##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062111_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261142
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.377951459359277	32.0	32.0	32.0	32.0	32.0
2	31.04346294353264	32.0	32.0	32.0	32.0	32.0
3	31.063938393670878	32.0	32.0	32.0	32.0	32.0
4	31.05709154406415	32.0	32.0	32.0	32.0	32.0
5	31.021651055747448	32.0	32.0	32.0	32.0	32.0
6	34.694380834948035	36.0	36.0	36.0	32.0	36.0
7	34.64137519050938	36.0	36.0	36.0	32.0	36.0
8	34.62457207189958	36.0	36.0	36.0	32.0	36.0
9	34.704589840010414	36.0	36.0	36.0	32.0	36.0
10	34.477563930735	36.0	36.0	36.0	32.0	36.0
11	34.645258135420576	36.0	36.0	36.0	32.0	36.0
12	34.56479616453883	36.0	36.0	36.0	32.0	36.0
13	34.616591739360196	36.0	36.0	36.0	32.0	36.0
14	34.55125946802889	36.0	36.0	36.0	32.0	36.0
15	34.51301207772017	36.0	36.0	36.0	32.0	36.0
16	34.49965918925336	36.0	36.0	36.0	32.0	36.0
17	34.461093964203386	36.0	36.0	36.0	32.0	36.0
18	34.458558944941835	36.0	36.0	36.0	32.0	36.0
19	34.46971762489373	36.0	36.0	36.0	32.0	36.0
20	34.44842269722986	36.0	36.0	36.0	32.0	36.0
21	34.41296689157623	36.0	36.0	36.0	32.0	36.0
22	34.413227286304	36.0	36.0	36.0	32.0	36.0
23	34.379150040973876	36.0	36.0	36.0	32.0	36.0
24	34.379678489097884	36.0	36.0	36.0	32.0	36.0
25	34.04494106654617	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	4.0
5	23.0
6	41.0
7	11.0
8	44.0
9	35.0
10	20.0
11	4.0
12	17.0
13	13.0
14	35.0
15	49.0
16	105.0
17	108.0
18	133.0
19	155.0
20	262.0
21	345.0
22	554.0
23	741.0
24	1094.0
25	1645.0
26	2307.0
27	3000.0
28	4144.0
29	5346.0
30	7286.0
31	9981.0
32	14646.0
33	21861.0
34	52005.0
35	135128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.039595619208086	17.462280768936203	12.164739220341579	26.33338439151413
2	16.6564551087148	19.460293633348904	37.14798845072796	26.735262807208336
3	18.92586352148273	22.687064409895076	28.468637512445433	29.918434556176766
4	13.41952878458286	15.63007445081038	34.55145684610436	36.39893991850241
5	15.50438966093125	35.82438291939265	33.04426432959995	15.62696309007615
6	34.92882732724103	34.265953982029615	16.50822844224764	14.296990248481714
7	30.70957601778732	30.243425592271716	20.17212297784252	18.874875412098444
8	28.102214964233962	32.76636689130651	19.258458495296363	19.872959649163157
9	27.072710683095515	14.434791943015313	18.62698491807301	39.865512455816166
10	16.878649953410967	26.533891123543373	30.635643101180637	25.951815821865022
11	36.93213846100751	21.04984642286056	22.297636714331183	19.720378401800744
12	24.84498487236417	23.34564251229959	28.191854467925193	23.617518147411047
13	29.428702887159236	19.50462022161727	24.992139871937425	26.07453701928607
14	23.91780244215692	19.439109015277857	24.74016140471946	31.902927137845765
15	25.427829734693802	26.93630580575399	21.76247061845985	25.873393841092362
16	25.823234201456113	25.636139876011672	23.487622253489807	25.053003669042408
17	24.285610217125416	25.982585490976433	24.883730738410456	24.848073553487694
18	25.0163887919831	24.951983714716178	25.556164677648756	24.475462815651966
19	25.761432021158342	24.9568783778604	24.96646096055809	24.315228640423168
20	26.170262511354796	24.397573044388142	24.46541434939422	24.966750094862842
21	26.56530271206709	24.300903000291292	24.19626841645331	24.937525871188313
22	26.316778019265342	24.25416757703645	24.474956206422036	24.954098197276174
23	24.840859530990713	24.436157940927362	25.06083937255924	25.66214315552268
