##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261142
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35444317650933	32.0	32.0	32.0	32.0	32.0
2	31.551033537309202	32.0	32.0	32.0	32.0	32.0
3	31.62879965689165	32.0	32.0	32.0	32.0	32.0
4	31.67811765246494	32.0	32.0	32.0	32.0	32.0
5	31.669777362507755	32.0	32.0	32.0	32.0	32.0
6	35.32540916436268	36.0	36.0	36.0	36.0	36.0
7	35.323253249190095	36.0	36.0	36.0	36.0	36.0
8	35.27948012958467	36.0	36.0	36.0	36.0	36.0
9	35.3491778419404	36.0	36.0	36.0	36.0	36.0
10	35.249335610510755	36.0	36.0	36.0	36.0	36.0
11	35.353187154881255	36.0	36.0	36.0	36.0	36.0
12	35.27262562130948	36.0	36.0	36.0	36.0	36.0
13	35.30702836004932	36.0	36.0	36.0	36.0	36.0
14	35.28172794877882	36.0	36.0	36.0	36.0	36.0
15	35.25977054629282	36.0	36.0	36.0	36.0	36.0
16	35.26844398832819	36.0	36.0	36.0	36.0	36.0
17	35.25082522152699	36.0	36.0	36.0	36.0	36.0
18	35.243652878510545	36.0	36.0	36.0	36.0	36.0
19	35.24180713941074	36.0	36.0	36.0	36.0	36.0
20	35.23913426411684	36.0	36.0	36.0	36.0	36.0
21	35.217747432431395	36.0	36.0	36.0	36.0	36.0
22	35.21232509515895	36.0	36.0	36.0	36.0	36.0
23	35.1747325210039	36.0	36.0	36.0	36.0	36.0
24	35.167996722089896	36.0	36.0	36.0	36.0	36.0
25	35.158120869105694	36.0	36.0	36.0	36.0	36.0
26	35.131361481492824	36.0	36.0	36.0	36.0	36.0
27	35.1020785626211	36.0	36.0	36.0	36.0	36.0
28	35.09104242136462	36.0	36.0	36.0	36.0	36.0
29	35.05447993811796	36.0	36.0	36.0	36.0	36.0
30	35.05176494014751	36.0	36.0	36.0	36.0	36.0
31	35.04940224092639	36.0	36.0	36.0	36.0	36.0
32	35.02790818788245	36.0	36.0	36.0	36.0	36.0
33	35.00812201790597	36.0	36.0	36.0	36.0	36.0
34	34.99606727374379	36.0	36.0	36.0	36.0	36.0
35	34.97600922103683	36.0	36.0	36.0	36.0	36.0
36	34.968963246050045	36.0	36.0	36.0	36.0	36.0
37	34.9472164569468	36.0	36.0	36.0	36.0	36.0
38	34.94023175130772	36.0	36.0	36.0	36.0	36.0
39	34.91618353233107	36.0	36.0	36.0	36.0	36.0
40	34.912354198099116	36.0	36.0	36.0	36.0	36.0
41	34.88949690206861	36.0	36.0	36.0	36.0	36.0
42	34.8554579500808	36.0	36.0	36.0	32.0	36.0
43	34.8656210031324	36.0	36.0	36.0	32.0	36.0
44	34.817107167747814	36.0	36.0	36.0	32.0	36.0
45	34.80802398694963	36.0	36.0	36.0	32.0	36.0
46	34.762722962985656	36.0	36.0	36.0	32.0	36.0
47	34.74183394475037	36.0	36.0	36.0	32.0	36.0
48	34.72648214381448	36.0	36.0	36.0	32.0	36.0
49	34.71915662742875	36.0	36.0	36.0	32.0	36.0
50	34.32835009305282	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	10.0
22	16.0
23	50.0
24	111.0
25	300.0
26	572.0
27	1117.0
28	1928.0
29	3036.0
30	4713.0
31	6605.0
32	10030.0
33	16432.0
34	38252.0
35	177970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09146766569369	17.969845319359212	12.461023389796747	26.47766362515035
2	16.085522914488575	19.837394637776068	37.239155493771136	26.837926953964224
3	18.18512533410941	23.338643343468306	28.75561954798539	29.720611774436893
4	13.108576942812721	16.059461901953725	34.86838578244787	35.96357537278569
5	15.002948587358603	36.2255018342511	32.893598119031026	15.877951459359277
6	34.89710578918749	34.55131690804237	16.5480849499506	14.003492352819539
7	30.58642424428089	30.248294031599666	20.270580756829617	18.894700967289825
8	28.14828713879805	33.126038706910414	19.09535808104403	19.630316073247506
9	27.57388700400548	14.315200159300304	18.52440434706022	39.58650848963399
10	16.648796440250898	26.599704375397295	30.945998728661035	25.805500455690773
11	37.05148922808281	21.020364399445512	22.263749224559817	19.664397147911863
12	24.841752156880766	23.42833947943831	28.341394112759005	23.388514250921915
13	29.48242718520958	19.535348584295136	25.021252804987327	25.96097142550796
14	23.880111203866093	19.298312795337402	24.791875684493494	32.02970031630301
15	25.321855542195433	27.171806909650687	21.854776328587512	25.651561219566364
16	25.800435783241877	25.772098384385327	23.405159704218825	25.02230612815397
