FastQCFastQC Report
Thu 2 Feb 2017
SRR4062111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261142
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3020.11564589380490307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATATA250.002345514735.2119561
TATACTG454.0586834E-529.3320585
CGTATTG804.285539E-927.49880227
CGCTAGA804.285539E-927.49880237
CCTATTC508.319326E-526.3988513
TTCGTAT858.1599865E-925.88618325
CCGCTAG858.176357E-925.88122736
TATTGCG858.176357E-925.88122729
GTATCAA3600.024.4527451
GCCGCTA901.5017577E-824.44338235
TACGCTA450.00139563724.4433829
CGCTATT450.00139563724.44338211
TCGTATT901.5017577E-824.44338226
CGGTCCA450.00139563724.44338210
GGACGGC952.6660928E-823.15688710
ATTGCGC952.6660928E-823.15688730
GGTATCA2000.023.1078451
GTATTAT500.002569998622.0074731
ATTCGTA1004.5818524E-822.00325824
GCGCCGC1205.9299055E-1021.99904333
Produced by FastQC (version 0.11.5)