E-716.0769232
GCATCGC704.3950058E-714.92857214
CGCCAGT704.3950058E-714.92857218
CGTCGTA456.7356764E-414.77777810
GTCCTAT400.005263531614.251
ACTTTCG400.005263531614.2515
TCGCCAG759.584201E-713.93333217
GCCGCAT759.584201E-713.93333211
ATAACGC759.584201E-713.9333323
CCGCATC759.584201E-713.93333212
CGACCAT551.950327E-413.81818210
CCGACCA500.001493720113.2999999
GGTATCA1153.7107384E-1013.2173911
Produced by FastQC (version 0.11.5)