17	24.397837192025797	25.648880685604002	24.9998085332884	24.953473589081803
18	25.09592482251036	24.929348783420515	25.83345459558401	24.141271798485114
19	25.945271155156963	24.583942835698586	25.146854967795303	24.32393104134915
20	26.222998303598438	24.213356003078797	24.755974741614683	24.80767095170808
21	26.724923604782074	23.975844559664857	24.49089001386219	24.80834182169088
22	26.43466007000023	23.949805086887594	24.790343950800715	24.825190892311465
23	24.837444761853707	24.258832359405993	25.233780854860573	25.669942023879727
24	25.266237521013714	24.55014379315231	25.092383749650573	25.091234936183408
25	25.383987960527072	24.112261192229486	25.169544422361867	25.33420642488158
26	24.99262850337557	25.027092643437836	25.423813189043464	24.556465664143126
27	25.357182518256423	24.458434332410462	24.88310912495548	25.301274024377634
28	25.048154062364773	24.550338705909837	25.145419524318278	25.25608770740711
29	25.1431219149808	24.746401369375164	25.05083460659184	25.0596421090522
30	25.038580690125258	24.73912560647313	25.119379951826794	25.102913751574818
31	25.549415832825943	24.462646616195848	24.71691538287745	25.27102216810076
32	25.417400628015624	24.71930765106839	24.344795894922264	25.51849582599372
33	24.852762098781504	24.55407402868937	25.116220293939694	25.476943578589427
34	25.45942613377448	24.46456129064375	25.1232093007226	24.952803274859175
35	25.721353598247692	24.5231503287496	25.359480127593905	24.396015945408802
36	24.85620849959026	24.734818604437432	24.900628776680886	25.508344119291422
37	25.53955732557249	24.592172780883818	24.71815884200046	25.150111051543234
38	25.069119482886705	24.505058550520406	25.102817624127873	25.323004342465016
39	25.414914719420384	24.401274421953143	24.721028728105445	25.462782130521028
40	25.873279671596293	24.387497989599527	25.038101875607904	24.70112046319627
41	24.91374391612194	24.911829241674038	25.084149941985363	25.090276900218655
42	25.89448127170862	24.95586472586346	24.65639588096305	24.49325812146487
43	25.448717703239343	24.30255846637537	25.04738964435747	25.201334186027808
44	24.954907690009996	24.775304349158088	24.88291317384761	25.386874786984308
45	25.197691650806657	24.93154986424854	24.87564094218022	24.995117542764582
46	25.145518044237487	24.525151645119784	24.967449911157406	25.361880399485326
47	25.31008152683799	24.423970192348197	25.209369650878262	25.05657862993555
48	25.661656294877933	25.15844901866922	24.259262805169936	24.920631881282908
49	25.10694030107955	25.1670643020446	24.355964722146695	25.370030674729154
50	25.437125198171085	25.15106954943363	24.51002917997381	24.901776072421477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	29.5
2	14.0
3	31.0
4	48.0
5	50.0
6	52.0
7	39.0
8	26.0
9	42.0
10	58.0
11	81.5
12	105.0
13	208.0
14	311.0
15	409.0
16	507.0
17	481.0
18	455.0
19	420.5
20	386.0
21	485.0
22	584.0
23	664.0
24	744.0
25	946.0
26	1148.0
27	1511.0
28	1874.0
29	2252.0
30	2630.0
31	3188.5
32	3747.0
33	4381.0
34	5015.0
35	5966.0
36	6917.0
37	8071.0
38	9225.0
39	10073.5
40	10922.0
41	11987.5
42	13053.0
43	13777.0
44	14501.0
45	15828.5
46	17156.0
47	18347.5
48	19539.0
49	20231.5
50	20924.0
51	20727.0
52	20530.0
53	20228.5
54	19927.0
55	19736.5
56	19546.0
57	18982.0
58	18418.0
59	16845.0
60	15272.0
61	13399.5
62	11527.0
63	9923.5
64	8320.0
65	7027.5
66	5735.0
67	4853.0
68	3971.0
69	3494.0
70	3017.0
71	2369.5
72	1722.0
73	1509.5
74	1297.0
75	982.5
76	668.0
77	569.0
78	470.0
79	402.0
80	334.0
81	250.5
82	167.0
83	151.0
84	135.0
85	98.0
86	61.0
87	42.5
88	24.0
89	14.5
90	5.0
91	5.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03369814124116381
2	0.007275735040705823
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	3.829334231950433E-4
13	0.0
14	0.0
15	0.0
16	0.00114880026958513
17	0.0
18	0.0
19	0.0
20	3.829334231950433E-4
21	0.0
22	0.0
23	0.0
24	0.00114880026958513
25	3.829334231950433E-4
26	3.829334231950433E-4
27	3.829334231950433E-4
