FastQCFastQC Report
Thu 2 Feb 2017
SRR4062110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257636
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG300.005745657729.3327524
GGTATCA6750.024.4507621
ATCGATT952.6861017E-823.15743629
GTATCAA12650.022.78851
CTATAGG1352.590241E-919.5606121
GTTATAG1950.019.1844441
TAGGACG1456.3082553E-918.2065354
GTATAGA1701.2551027E-1018.122331
TTGCGAG750.001291081817.59965111
ATTGCGA750.001291081817.59965110
GATATAC3400.017.4751051
ACCGTCG1802.8558134E-1017.1107718
GCATATA2500.016.7243211
CCTAATA1351.0853037E-616.2966212
ACGGACC3600.015.8885738
GACGGAC3050.015.8685377
CCTACAC2500.015.8403143
CGGATCG1401.5865808E-615.71397226
TACCGTC2252.5465852E-1115.6441337
CGTCGTA1857.568815E-915.45915110