##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1257636
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221550591745146	32.0	32.0	32.0	32.0	32.0
2	31.37157571825234	32.0	32.0	32.0	32.0	32.0
3	31.525007235797958	32.0	32.0	32.0	32.0	32.0
4	31.6125031408134	32.0	32.0	32.0	32.0	32.0
5	31.587014843722667	32.0	32.0	32.0	32.0	32.0
6	35.2047150367833	36.0	36.0	36.0	36.0	36.0
7	35.21605933672382	36.0	36.0	36.0	36.0	36.0
8	35.1591064505151	36.0	36.0	36.0	36.0	36.0
9	35.260308229090136	36.0	36.0	36.0	36.0	36.0
10	35.128048974425035	36.0	36.0	36.0	36.0	36.0
11	35.25038882474738	36.0	36.0	36.0	36.0	36.0
12	35.170462677595104	36.0	36.0	36.0	36.0	36.0
13	35.20359150024331	36.0	36.0	36.0	36.0	36.0
14	35.17347467788772	36.0	36.0	36.0	36.0	36.0
15	35.14403929276833	36.0	36.0	36.0	36.0	36.0
16	35.1609774211298	36.0	36.0	36.0	36.0	36.0
17	35.144902817667436	36.0	36.0	36.0	36.0	36.0
18	35.13523547353925	36.0	36.0	36.0	36.0	36.0
19	35.12956451628293	36.0	36.0	36.0	36.0	36.0
20	35.12963607911987	36.0	36.0	36.0	36.0	36.0
21	35.11857882566975	36.0	36.0	36.0	36.0	36.0
22	35.09673625754988	36.0	36.0	36.0	36.0	36.0
23	35.06860411120547	36.0	36.0	36.0	36.0	36.0
24	35.04868737854196	36.0	36.0	36.0	36.0	36.0
25	35.033075548091816	36.0	36.0	36.0	36.0	36.0
26	34.98426889815495	36.0	36.0	36.0	36.0	36.0
27	34.97951235492623	36.0	36.0	36.0	36.0	36.0
28	34.95207516324278	36.0	36.0	36.0	36.0	36.0
29	34.936466513363165	36.0	36.0	36.0	32.0	36.0
30	34.91337318588209	36.0	36.0	36.0	32.0	36.0
31	34.910807260606404	36.0	36.0	36.0	32.0	36.0
32	34.883300096371286	36.0	36.0	36.0	32.0	36.0
33	34.86657268080749	36.0	36.0	36.0	32.0	36.0
34	34.849810278967844	36.0	36.0	36.0	32.0	36.0
35	34.84532408423423	36.0	36.0	36.0	32.0	36.0
36	34.810754463135595	36.0	36.0	36.0	32.0	36.0
37	34.802325156086496	36.0	36.0	36.0	32.0	36.0
38	34.785441097424055	36.0	36.0	36.0	32.0	36.0
39	34.763581036166265	36.0	36.0	36.0	32.0	36.0
40	34.75452992757841	36.0	36.0	36.0	32.0	36.0
41	34.72996638136949	36.0	36.0	36.0	32.0	36.0
42	34.708119042393825	36.0	36.0	36.0	32.0	36.0
43	34.69074040501385	36.0	36.0	36.0	32.0	36.0
44	34.6574644809786	36.0	36.0	36.0	32.0	36.0
45	34.64173019856302	36.0	36.0	36.0	32.0	36.0
46	34.6163468602998	36.0	36.0	36.0	32.0	36.0
47	34.592471907610786	36.0	36.0	36.0	32.0	36.0
48	34.56283853197586	36.0	36.0	36.0	32.0	36.0
49	34.54027874520131	36.0	36.0	36.0	32.0	36.0
50	34.113775369025696	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	7.0
21	27.0
22	81.0
23	209.0
24	599.0
25	1434.0
26	3146.0
27	5958.0
28	10334.0
29	16739.0
30	25361.0
31	37806.0
32	57196.0
33	96254.0
34	215559.0
35	786923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82256886834323	17.653871055490775	12.275562455955598	26.247997620210395
2	16.116363266244107	19.37261993083536	37.54062369044012	26.970393112480405
3	19.210022025556007	22.345204869476714	28.093556928508384	30.351216176458895
4	13.055685428852225	15.739927928271774	34.41011548651597	36.794271156360026