24	25.206489618985927	24.668754239984057	24.814015614639587	25.31074052639043
25	24.956212128761255	24.606675686137738	25.003736820521468	25.43337536457954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	99.0
3	69.0
4	69.0
5	69.0
6	149.0
7	229.0
8	229.0
9	229.0
10	300.5
11	372.0
12	372.0
13	372.0
14	490.5
15	609.0
16	609.0
17	609.0
18	997.5
19	1386.0
20	1386.0
21	1386.0
22	2302.0
23	3218.0
24	3218.0
25	3218.0
26	4749.0
27	6280.0
28	6280.0
29	6280.0
30	8048.0
31	9816.0
32	9816.0
33	9816.0
34	12473.5
35	15131.0
36	15131.0
37	15131.0
38	17957.0
39	20783.0
40	20783.0
41	20783.0
42	24292.5
43	27802.0
44	27802.0
45	27802.0
46	31066.5
47	34331.0
48	34331.0
49	34331.0
50	35382.0
51	36433.0
52	36433.0
53	36433.0
54	34169.5
55	31906.0
56	31906.0
57	31906.0
58	29536.0
59	27166.0
60	27166.0
61	27166.0
62	23516.5
63	19867.0
64	19867.0
65	19867.0
66	16273.0
67	12679.0
68	12679.0
69	12679.0
70	9592.5
71	6506.0
72	6506.0
73	6506.0
74	4874.0
75	3242.0
76	3242.0
77	3242.0
78	2564.5
79	1887.0
80	1887.0
81	1887.0
82	1288.0
83	689.0
84	689.0
85	689.0
86	515.5
87	342.0
88	342.0
89	342.0
90	235.5
91	129.0
92	129.0
93	129.0
94	77.0
95	25.0
96	25.0
97	25.0
98	70.5
99	116.0
100	116.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.658668463900866E-4
2	0.0
3	7.658668463900866E-4
4	0.011488002695851299
5	0.028337073316433207
6	0.0601205474416218
7	0.1079872253410022
8	0.10722135849461213
9	0.114880026958513
10	0.1344096315414602
11	0.13823896577341063
12	0.13823896577341063
13	0.12713389650075438
14	0.1175605609208783
15	0.1336437646950701
16	0.11909229461365847
17	0.12445336253838908
18	0.11219949299614768
19	0.09649922264515091
20	0.09152108814361536
21	0.08960642102764013
22	0.09917975660751623
23	0.08003308544776405
24	0.0892234876044451
25	0.08616002021888475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	261142.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.84855963263551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.94989864146856	60.18577959686427
2	10.119597011427603	14.339177446447371
3	2.3908399663331483	5.08162703780527
4	0.8534134526267478	2.41852455558878
5	0.43648640304982933	1.5462216477655206
6	0.2406193702669843	1.0228521481876582
7	0.15490899270202207	0.7682555304956498
8	0.11993190627702963	0.6797602250979029
9	0.08976429228510155	0.5723703733358106
>10	0.558219448940958	7.663612753886154
>50	0.05863280238478415	2.8755254426938293
>100	0.02768771223725918	2.8462932418317735
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	346	0.132494964425485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.829334231950433E-4
13	0.0	0.0	0.0	0.0	3.829334231950433E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	25	0.006018922	19.0	10
TTCAAAT	25	0.006018922	19.0	2
CGCCGCA	65	9.915311E-9	17.538462	10
TAACGCC	60	7.6126526E-8	17.416666	4
AACGCCG	60	7.6126526E-8	17.416666	5
ACGCCGC	65	1.8954415E-7	16.076923	6
CGCATCG	65	1.8954415E-7	16.076923	13
AATAACG	65	1.8954415E-7	16.076923	2
GCATCGC	70	4.3950058E-7	14.928572	14
CGCCAGT	70	4.3950058E-7	14.928572	18
CGTCGTA	45	6.7356764E-4	14.777778	10
GTCCTAT	40	0.0052635316	14.25	1
ACTTTCG	40	0.0052635316	14.25	15
TCGCCAG	75	9.584201E-7	13.933332	17
GCCGCAT	75	9.584201E-7	13.933332	11
ATAACGC	75	9.584201E-7	13.933332	3
CCGCATC	75	9.584201E-7	13.933332	12
CGACCAT	55	1.950327E-4	13.818182	10
CCGACCA	50	0.0014937201	13.299999	9
GGTATCA	115	3.7107384E-10	13.217391	1
>>END_MODULE