FastQCFastQC Report
Thu 2 Feb 2017
SRR4062110_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062110_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257636
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18600.1478965296794939No Hit
TATCAACGCAGAGTACTTTTTTTTT14350.11410296779036223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT307.7186665E-419.0010764
GCTTAAC400.00528893314.2457021
GGTATCA5700.013.6625441
CCGATAA500.001497730513.3018129
CGATAAC500.001499079213.30022210
TCCAACG500.00149952913.29969418
CGACCCG655.4439028E-513.1556395
CAAGACG1850.012.8385644
AAGACGG2000.012.3516835
GTCCTAA853.9549304E-612.290411
AATACGA550.003064754412.0925565
CGAACGA550.00306932712.0901516
CGGACCA1701.8189894E-1211.7368939
CTCGCAC658.020414E-411.6925043
CGCAAGA1950.011.6906412
GTCTAGA1406.82121E-1011.5322351
GACGGAC1751.8189894E-1211.4015537
AGGGGCG752.0705893E-411.4015535
TCTAGGG1251.8293576E-811.4001913
TCTTACA1001.9317267E-611.39837552
Produced by FastQC (version 0.11.5)