5	15.009350877360381	36.08738935590266	33.11951947940421	15.783740287332742
6	35.73495945561268	34.203194252063795	16.109694871480894	13.952151420842629
7	31.408770105181468	29.533903291572443	19.361723105890736	19.695603497355354
8	28.09986355352423	34.061922527663015	18.30179797652103	19.53641594229173
9	26.758457932183877	14.85747863451746	18.55854953261516	39.825513900683504
10	17.033413536847764	26.904329876577766	30.61526644476851	25.446990141805966
11	37.21895683647733	21.389416333501902	21.418916125174533	19.972710704846236
12	24.25574649580642	23.381487171168764	28.576153990502817	23.786612342522
13	29.993893304580975	19.376910330175026	24.677251605392975	25.95194475985102
14	23.448279152314342	19.21334949063163	24.976702320862316	32.361669036191714
15	25.34254744616089	27.023638000184473	21.82030412615415	25.813510427500486
16	25.68469949874049	25.943996335971093	23.56690987506679	24.804394290221623
17	24.312758222569965	25.907575800947175	24.955869583885956	24.823796392596904
18	24.726013509484073	25.498495191371106	25.51169456956907	24.263796729575752
19	25.652255501591874	25.094224401973225	24.93702470349131	24.316495392943587
20	25.79132594804856	24.825863763441884	24.893848458536493	24.48896182997306
21	26.10948327614928	24.54782190381152	24.601414559868324	24.741280260170875
22	25.908291429316595	24.39600965621213	25.020037594343673	24.675661320127602
23	25.117144481506955	24.55879488086766	25.021886318367415	25.30217431925797
24	24.96358200410299	24.965728916524867	25.089534199519726	24.98115487985242
25	25.269195966394037	24.476596529673973	25.14165488528459	25.1125526186474
26	24.719234243403477	25.231625785603416	25.35399862598916	24.695141345003943
27	25.110366499473614	24.816877486824403	25.102176478259068	24.970579535442912
28	25.08019153730795	24.86804377177735	25.093470651567173	24.958294039347525
29	24.849439024196307	25.029539689733866	25.05649515358253	25.0645261324873
30	25.026240207631606	25.103847609596603	25.026717302315816	24.843194880455975
31	25.237033526607235	24.74547322419666	24.859894976893006	25.1575982723031
32	25.119888679057752	24.992982804890172	24.815584931915318	25.07154358413677
33	25.04355404160037	24.732095929416612	24.986860236650916	25.237489792332102
34	25.224788967131673	24.94362384941763	25.100427472758152	24.731159710692545
35	25.32119837727033	24.760939987309502	25.387354349516634	24.53050728590353
36	24.67985493383205	25.01636009869334	25.16998202178219	25.133802945692423
37	25.266298349570455	24.89058628575427	24.93113933391221	24.911976030763064
38	25.100347000244906	24.909512768400397	25.008428511906466	24.981711719448235
39	25.433952542329752	24.735016610661674	24.8400557876507	24.99097505935787
40	25.612459586811426	24.790121768336416	24.805229502383046	24.792189142469113
41	24.85573906841272	24.933743739151108	25.301264216371123	24.909252976065048
42	25.43109172264133	25.02276227910649	25.017832108610065	24.52831388964211
43	25.231079473526847	24.49020973682704	25.033556620037277	25.245154169608835
