##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062109_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101218
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25871880495564	32.0	32.0	32.0	32.0	32.0
2	30.910747100318126	32.0	32.0	32.0	32.0	32.0
3	30.92876761050406	32.0	32.0	32.0	32.0	32.0
4	30.925023217214328	32.0	32.0	32.0	32.0	32.0
5	30.904710624592465	32.0	32.0	32.0	32.0	32.0
6	34.50609575371969	36.0	36.0	36.0	32.0	36.0
7	34.42526032919046	36.0	36.0	36.0	32.0	36.0
8	34.3909877689739	36.0	36.0	36.0	32.0	36.0
9	34.469491592404516	36.0	36.0	36.0	32.0	36.0
10	34.290531328419846	36.0	36.0	36.0	32.0	36.0
11	34.48697860064416	36.0	36.0	36.0	32.0	36.0
12	34.35584579817819	36.0	36.0	36.0	32.0	36.0
13	34.41098421229425	36.0	36.0	36.0	32.0	36.0
14	34.35905668952163	36.0	36.0	36.0	32.0	36.0
15	34.28218301092691	36.0	36.0	36.0	32.0	36.0
16	34.317166907071865	36.0	36.0	36.0	32.0	36.0
17	34.285235827619594	36.0	36.0	36.0	32.0	36.0
18	34.266217471200775	36.0	36.0	36.0	32.0	36.0
19	34.28387243375684	36.0	36.0	36.0	32.0	36.0
20	34.22204548598076	36.0	36.0	36.0	32.0	36.0
21	34.244304372740025	36.0	36.0	36.0	32.0	36.0
22	34.19050959315537	36.0	36.0	36.0	32.0	36.0
23	34.17477128573969	36.0	36.0	36.0	32.0	36.0
24	34.14882728368472	36.0	36.0	36.0	32.0	36.0
25	33.80552866091011	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	8.0
6	27.0
7	2.0
8	24.0
9	15.0
10	6.0
11	1.0
12	6.0
13	7.0
14	11.0
15	17.0
16	34.0
17	46.0
18	72.0
19	76.0
20	106.0
21	150.0
22	253.0
23	358.0
24	522.0
25	789.0
26	1003.0
27	1338.0
28	1794.0
29	2447.0
30	3328.0
31	4429.0
32	6356.0
33	9219.0
34	21217.0
35	47556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26201132220872	22.03286009267218	12.745882608652698	24.959245976466406
2	15.00424825623901	22.234187595091782	39.480131992333376	23.28143215633583
3	17.290402892766107	27.518820763105378	29.542176292754252	25.64860005137426
4	11.797369799128536	18.076455651177266	40.30471598375638	29.821458565937814
5	13.082366428811449	37.63811745172066	36.180348283291494	13.099167836176395
6	28.826210319225716	39.901731075323035	18.449644590760347	12.822414014690908
7	25.80715359658147	31.824206694626888	24.93966131202026	17.428978396771384
8	23.931277323890722	36.13281636367233	22.219145021859113	17.716761290577832
9	24.26322922746654	16.876230424502637	22.667510857414204	36.193029490616624
10	14.81148035655985	28.07166813419472	34.91793385240955	22.198917656835878
11	31.506456241033	25.59738781971998	24.91070103398803	17.98545490525899
12	20.074603237424306	27.499307397000038	31.432303003918154	20.9937863616575
13	26.2132080138511	23.017561216918132	27.454860252287904	23.314370516942866
14	20.552159376421944	21.788632361960154	28.712881080974146	28.946327180643756
15	22.941444204777074	30.590900995389152	23.33227791740051	23.135376882433263
16	22.04987684119934	29.479963200743896	26.204631562286696	22.265528395770065
17	19.544914918876138	29.952512861100118	28.25583696082311	22.246735259200634
18	19.935309653105435	30.090902796324322	28.729833724047204	21.243953826523043
19	21.46219883688729	28.324168216164892	28.8394587965344	21.37417415041342
20	21.977826349259725	28.79113053970389	28.45387742184332	20.777165689193065
