FastQCFastQC Report
Thu 2 Feb 2017
SRR4062109_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062109_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101218
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTACACCTACTACCCAACTATC1700.16795431642593214No Hit
GTATTGGAATTAGTGAAATTGGAGT1260.12448378746863208No Hit
GTGTAAATGTATGTGGTAAAAGGCC1180.11658005493094113No Hit
GTATCAACGCAGAGTACTTTTTTTT1080.10670038925882748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCT400.005234068714.254
ACATATA400.005234068714.252
GTACATG1350.005248257.0370371
Produced by FastQC (version 0.11.5)