44	24.657557121523684	25.126154795472583	25.1137500377709	25.102538045232837
45	24.959148475313754	25.10951389269905	24.967020593939424	24.964317038047778
46	24.930661798149497	24.882395412228334	24.875477495300586	25.311465294321582
47	24.967895580125873	25.00423420522179	25.181394942013256	24.846475272639083
48	25.237900227821225	25.018030877131597	24.634352896271764	25.10971599877541
49	25.103074051897632	25.174400898225258	24.65467707704651	25.06784797283059
50	25.20562846292326	25.19155412497376	24.625718029783524	24.97709938231945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	9.5
2	11.0
3	55.5
4	100.0
5	115.5
6	131.0
7	111.0
8	91.0
9	114.0
10	137.0
11	252.0
12	367.0
13	711.0
14	1055.0
15	1431.5
16	1808.0
17	1827.0
18	1846.0
19	1954.5
20	2063.0
21	2458.0
22	2853.0
23	3587.0
24	4321.0
25	5763.5
26	7206.0
27	9283.0
28	11360.0
29	13495.0
30	15630.0
31	17769.0
32	19908.0
33	22982.0
34	26056.0
35	30633.0
36	35210.0
37	40325.0
38	45440.0
39	49649.0
40	53858.0
41	58011.5
42	62165.0
43	65465.0
44	68765.0
45	74300.5
46	79836.0
47	84437.5
48	89039.0
49	93141.0
50	97243.0
51	97340.5
52	97438.0
53	96960.0
54	96482.0
55	95184.5
56	93887.0
57	90558.0
58	87229.0
59	80486.5
60	73744.0
61	65258.5
62	56773.0
63	49478.5
64	42184.0
65	35126.5
66	28069.0
67	23840.0
68	19611.0
69	16532.5
70	13454.0
71	10577.5
72	7701.0
73	6700.5
74	5700.0
75	4527.0
76	3354.0
77	2794.0
78	2234.0
79	1848.5
80	1463.0
81	1177.0
82	891.0
83	689.5
84	488.0
85	367.0
86	246.0
87	169.0
88	92.0
89	56.5
90	21.0
91	17.5
92	14.0
93	16.5
94	19.0
95	18.0
96	17.0
97	15.5
98	14.0
99	9.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030374448568584234
2	0.004532313006307071
3	4.770855796112707E-4
4	0.0
5	0.0
6	1.590285265370902E-4
7	0.0
8	0.0
9	0.0
10	1.590285265370902E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.180570530741804E-4
17	0.0
18	7.95142632685451E-5
19	0.0
20	0.0
21	7.95142632685451E-5
22	0.0
23	7.95142632685451E-5
24	0.0012722282122967217
25	1.590285265370902E-4
26	3.180570530741804E-4
27	6.361141061483608E-4
28	0.0017493137919079925
29	4.770855796112707E-4
30	0.0019083423184450827
31	6.361141061483608E-4
32	8.746568959539962E-4
33	2.3854278980563534E-4
34	9.541711592225414E-4
35	1.590285265370902E-4
36	2.3854278980563534E-4
37	0.0019083423184450827
38	0.0
39	7.951426326854511E-4
40	1.590285265370902E-4
41	0.001510771002102357
42	0.005804541218603793
43	0.004770855796112707
44	0.004293770216501435
45	0.0026239706878619885
46	0.0025444564245934434
47	0.0016697995286394471
48	0.0056455126920667035
49	0.00357814184708453
50	0.0022263993715192633
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1257636.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.46959475410611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.37145663507206	37.092636737793484
2	16.366415799092824	17.502112407093858
3	6.235057594559319	10.001580085487953
4	2.9460032990532112	6.300864101785394
5	1.5648395131658797	4.183566731209614
6	0.9415597407392247	3.0206890664464527
