##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062110_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1257636
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16967946210191	32.0	32.0	32.0	32.0	32.0
2	30.62707094898683	32.0	32.0	32.0	32.0	32.0
3	30.65366369919436	32.0	32.0	32.0	32.0	32.0
4	30.665117728818196	32.0	32.0	32.0	32.0	32.0
5	30.54763222426839	32.0	32.0	32.0	32.0	32.0
6	34.200029261248886	36.0	36.0	36.0	32.0	36.0
7	34.13197379845997	36.0	36.0	36.0	32.0	36.0
8	34.067182396178225	36.0	36.0	36.0	32.0	36.0
9	34.234612399772274	36.0	36.0	36.0	32.0	36.0
10	33.86956559767691	36.0	36.0	36.0	32.0	36.0
11	34.22479079797334	36.0	36.0	36.0	32.0	36.0
12	34.014333241096786	36.0	36.0	36.0	32.0	36.0
13	34.122222964355345	36.0	36.0	36.0	32.0	36.0
14	33.9991436313846	36.0	36.0	36.0	32.0	36.0
15	33.949558536810336	36.0	36.0	36.0	32.0	36.0
16	33.96087977761451	36.0	36.0	36.0	32.0	36.0
17	33.896320556981514	36.0	36.0	36.0	32.0	36.0
18	33.89160218059916	36.0	36.0	36.0	32.0	36.0
19	33.89807305134395	36.0	36.0	36.0	32.0	36.0
20	33.876942135880334	36.0	36.0	36.0	32.0	36.0
21	33.86535372715158	36.0	36.0	36.0	32.0	36.0
22	33.82020155275453	36.0	36.0	36.0	32.0	36.0
23	33.78225178032435	36.0	36.0	36.0	32.0	36.0
24	33.7586750061226	36.0	36.0	36.0	32.0	36.0
25	33.390739450842695	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	24.0
5	123.0
6	258.0
7	66.0
8	224.0
9	144.0
10	81.0
11	28.0
12	57.0
13	57.0
14	145.0
15	239.0
16	419.0
17	554.0
18	737.0
19	1105.0
20	1775.0
21	2460.0
22	3980.0
23	5992.0
24	8760.0
25	12380.0
26	17160.0
27	22201.0
28	29233.0
29	38233.0
30	50168.0
31	67294.0
32	93575.0
33	128495.0
34	263545.0
35	508122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16357061183726	17.176146727713096	11.850142570661136	25.810140089788508
2	16.753151955420982	18.90584845169334	37.860121243734255	26.48087834915142
3	19.931553741206667	21.893658622693973	27.788163281858164	30.386624354241192
4	13.25792819573208	15.296857032431685	34.33589879393132	37.10931597790491
5	15.35288357749264	35.716143627239255	33.267471171384	15.663501623884102
6	35.67358669368795	34.00480749721313	16.192322167559023	14.129283641539894
7	31.389576978673816	29.536271706667854	19.31113368187115	19.76301763278718
8	28.06381279965484	33.97909789315233	18.437126115533932	19.5199631916589
9	26.73385164321641	15.101493948893987	18.60311081461054	39.561543593279055
10	17.05782547180703	26.890950333965247	30.560388035055002	25.49083615917272
11	37.00705958043829	21.494057562732806	21.39715355423519	20.101729302593714
12	24.259214436082562	23.529177587892647	28.531550267364352	23.680057708660442
13	29.91111521582665	19.472567053713032	24.608840071082348	26.007477659377965
14	23.385276732939282	19.413537126594623	24.869862877385714	32.33132326308038
15	25.27355372624643	27.067706288929106	21.846797174134075	25.81194281069039
16	25.70532391282715	25.98308289382028	23.52218131157329	24.78941188177928
17	24.288797581676917	26.012019963744258	24.90022506582001	24.79895738875881
18	24.62997561695939	25.61962615855265	25.55124538190942	24.199152842578535
19	25.54056011627037	25.210485346017375	24.914954209786515	24.334000327925743
20	25.715722861984137	24.802360924408873	24.92970312553474	24.552213088072254
21	26.042370513980647	24.588873581660614	24.713586372163025	24.655169532195718
22	25.86710263234976	24.44005259605466	24.904884556117317	24.787960215478265
23	25.013747140157168	24.577578832189396	25.063881428429326	25.344792599224114
24	24.742016971248205	24.992001451676828	25.296742163412333	24.969239413662635
25	25.096358243355198	24.621709832559894	25.04836813743475	25.233563786650155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	58.0
2	189.5
3	321.0
4	321.0
5	321.0
6	692.5
7	1064.0
8	1064.0
9	1064.0
10	1349.0
11	1634.0