21	22.282769942836797	28.68247720394802	27.08329212571948	21.951460727495697
22	21.458951533135508	27.938674579624134	28.79327398615232	21.80909990108803
23	21.8847028577079	28.934045288242853	27.702956590527045	21.4782952635222
24	21.142263759086187	28.337041981901795	28.340008900756565	22.180685358255452
25	21.272387432380313	28.351315802486226	28.57284136198661	21.80345540314685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	14.0
2	29.5
3	45.0
4	45.0
5	45.0
6	77.0
7	109.0
8	109.0
9	109.0
10	176.5
11	244.0
12	244.0
13	244.0
14	452.5
15	661.0
16	661.0
17	661.0
18	1322.0
19	1983.0
20	1983.0
21	1983.0
22	2637.0
23	3291.0
24	3291.0
25	3291.0
26	4631.5
27	5972.0
28	5972.0
29	5972.0
30	7335.0
31	8698.0
32	8698.0
33	8698.0
34	9937.0
35	11176.0
36	11176.0
37	11176.0
38	11981.0
39	12786.0
40	12786.0
41	12786.0
42	12540.5
43	12295.0
44	12295.0
45	12295.0
46	12294.5
47	12294.0
48	12294.0
49	12294.0
50	11191.5
51	10089.0
52	10089.0
53	10089.0
54	9158.5
55	8228.0
56	8228.0
57	8228.0
58	7023.0
59	5818.0
60	5818.0
61	5818.0
62	4812.0
63	3806.0
64	3806.0
65	3806.0
66	2915.0
67	2024.0
68	2024.0
69	2024.0
70	1507.5
71	991.0
72	991.0
73	991.0
74	693.5
75	396.0
76	396.0
77	396.0
78	293.0
79	190.0
80	190.0
81	190.0
82	121.0
83	52.0
84	52.0
85	52.0
86	34.0
87	16.0
88	16.0
89	16.0
90	15.5
91	15.0
92	15.0
93	15.0
94	9.0
95	3.0
96	3.0
97	3.0
98	12.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.879665672113656E-4
2	0.0
3	0.0
4	0.008891699104902291
5	0.035566796419609165
6	0.06619376000316149
7	0.12053192119978659
8	0.11460412179651841
9	0.13337548657353435
10	0.13930328597680255
11	0.15115888478333894
12	0.14819498508170484
13	0.14127921911122526
14	0.12250785433420933
15	0.15017091821612755
16	0.12744768717026617
17	0.13633938627516845
18	0.11954395463257524
19	0.1086763223932502
20	0.10571242269161613
21	0.10274852298998202
22	0.11658005493094113
23	0.08694105791460018
24	0.10176055642277067
25	0.09978462328834792
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	101218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.96571756011777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.40470216098667	54.83412041336522
2	8.426479310187508	10.948645497836353
3	2.221816688717551	4.330257464087415
4	1.1709779947382026	3.042937027011006
5	0.8288091001718447	2.692208895650971
6	0.6174247608619615	2.4066865577268866
7	0.41668567604969814	1.894919875911399
8	0.2980671259333607	1.5491315773874212
9	0.2661313624405006	1.5560473433579007
>10	1.3002417993521602	14.298840127250093
>50	0.0425810179904801	1.9304866723310083
>100	0.006083002570068586	0.5157185480843328
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATTTACACCTACTACCCAACTATC	170	0.16795431642593214	No Hit
GTATTGGAATTAGTGAAATTGGAGT	126	0.12448378746863208	No Hit
GTGTAAATGTATGTGGTAAAAGGCC	118	0.11658005493094113	No Hit
GTATCAACGCAGAGTACTTTTTTTT	108	0.10670038925882748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.879665672113656E-4	0.0
11	0.0	0.0	0.0	9.879665672113656E-4	0.0
12	0.0	0.0	0.0	9.879665672113656E-4	0.0
13	0.0	0.0	0.0	9.879665672113656E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCT	40	0.0052340687	14.25	4
ACATATA	40	0.0052340687	14.25	2
GTACATG	135	0.00524825	7.037037	1
>>END_MODULE