7	0.5898452929169427	2.2077152145920156
8	0.40306924731452703	1.7241559449400263
9	0.27654446205155875	1.3308048285650207
>10	1.214880302723833	10.970812541446644
>50	0.05950649039536092	2.2478289381685865
>100	0.02947505331243049	2.984990214873247
>500	0.0013465696028258092	0.4322431875976661
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.590285265370902E-4
2	0.0	0.0	0.0	0.0	1.590285265370902E-4
3	0.0	0.0	0.0	0.0	1.590285265370902E-4
4	0.0	0.0	0.0	0.0	1.590285265370902E-4
5	0.0	0.0	0.0	0.0	1.590285265370902E-4
6	0.0	0.0	0.0	0.0	2.3854278980563534E-4
7	0.0	0.0	0.0	0.0	2.3854278980563534E-4
8	0.0	0.0	0.0	0.0	2.3854278980563534E-4
9	0.0	0.0	0.0	0.0	2.3854278980563534E-4
10	0.0	0.0	0.0	0.0	2.3854278980563534E-4
11	0.0	0.0	0.0	0.0	3.9757131634272555E-4
12	0.0	0.0	0.0	0.0	0.0011131996857596316
13	0.0	0.0	0.0	0.0	0.0013517424755652668
14	0.0	0.0	0.0	0.0	0.001431256738833812
15	0.0	0.0	0.0	1.590285265370902E-4	0.001510771002102357
16	0.0	0.0	0.0	1.590285265370902E-4	0.001510771002102357
17	0.0	0.0	0.0	3.180570530741804E-4	0.001510771002102357
18	0.0	0.0	0.0	3.9757131634272555E-4	0.0018288280551765376
19	0.0	0.0	0.0	6.361141061483608E-4	0.0018288280551765376
20	0.0	0.0	0.0	7.951426326854511E-4	0.0018288280551765376
21	0.0	0.0	0.0	9.541711592225414E-4	0.0018288280551765376
22	0.0	0.0	0.0	0.0012722282122967217	0.001987856581713628
23	0.0	0.0	0.0	0.0017493137919079925	0.002067370844982173
24	0.0	0.0	0.0	0.0026239706878619885	0.002067370844982173
25	0.0	0.0	0.0	0.00357814184708453	0.002146885108250718
26	0.0	0.0	0.0	0.004532313006307072	0.0026239706878619885
27	0.0	0.0	0.0	0.005088912849186887	0.0026239706878619885
28	0.0	0.0	0.0	0.006281626798215064	0.0026239706878619885
29	0.0	0.0	0.0	0.007712883537048876	0.0026239706878619885
30	0.0	0.0	0.0	0.01073442554125359	0.0026239706878619885
31	0.0	0.0	0.0	0.015982366916977568	0.0026239706878619885
32	0.0	0.0	0.0	0.020753222713090273	0.0026239706878619885
33	0.0	0.0	0.0	0.025921649825545706	0.0027034849511305336
34	0.0	0.0	0.0	0.03435016173201149	0.002862513477667624
35	0.0	0.0	0.0	0.041426931162912004	0.002862513477667624
36	0.0	0.0	0.0	0.05255892802050832	0.002862513477667624
37	0.0	0.0	0.0	0.06647392409250372	0.002862513477667624
38	0.0	0.0	0.0	0.08492123317080617	0.003021542004204714
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACG	30	0.0057456577	29.332752	4
GGTATCA	675	0.0	24.450762	1
ATCGATT	95	2.6861017E-8	23.157436	29
GTATCAA	1265	0.0	22.7885	1
CTATAGG	135	2.590241E-9	19.560612	1
GTTATAG	195	0.0	19.184444	1
TAGGACG	145	6.3082553E-9	18.206535	4
GTATAGA	170	1.2551027E-10	18.12233	1
TTGCGAG	75	0.0012910818	17.599651	11
ATTGCGA	75	0.0012910818	17.599651	10
GATATAC	340	0.0	17.475105	1
ACCGTCG	180	2.8558134E-10	17.110771	8
GCATATA	250	0.0	16.724321	1
CCTAATA	135	1.0853037E-6	16.296621	2
ACGGACC	360	0.0	15.888573	8
GACGGAC	305	0.0	15.868537	7
CCTACAC	250	0.0	15.840314	3
CGGATCG	140	1.5865808E-6	15.713972	26
TACCGTC	225	2.5465852E-11	15.644133	7
CGTCGTA	185	7.568815E-9	15.459151	10
>>END_MODULE