FastQCFastQC Report
Thu 2 Feb 2017
SRR4062109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101218
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA1680.1659783832915094No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT1210.11954395463257524No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT1130.1116402220948843No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT1030.10176055642277067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAT207.8259176E-443.99604812
GGTGCTA301.2896498E-436.68149644
CTACATC250.002343685835.196843
TTCGGCA250.002343685835.1968435
TGCCCGG401.8048371E-532.99703612
TTAGGCA300.005723004729.3306984
TACCATG300.005723004729.3306987
GATCGGA300.005723004729.33069824
GGATCGG300.005723004729.33069823
CTAGTAC300.005723004729.3306983
ACATCTA300.005723004729.3306985
ATCGGAT300.005723004729.33069825
TAGTACC454.0357176E-529.3306984
CTATAGA406.9306494E-427.5383471
CTTCGGC406.9910736E-427.4975334
TACATCT406.9910736E-427.497534
CGCTTCG508.272584E-526.39762932
GGTATCA450.001378142924.478531
GTGTAAA450.001378142924.478531
GAATGAG450.001390118224.4422520
Produced by FastQC (version 0.11.5)