12	1634.0
13	1634.0
14	2261.5
15	2889.0
16	2889.0
17	2889.0
18	5246.0
19	7603.0
20	7603.0
21	7603.0
22	11642.0
23	15681.0
24	15681.0
25	15681.0
26	23492.5
27	31304.0
28	31304.0
29	31304.0
30	41032.0
31	50760.0
32	50760.0
33	50760.0
34	61996.0
35	73232.0
36	73232.0
37	73232.0
38	87239.0
39	101246.0
40	101246.0
41	101246.0
42	116798.0
43	132350.0
44	132350.0
45	132350.0
46	146041.5
47	159733.0
48	159733.0
49	159733.0
50	165205.0
51	170677.0
52	170677.0
53	170677.0
54	163781.5
55	156886.0
56	156886.0
57	156886.0
58	144256.5
59	131627.0
60	131627.0
61	131627.0
62	114528.5
63	97430.0
64	97430.0
65	97430.0
66	79686.0
67	61942.0
68	61942.0
69	61942.0
70	47327.5
71	32713.0
72	32713.0
73	32713.0
74	24036.5
75	15360.0
76	15360.0
77	15360.0
78	11547.0
79	7734.0
80	7734.0
81	7734.0
82	5438.5
83	3143.0
84	3143.0
85	3143.0
86	2207.0
87	1271.0
88	1271.0
89	1271.0
90	832.5
91	394.0
92	394.0
93	394.0
94	265.0
95	136.0
96	136.0
97	136.0
98	292.0
99	448.0
100	448.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011131996857596316
2	3.180570530741804E-4
3	5.565998428798158E-4
4	0.01288131064950431
5	0.036974132419873476
6	0.06750760951499479
7	0.10654911277985044
8	0.11124045431269462
9	0.11943042342935477
10	0.1325502768686647
11	0.1394680177730281
12	0.13294784818500743
13	0.12944922060119146
14	0.1161703386353444
15	0.1325502768686647
16	0.12014605179877166
17	0.12396273643566183
18	0.11410296779036223
19	0.09971088613875558
20	0.09374731639361468
21	0.09136188849555833
22	0.10082408582451521
23	0.08038892016449911
24	0.09207751686497523
25	0.08961257470365035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1257636.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.17648071002282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54492963224124	37.51331089778793
2	15.79794216341808	16.801579334221152
3	5.934800458248778	9.46775406257702
4	2.7477726123494586	5.844675092645199
5	1.4771393690183883	3.92745365813108
6	0.8892977031900331	2.837383329549144
7	0.5967095563123057	2.221163994750932
8	0.4031880825167121	1.7152098633968826
9	0.2803073940266349	1.3415184658200705
>10	1.2202885303394588	11.153413292093902
>50	0.0656379326455818	2.4335016583675335
>100	0.040337101383205495	4.024350324446546
>500	0.0013495617083162253	0.45594659912165214
>1k	2.999026018480501E-4	0.2627394270909136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1860	0.1478965296794939	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1435	0.11410296779036223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.590285265370902E-4
2	0.0	0.0	0.0	0.0	1.590285265370902E-4
3	0.0	0.0	0.0	0.0	1.590285265370902E-4
4	0.0	0.0	0.0	0.0	1.590285265370902E-4
5	0.0	0.0	0.0	0.0	1.590285265370902E-4
6	0.0	0.0	0.0	0.0	2.3854278980563534E-4
7	0.0	0.0	0.0	0.0	2.3854278980563534E-4
8	0.0	0.0	0.0	0.0	2.3854278980563534E-4
9	0.0	0.0	0.0	0.0	3.180570530741804E-4
10	0.0	0.0	0.0	0.0	3.9757131634272555E-4
11	0.0	0.0	0.0	0.0	4.770855796112707E-4
12	0.0	0.0	0.0	0.0	9.541711592225414E-4
13	0.0	0.0	0.0	0.0	0.001431256738833812
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGT	30	7.7186665E-4	19.001076	4
GCTTAAC	40	0.005288933	14.245702	1
GGTATCA	570	0.0	13.662544	1
CCGATAA	50	0.0014977305	13.301812	9
CGATAAC	50	0.0014990792	13.300222	10
TCCAACG	50	0.001499529	13.299694	18
CGACCCG	65	5.4439028E-5	13.155639	5
CAAGACG	185	0.0	12.838564	4
AAGACGG	200	0.0	12.351683	5
GTCCTAA	85	3.9549304E-6	12.29041	1
AATACGA	55	0.0030647544	12.092556	5
CGAACGA	55	0.003069327	12.09015	16
CGGACCA	170	1.8189894E-12	11.736893	9
CTCGCAC	65	8.020414E-4	11.692504	3
CGCAAGA	195	0.0	11.690641	2
GTCTAGA	140	6.82121E-10	11.532235	1
GACGGAC	175	1.8189894E-12	11.401553	7
AGGGGCG	75	2.0705893E-4	11.401553	5
TCTAGGG	125	1.8293576E-8	11.400191	3
TCTTACA	100	1.9317267E-6	11.3983755	2
>>END_MODULE