28	3.829334231950433E-4
29	3.829334231950433E-4
30	3.829334231950433E-4
31	3.829334231950433E-4
32	7.658668463900866E-4
33	0.0
34	3.829334231950433E-4
35	3.829334231950433E-4
36	0.0
37	7.658668463900866E-4
38	0.0
39	0.0015317336927801733
40	0.0
41	3.829334231950433E-4
42	0.0049781345015355635
43	0.0034464008087553896
44	0.0042122676551454765
45	0.00114880026958513
46	0.00229760053917026
47	3.829334231950433E-4
48	0.006509868194315736
49	0.0057440013479256495
50	0.0015317336927801733
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	261142.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.84478469230875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.40307607782289	62.21168688949873
2	10.021021699131518	14.59958336140379
3	2.230939259310345	4.875368700182426
4	0.8110483165644305	2.363225599807816
5	0.4049618281635772	1.4749678590589756
6	0.2410305632532338	1.0534691690668632
7	0.147107930074374	0.7501231844959291
8	0.1087408642940051	0.6336963877401892
9	0.08942313516586238	0.5862608124901622
>10	0.4719340745143079	6.672816825605264
>50	0.05013465605985473	2.557202610670318
>100	0.020581595645624572	2.22159859997952
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	302	0.11564589380490307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.829334231950433E-4	0.0
5	0.0	0.0	0.0	3.829334231950433E-4	0.0
6	0.0	0.0	0.0	3.829334231950433E-4	0.0
7	0.0	0.0	0.0	3.829334231950433E-4	0.0
8	0.0	0.0	0.0	3.829334231950433E-4	0.0
9	0.0	0.0	0.0	3.829334231950433E-4	0.0
10	0.0	0.0	0.0	3.829334231950433E-4	0.0
11	0.0	0.0	0.0	3.829334231950433E-4	0.0
12	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
13	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
14	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
15	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
16	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
17	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
18	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
19	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
20	0.0	0.0	0.0	3.829334231950433E-4	3.829334231950433E-4
21	0.0	0.0	0.0	7.658668463900866E-4	3.829334231950433E-4
22	0.0	0.0	0.0	7.658668463900866E-4	3.829334231950433E-4
23	0.0	0.0	0.0	7.658668463900866E-4	7.658668463900866E-4
24	0.0	0.0	0.0	7.658668463900866E-4	7.658668463900866E-4
25	0.0	0.0	0.0	7.658668463900866E-4	7.658668463900866E-4
26	0.0	0.0	0.0	7.658668463900866E-4	7.658668463900866E-4
27	0.0	0.0	0.0	7.658668463900866E-4	0.00114880026958513
28	0.0	0.0	0.0	7.658668463900866E-4	0.00114880026958513
29	0.0	0.0	0.0	0.002680533962365303	0.00114880026958513
30	0.0	0.0	0.0	0.0065098681943157365	0.00114880026958513
31	0.0	0.0	0.0	0.01263680296543643	0.00114880026958513
32	0.0	0.0	0.0	0.023741872238092685	0.00114880026958513
33	0.0	0.0	0.0	0.03331520781796877	0.00114880026958513
34	0.0	0.0	0.0	0.041356809705064675	0.00114880026958513
35	0.0	0.0	0.0	0.05093014528494076	0.00114880026958513
36	0.0	0.0	0.0	0.06739628248232762	0.00114880026958513
37	0.0	0.0	0.0	0.09037228787403022	0.00114880026958513
38	0.0	0.0	0.0	0.12253869542241386	0.00114880026958513
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATATA	25	0.0023455147	35.211956	1
TATACTG	45	4.0586834E-5	29.332058	5
CGTATTG	80	4.285539E-9	27.498802	27
CGCTAGA	80	4.285539E-9	27.498802	37
CCTATTC	50	8.319326E-5	26.398851	3
TTCGTAT	85	8.1599865E-9	25.886183	25
CCGCTAG	85	8.176357E-9	25.881227	36
TATTGCG	85	8.176357E-9	25.881227	29
GTATCAA	360	0.0	24.452745	1
GCCGCTA	90	1.5017577E-8	24.443382	35
TACGCTA	45	0.001395637	24.443382	9
CGCTATT	45	0.001395637	24.443382	11
TCGTATT	90	1.5017577E-8	24.443382	26
CGGTCCA	45	0.001395637	24.443382	10
GGACGGC	95	2.6660928E-8	23.156887	10
ATTGCGC	95	2.6660928E-8	23.156887	30
GGTATCA	200	0.0	23.107845	1
GTATTAT	50	0.0025699986	22.007473	1
ATTCGTA	100	4.5818524E-8	22.003258	24
GCGCCGC	120	5.9299055E-10	21.999043	33
>>END_MODULE